Characterization of a double conjugate in uremic body fluids

Self Cite

In a retrospective study of 58 nondialyzed uremic patients, artifacts originating from furosemide, methyldopa, hydralazine, allopurinol and β-blocking agents on determination of plasma middle molecules could not be substantiated. Furosemide administration did not have any influence on middle molecule determination in hemodialysis patients. The salicylic acid administration in normal subjects resulted in increased urinary excretion of fraction 7c; the role of salicylic acid needs to be further investigated.

Section: Discussionmentioning

confidence: 99%

On Drug Artifacts in Middle Molecule Analysis

Asaba

Zimmerman

Bergström

1985

Self Cite

“…Not surprisingly, a whole new series of substances are being reported, some having acidic natures and molecular weights of less than 500 daltons. A substance conjugated with glucuronic acid, benzoic acid, and glycine may have potential biological activity [23], and this will need evaluation. So far, investigators studying platelet function and bleeding defects in uremia have looked at whole serum [16] or confined themselves to selected fractions [17,18].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Platelet Function by Uremic Middle Molecules

Bazilinski¹,

Shaykh²,

Dunea³

et al. 1985

To further define the platelet abnormality responsible for uremic bleeding, we studied platelet aggregation with adenosine diphosphate, ristocetin, and collagen in serum fractions obtained by Sephadex G-15 chromatography. We found that uremic patients had considerable inhibition in several peaks of middle molecular range, but the findings were inconsistent and not clearly related to the degree of uremia.

“…This substance, which inhibits erythropoiesis in vitro, has not yet been identified but does not appear to be urea, creatinine, or guanidinosuccinic acid [29], This factor [60] in the serum of uremic patients remains to be determined. In all the other studies the separation pro cedures only permit a crude separation of M M which con tains a complex mixture of substances.…”

Section: Anemiamentioning

confidence: 99%

Are ‘Middle Molecules’ Responsible for Toxic Phenomena in Chronic Renal Failure?

Navarro¹,

Contreras²,

Touraine³

et al. 1982

Since ‘middle molecule’ (MM) hypothesis was formulated and despite the extensive studies in this field, results are still confusing concerning the role of so-called MM in uremic toxicity. In this review we summarize the multiple works that tend to correlate the major uremic symptoms with some substances known to accumulate in uremia, and more particularly the symptoms in which MM have been postulated to play a part.