Characterization of a compact and highly sensitive fluorescence-based detection system for point-of-care applications

Kullman, Dixie E.; Smith, John E.; Katchman, Benjamin A.; Arafa, Hany; Anderson, Karen S.; Christen, Jennifer Blain

doi:10.1109/hic.2016.7797711

Cited by 8 publications

(3 citation statements)

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“…At the center of the system, providing sequencing and control is the microcontroller module (M4) implemented with an Arduino Uno, that monitors, acquires, and digitizes the amplifier output voltage, recording time taken for the output voltage to rise to the voltage rail of the amplifier (ramp-time, RT). The RT is inversely correlated with the sample concentration as a higher fluorophore concentration implies more light, a steeper slope, and thus, a smaller RT (Obahiagbon et al, 2016). We define detection time (DT) as the ramp time different between a negative control or reference sample and a sample under test.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we reported the performance of a fluorescence detection system that uses relatively expensive 25 mm fi in a single site configuration (Katchman et al, 2016; Obahiagbon et al, 2016; Smith et al, 2016). Here, we discuss the use of 3×3 mm filter The 3×3 mm fi were mounted directly on the LEDs and photodiodes using optically clear UV curing adhesive, (NOA63, Norland Prodcuts Inc., NJ, USA) as shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we have reported the use of organic light emitting diodes (OLEDs), adapted from flat panel display technology, as the excitation source in a single-plex detection system (Katchman et al, 2016; Obahiagbon et al, 2016; Smith et al, 2016). We demonstrated the detection of antibodies to human papillomavirus (HPV16) E7 protein using expensive interference filters Compared to single-plex systems, low-cost, multiplexed diagnostic platforms are useful for disease diagnosis, making it possible for simultaneous detection of multiple analytes from a single patient sample volume.…”

Section: Introductionmentioning

confidence: 99%

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A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications

Smith

Zhu

Katchman

et al. 2018

Biosensors and Bioelectronics

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An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3×3 mm interference filter with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of ∼1×104 photons/mm2sec and has the potential for 2 to 3 orders of magnitude improvement in sensitivity over standard colorimetric detection that uses colored latex microspheres. We also detail the design, development, and characterization of our low-cost fluorescence detection platform and demonstrate 100% and 97.96% reduction in crosstalk probability and filter cost, respectively. This is achieved by reducing fi r dimensions and ensuring appropriate channel isolation in a 2×2 array configuration. Practical considerations with low-cost interference fi system design, analysis, and system performance are also discussed. The performance of our platform is compared to that of a standard laboratory array scanner. We also demonstrate the detection of antibodies to human papillomavirus (HPV16) E7 protein, as a potential biomarker for early cervical cancer detection in human plasma.

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Section: Methodsmentioning

confidence: 99%