Characterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress

Li, Guilin; Zhao, Hang; Wang, Hongfang; Guo, Xing; Guo, Xingqi; Sun, Qinghua; Xu, Baohua

doi:10.1371/journal.pone.0149117

Cited by 7 publications

(7 citation statements)

References 74 publications

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“…The total protein was extracted from the honeybee samples using a Tissue Protein Extraction Kit (CWbiotech, Beijing, China). Western blotting was performed according to the procedure described by Li et al [30] with some modifications. The protein samples were separated by 12% SDS-PAGE and electrotransferred onto a polyvinylidene difluoride (PVDF) membrane.…”

Section: Methodsmentioning

confidence: 99%

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Wang

Cui

et al. 2018

Bioscience Reports

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Mitogen-activated protein kinase kinases (MKKs) are important components of the MAPK signaling pathways, which play a key role in responding to stress and inflammatory stimuli. Here, a new MKK gene, AccMKK6, was identified and functionally analyzed in Apis cerana cerana. Real-time quantitative PCR (qPCR) and Western blot analysis demonstrated that the AccMKK6 expression level was up-regulated by several environmental stresses. Moreover, the knockdown of AccMKK6 by RNA interference technology altered the expression levels of some antioxidant genes. In addition, the knockdown of AccMKK6 resulted in increased malonyldialdehyde (MDA) concentration and decreased antioxidant-related enzymes activity in honeybees. To explore the MAPK signaling pathways involved in AccMKK6, we identified the transcription factor kayak in A. cerana cerana. We analyzed the interactions of AccMKK6, Accp38b, and Acckayak using the yeast two-hybrid system. AccMKK6 and Acckayak showed similar expression profiles after several stress treatments. In addition, the expression level of Acckayak was significantly increased when AccMKK6 was silenced. Therefore, we speculate that AccMKK6 may be involved in the MAPK cascades, which play a crucial role in counteracting oxidative stress caused by external stimuli.

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Section: Methodsmentioning

confidence: 99%

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Wang

Cui

et al. 2018

Bioscience Reports

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“…After RNAi of AccDnaJA1 , AccDnaJB12 , and AccDnaJC8 , qRT-PCR was used to detect the mRNA levels of AccANT , AccCDK5r , AccCPR24 , AccERR , AccTP1 , AccCDK5 , AccCYP336A1 , AccSp10 , AccRBM11 , AccAK , AccMTNR1A , and AccAIF3 . These genes were proved to be involved in the stress response in our previous studies ( Chu et al, 2013 ; Liu X. et al, 2014 ; Wang et al, 2014 ; Chen et al, 2015 ; Li et al, 2016b , 2018a ; Zhang et al, 2016 ; Zhou et al, 2016 ; Zhu et al, 2016 ; Gao et al, 2017 ; Zhao et al, 2018 ).…”

Section: Methodsmentioning

confidence: 79%

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Zhao

Zhang

et al. 2018

Front. Genet.

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DnaJ, also known as Hsp40, plays important roles in maintaining the normal physiological state of an organism under stress conditions by mediating essential processes, such as protein synthesis, degradation, folding and metabolism. However, the exact functions of most DnaJ members are not fully understood in insects. Here, we identified three genes, AccDnaJA1, AccDnaJB12, and AccDnaJC8, in Apis cerana cerana and explored their connection with the environmental stress response. Quantitative real-time PCR results showed that the mRNA levels of AccDnaJA1, AccDnaJB12, and AccDnaJC8 were all induced under cold, UV, H2O2 and different pesticides treatment. The expression patterns of AccDnaJB12 and AccDnaJC8 were upregulated by CdCl2 and HgCl2 stress, while the transcriptional levels of AccDnaJA1 were downregulated by CdCl2 and HgCl2 stress. Western blot findings further indicated that AccDnaJB12 protein levels were increased by some stress conditions. Knockdown of each of these three genes downregulated the transcriptional patterns of several stress response-related genes at different levels. Functional analysis further demonstrated that the resistance of A. cerana cerana to lambda-cyhalothrin stress was reduced with knockdown of AccDnaJA1, AccDnaJB12, or AccDnaJC8, indicating that these three genes may be involved in the tolerance to this pesticide. Taken together, these findings indicate that AccDnaJA1, AccDnaJB12, and AccDnaJC8 may play pivotal roles in the stress response by facilitating honeybee survival under some adverse circumstances. To our knowledge, this is the first report that reveals the roles of DnaJ family proteins under different adverse circumstances in A. cerana cerana.

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“…Therefore, we collected 15‐day‐old worker bees and divided them into six groups of 40 individuals each. The collected bees were transferred from their beehives to an incubator with a constant temperature of 34°C, 60% humidity, and 24 h of darkness and were provided with water, pollen, and 30% sucrose solutions (Li et al, 2016). The six groups of bees were then subjected to different stress environments that are strongly associated with oxidative stress.…”

Section: Methodsmentioning

confidence: 99%

Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response

Guo

Wang

et al. 2021

Arch Insect Biochem Physiol

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Zinc finger proteins (ZFPs) are a class of transcription factors that contain zinc finger domains and play important roles in growth, aging, and responses to abiotic and biotic stresses. These proteins activate or inhibit gene transcription by binding to single‐stranded DNA or RNA and through RNA/DNA bidirectional binding and protein–protein interactions. However, few studies have focused on the oxidation resistance functions of ZFPs in insects, particularly Apis cerana. In the current study, we identified a ZFP41 gene from A. cerana, AcZFP41, and verified its function in oxidative stress responses. Real‐time quantitative polymerase chain reaction showed that the transcription level of AcZFP41 was upregulated to different degrees during exposure to oxidative stress, including that induced by extreme temperature, UV radiation, or pesticides. In addition, the silencing of AcZFP41 led to changes in the expression patterns of some known antioxidant genes. Moreover, the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and glutathione S‐transferase (GST) in AcZFP41‐silenced honeybees were higher than those in a control group. In summary, the data indicate that AcZFP41 is involved in the oxidative stress response. The results provide a theoretical basis for further studies of zinc finger proteins and improve our understanding of the antioxidant mechanisms of honeybees.

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Characterization of a Decapentapletic Gene (AccDpp) from Apis cerana cerana and Its Possible Involvement in Development and Response to Oxidative Stress

Cited by 7 publications

References 74 publications

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Molecular mechanism by which Apis cerana cerana MKK6 (AccMKK6)-mediated MAPK cascades regulate the oxidative stress response

Environmental Stress Responses of DnaJA1, DnaJB12 and DnaJC8 in Apis cerana cerana

Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response

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