Characterization of a cell death‐inducing endonuclease‐like venom protein from the parasitoid wasp <i>Pteromalus puparum</i> (<scp>Hymenoptera: Pteromalidae</scp>)

Wang, Jiale; Yan, Zhichao; Xiao, Shan; Wang, Beibei; Fang, Qi; Schlenke, Todd A.; Yè, Gōngyín

doi:10.1002/ps.6011

Cited by 4 publications

(6 citation statements)

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“…Melectin manifests antitumour activity, penetrates membranes and binds DNA [ 38 ]. The venom of a parasitoid wasp, Pteromalus puparum, contains a peptide with endonuclease activity (PpENVP), which inhibits gene expression in transfected cells relying on two activation sites [ 71 ].…”

Section: Results Of Literature Searchmentioning

confidence: 99%

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Sjakste

Gajski

2023

Toxins

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Envenomation by animal venoms remains a serious medical and social problem, especially in tropical countries. On the other hand, animal venoms are widely used as a source of biologically active compounds for the development of novel drugs. Numerous derivatives of animal venoms are already used in clinical practice. When analysing the mechanisms of action of animal venoms, attention is usually focused on the main target of the venom’s enzymes and peptides such as neurotoxic, cytotoxic or haemorrhagic effects. In the present review, we would like to draw attention to the “hidden” effects of animal venoms and their derivatives in regard to DNA damage and/or protection against DNA damage. Alkaloids and terpenoids isolated from sponges such as avarol, ingenamine G or variolin B manifest the capability to bind DNA in vitro and produce DNA breaks. Trabectidin, isolated from a sea squirt, also binds and damages DNA. A similar action is possible for peptides isolated from bee and wasp venoms such as mastoparan, melectin and melittin. However, DNA lesions produced by the crude venoms of jellyfish, scorpions, spiders and snakes arise as a consequence of cell membrane damage and the subsequent oxidative stress, whereas certain animal venoms or their components produce a genoprotective effect. Current research data point to the possibility of using animal venoms and their components in the development of various potential therapeutic agents; however, before their possible clinical use the route of injection, molecular target, mechanism of action, exact dosage, possible side effects and other fundamental parameters should be further investigated.

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Section: Results Of Literature Searchmentioning

confidence: 99%

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Sjakste

Gajski

2023

Toxins

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“…The virulence factors of a natural enemy help overcome host immunity, thus establishing successful parasitism and host killing. 9,10,14,[16][17][18] For instance, braconid and ichneumonid parasitoids release proteins, venom, and polydnaviruses into the host hemolymph to suppress host immunity. 14,[16][17][18] Several serine proteinases were found in the host hemolymph, which help entomopathogenic nematodes overcome host immunity and enable host killing.…”

Section: Discussionmentioning

confidence: 99%

“…9,10,14,[16][17][18] For instance, braconid and ichneumonid parasitoids release proteins, venom, and polydnaviruses into the host hemolymph to suppress host immunity. 14,[16][17][18] Several serine proteinases were found in the host hemolymph, which help entomopathogenic nematodes overcome host immunity and enable host killing. [9][10][11][12] Our observations on the host hemolymph revealed that a mixture of proteins is involved in O. sinensis parasitism, similar to that of S. carpocapsae.…”

Section: Discussionmentioning

confidence: 99%

“…[8][9][10][11][12][13] Interestingly, parasitoid wasps use virulent proteins, venom, and polydnaviruses to overcome host immunity and kill the host. [14][15][16][17][18] 18 Therefore, parasites passively escape or actively control host immunity using virulence factors. 1,3 Mermithid nematodes are entomopathogens that infect a broad range of insect pests, including lepidopteran pests and mosquitoes, thus providing biological control of insect pests.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, entomopathogenic nematodes inhibit host immune responses and kill the host by secreting toxin proteases or by releasing symbiotic pathogenic bacteria 8–13 . Interestingly, parasitoid wasps use virulent proteins, venom, and polydnaviruses to overcome host immunity and kill the host 14–18 . Therefore, parasites passively escape or actively control host immunity using virulence factors 1,3 …”

