Characterization of a G14 equine rotavirus (strain CH3) isolated in Japan

Abstract

Antigenic and genomic properties of equine rotavirus strain CH3 isolated in Japan were studied by cross-neutralization tests and nucleotide sequence determination of the VP4 and VP7 genes. It was shown that the strain CH3 belongs to G14 and shares VP4 genotype with strain H2.

Field Study of Inactivated Equine Rotavirus Vaccine

“…The serotypes and genotypes of rotaviruses are classified by differences in the antigenicity of two kinds of viral outer capsid protein (VP4 and VP7) or the VP4 coding gene. It has been confirmed that both the G3P [12] and G14P [12] rotaviruses spread in the Hidaka region of Hokkaido in 1996 and 1997 [14], although the G3P [12] rotavirus emerged between 1981 and 1991 [7,11,13]. It has been suggested that the G3P [12] rotavirus was predominant in surveys conducted on sera collected from yearlings in the same region in 1999, but the G14P [ 1 2 ] r o t a v i r u s h a s a l s o s p r e a d [ 8 ] .…”

Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan

“…Hyperimmune antisera were prepared in guinea pigs against equine GAR 3 reference strains (HO5, L338 and FI23) and 2 isolates (designated the JE29 and JE77) that had been propagated in MA104 cells and semipurified on sucrose gradients. Hyperimmune antisera to H2 and FI14 strains were prepared in rabbits as described previously [40]. These antisera were used for VN tests.…”

“…Serotype G3 equine GAR have been found to be predominant worldwide [1,2,19,25,27], with the predominant subtype being G3A [1,2]. Serotype G14 equine GAR have recently been reported [6,7,40], and the prevalence of this serotype in the UK and Australia was lower than that of G3 [1,27]. The other VP7 serotypes [G5, G8, G10, and G13] in equine GAR might be uncommon because at most very few strains of these serotypes have been detected [1,25,27].…”

Sero-Epidemiological Survey of Equine Rotavirus Infections in 1-year-old Thoroughbred Horses in the Hidaka Region of Hokkaido.

“…Diarrhea in horses caused by rotavirus occurs repeatedly from May through July each year in the Hidaka region of Hokkaido, where more than 90% of Japanese thoroughbred horses are reared [14]. Epidemiological surveys based on viruses isolated from foal diarrheal feces have shown that G3 and G14 equine rotaviruses have spread across the Hidaka region and the adjacent Iburi region [15,19,22,23]. The purpose of this study was to investigate the activity of G3, G14, and G13 equine rotaviruses among young horses in the Hidaka region by serological survey using the serum neutralization (SN) test.…”