Characterization of a carbofuran-degrading bacterium and investigation of the role of plasmids in catabolism of the insecticide carbofuran

Head, Ian M.; Cain, Ronald B.; Suett, D. L.

doi:10.1007/bf00245249

Cited by 22 publications

(13 citation statements)

References 34 publications

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“…Several bacteria competent of degrading either carbofuran, carbarly, baygon or aldicarb have been isolated and characterized [9,10,11,12,13,14,15,16,17]. In another example i.e.…”

Section: Recent Developments In Pesticide Biodegradationmentioning

confidence: 99%

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Singh

2008

Indian J Microbiol

165

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Biodegradation is a natural process, where the degradation of a xenobiotic chemical or pesticide by an organism is primarily a strategy for their own survival. Most of these microbes work in natural environment but some modifi cations can be brought about to encourage the organisms to degrade the pesticide at a faster rate in a limited time frame. This capability of microbe is some times utilized as technology for removal of contaminant from actual site. Knowledge of physiology, biochemistry and genetics of the desired microbe may further enhance the microbial process to achieve bioremediation with precision and with limited or no scope for uncertainty and variability in microbe functioning. Gene encoding for enzyme has been identifi ed for several pesticides, which will provide a new inputs in understanding the microbial capability to degrade a pesticide and develop a super strain to achieve the desired result of bioremediation in a short time.

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“…Several bacteria competent of degrading either carbofuran, carbarly, baygon or aldicarb have been isolated and characterized [9,10,11,12,13,14,15,16,17]. In another example i.e.…”

Section: Recent Developments In Pesticide Biodegradationmentioning

confidence: 99%

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Singh

2008

Indian J Microbiol

165

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“…It is now generally accepted that adapted soil microorganisms are responsible for the rapid degradation of soilapplied pesticides following repeated treatments with the same or similar compounds (Racke and Coats 1990). Several authors have reported the isolation and characterization of carbofuran-degrading fungi (Williams et al 1976), actinomycetes (Williams et al 1976 ;Venkateswarlu andSethunathan 1984, 1985) and bacteria belonging to several different genera (Felsot et al 1981;Rajagopal et al 1984;Karns et al 1986;Chaudry and Ali 1988;Ramanand et al 1988;Head et al 1992;Parekh et al 1992). Other workers have used several different enrichment techniques and have usually succeeded in isolating only one or two types of carbofuran-degrading bacteria from any one soil.…”

Section: Carbofuranmentioning

confidence: 99%

Carbofuran‐degrading bacteria from previously treated field soils

Parekh¹,

Suett²,

Roberts³

et al. 1994

Journal of Applied Bacteriology

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Laboratory incubation studies were made on soils collected from five field sites with different histories of treatment with carbofuran. All soils treated earlier with carbofuran degraded the compound more rapidly than untreated samples of the same soils. Reduced rates of degradation in the presence of chloramphenicol imply that soil bacteria are primarily responsible for the breakdown of carbofuran in these soils. Sixty-eight bacteria, capable of degrading carbofuran as the sole source of carbon and nitrogen, were isolated from liquid cultures of treated soils. The concentration of carbofuran in the liquid medium used for isolation and subsequent culture of carbofuran-degrading isolates appeared to affect the stability of their ability to degrade. Similar types of carbofuran-degrading bacteria were isolated from different soils and several different types were isolated from one soil. All carbofuran-degrading isolates were Gram-negative, aerobic rods which hydrolysed the insecticide to carbofuran phenol. They were separated into four groups on the basis of a limited number of phenotypic characters. There was a good correlation between the phenotype of carbofuran-degrading isolates and the stability of their ability to degrade. Fourteen isolates were placed in phenotypic group I and 13 of these did not degrade carbofuran after one subculture in liquid medium. Phenotypic groups II, III and IV consisted of 54 isolates in total (3, 46 and 5 isolates respectively) and 52 of these retained their ability to degrade carbofuran when subcultured.

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“…Some microorganisms such as Pseudomonas, Flavobacterium, Achromobacterium, and Burkholderia are known to rapidly degrade carbofuran in the environment [2,3,5,10,14,24,26]. The growth of the degrading microbial populations was roughly stimulated in proportion to increasing concentrations of the pesticide in soils.…”

mentioning

confidence: 99%

“…Therefore, the detoxification of carbofuran is needed for a better agricultural environment and very important for nontarget organisms in terrestrial and aquatic environments exposed to the pesticide. Bacterial populations are reported to perform a decisive role in the detoxification of carbofuran in soil [3,8,10,12,13,25,30] and a carbofuran hydrolase gene (mcd) was cloned from plasmid DNA of Achromobacter sp. WM111 [29].…”

mentioning

confidence: 99%

Genetic and Phenotypic Diversity of Carbofuran-Degrading Bacteria Isolated from Agricultural Soils

Shin¹

2012

J. Microbiol. Biotechnol.

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Thirty-seven carbofuran-degrading bacteria were isolated from agricultural soils, and their genetic and phenotypic characteristics were investigated. The isolates were able to utilize carbofuran as a sole source of carbon and energy. Analysis of the 16S rRNA gene sequence indicated that the isolates were related to members of the genera Rhodococcus, Sphingomonas, and Sphingobium, including new types of carbofuran-degrading bacteria, Bosea and Microbacterium. Among the 37 isolates, 15 different chromosomal DNA patterns were obtained by polymerase chain reaction (PCR) amplification of repetitive extragenic palindromic (REP) sequences. Five of the 15 representative isolates were able to degrade carbofuran phenol, fenoxycarb, and carbaryl, in addition to carbofuran. Ten of the 15 representative isolates had 1 to 8 plasmids. Among the 10 plasmid-containing isolates, plasmid-cured strains were obtained from 5 strains. The cured strains could not degrade carbofuran and other pesticides anymore, suggesting that the carbofuran degradative genes were on the plasmid DNAs in these strains. When analyzed with PCR amplification and dot-blot hybridization using the primers targeting for the previously reported carbofuran hydrolase gene (mcd), all of the isolates did not show any positive signals, suggesting that their carbofuran hydrolase genes had no significant sequence homology with the mcd gene.

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Characterization of a carbofuran-degrading bacterium and investigation of the role of plasmids in catabolism of the insecticide carbofuran

Cited by 22 publications

References 34 publications

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Carbofuran‐degrading bacteria from previously treated field soils

Genetic and Phenotypic Diversity of Carbofuran-Degrading Bacteria Isolated from Agricultural Soils

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