Section: Introductionmentioning

confidence: 99%

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Serine protease mediates Ovomermis sinensis‐inhibited host immune responses by inducing apoptosis: implications for successful parasitism and host mortality

Jiao,

Chen,

Zhao

et al. 2023

Pest Management Science

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BACKGROUNDMermithid nematodes are entomopathogens that parasitize and kill insect hosts and are used for biological control. It is widely believed that mermithid nematodes kill their host upon nematode emergence, unlike other parasites that depend on virulence factors. In this study, we disproved this theory by demonstrating that the mermithid nematode Ovomermis sinensis mediates host mortality by serine protease‐induced apoptosis.RESULTSSuccessful parasitism of O. sinensis increased with the infection rate, and the inhibition of host immunity by O. sinensis increased with the parasitic load. A serine protease was identified from the host hemolymph. This protease belongs to the trypsin‐like serine protease family, which is an apoptosis‐inducing serine protease. Specifically, Os‐sp was highly expressed only during the parasitic stage and could be induced by host hemocytes and the fat body. Importantly, host immune effectors (melanization, phenoloxidase activity, and encapsulation) were suppressed by the recombinant protein rOs‐sp that induced apoptosis of hemocytes and fat body in a dose‐dependent manner, which contributes to host death.CONCLUSIONSerine protease mediates O. sinensis‐inhibited host immune responses by inducing apoptosis that is lethal to the insect host. Our findings have broader implications for understanding the mechanism of successful parasitism and killing of host by nematodes. © 2023 Society of Chemical Industry.

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Unraveling the venom constituents of the endoparasitoid Aphidius gifuensis with an emphasis on the discovery of a novel insecticidal peptide

Wang,

Wu,

et al. 2024

Pest Management Science

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BACKGROUNDVenom serves as a pivotal parasitic factor employed by parasitoid wasps to manipulate their hosts, creating a favorable environment for the successful growth of their progeny, and ultimately kill the host. The bioactive molecules within parasitoid venoms exhibit insecticidal activities with promising prospects for agricultural applications. However, knowledge regarding the venom components of parasitoids and the discovery of functional biomolecules from them remains limited.RESULTSIn this study, 30 venom proteins were identified from the endoparasitoid Aphidius gifuensis through the application of a transcriptomic approach. These proteins were categorized into five groups: hydrolase, molecular chaperone, transferase, other functional protein, and hypothetical protein with unknown function. Particularly noteworthy is the abundant expression of the peptide Vn1 in the venom apparatus of A. gifuensis, indicating its pivotal role in venom activity. Consequently, Vn1 was chosen for further functional analysis, exhibiting insecticidal activity against Tenebrio molitor pupae. Further assessment for revealing its mode of action disclosed that Vn1 impacts genes related to immune response, environmental information processing, metabolism, and response to external stimuli in T. molitor, suggesting its involvement in the intricate parasitoid wasp–host interaction.CONCLUSIONThe findings of this study significantly contribute to our knowledge of the composition and functionality of A. gifuensis venom, establishing a foundation for further investigation into the biological roles of the identified venom constituents. The insecticidal Vn1 isolated from the venom of this parasitoid represents a valuable resource for the development of innovative biocontrol agents with potential applications in agriculture. © 2024 Society of Chemical Industry.

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Characterization of a cell death‐inducing endonuclease‐like venom protein from the parasitoid wasp Pteromalus puparum (Hymenoptera: Pteromalidae)

Cited by 4 publications

References 50 publications

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

A Review on Genotoxic and Genoprotective Effects of Biologically Active Compounds of Animal Origin

Serine protease mediates Ovomermis sinensis‐inhibited host immune responses by inducing apoptosis: implications for successful parasitism and host mortality

Unraveling the venom constituents of the endoparasitoid Aphidius gifuensis with an emphasis on the discovery of a novel insecticidal peptide

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