Characterization of a glucansucrase from<i>Lactobacillus reuteri</i>E81 and production of malto-oligosaccharides

İspirli, Hümeyra; Yüzer, Mustafa Onur; Skory, Christopher D.; Colquhoun, Ian J.; Sağdıç, Osman; Dertli, Enes

doi:10.1080/10242422.2019.1593969

Cited by 25 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 ). The main characteristic peaks showed a broad peak between 3000 and 3600 cm −1 , demonstrating a hydroxyl group 45 , 46 —OH stretching vibration and appearing at 3308 cm −1 . A distinct characteristic peak appeared at 2928 cm −1 , which was related to the C-H stretch vibration in the sugar ring 45 , 47 .…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the C–O stretching vibration was illustrated at 1643 cm −1 48 . A peak emerged at 1346 cm −1 that could be assigned to the symmetric CH 3 bending 46 . Strong characteristic peaks emerged at 1006 cm −1 and 918 cm −1 in the region 950–1100 cm −1 , which was attributed to the C–O–C and C–O groups of polysaccharides 46 .…”

Section: Resultsmentioning

confidence: 99%

“…A peak emerged at 1346 cm −1 that could be assigned to the symmetric CH 3 bending 46 . Strong characteristic peaks emerged at 1006 cm −1 and 918 cm −1 in the region 950–1100 cm −1 , which was attributed to the C–O–C and C–O groups of polysaccharides 46 . The peak in the region 950–1100 cm −1 was due to the presence of the (1 → 6)- and (1 → 3)-linked α-D-glucose units, respectively 49 , 50 .…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Beneficial effects of a polysaccharide-based grinding aid on magnetite flotation: a green approach

Chipakwe

Karlkvist

Rosenkranz

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Grinding is the most energy-intensive step in mineral beneficiation processes. The use of grinding aids (GAs) could be an innovative solution to reduce the high energy consumption associated with size reduction. Surprisingly, little is known about the effects of GAs on downstream mineral beneficiation processes, such as flotation separation. The use of ecofriendly GAs such as polysaccharide-based materials would help multiply the reduction of environmental issues in mineral processing plants. As a practical approach, this work explored the effects of a novel polysaccharide-based grinding aid (PGA) on magnetite's grinding and its reverse flotation. Batch grinding tests indicated that PGA improved grinding performance by reducing energy consumption, narrowing particle size distribution of products, and increasing their surface area compared to grinding without PGA. Flotation tests on pure samples illustrated that PGA has beneficial effects on magnetite depression (with negligible effect on quartz floatability) through reverse flotation separation. Flotation of the artificial mixture ground sample in the presence of PGA confirmed the benefits, giving a maximum Fe recovery and grade of 84.4 and 62.5%, respectively. In the absence of starch (depressant), PGA resulted in a separation efficiency of 56.1% compared to 43.7% without PGA. The PGA adsorption mechanism was mainly via physical interaction based on UV–vis spectra, zeta potential tests, Fourier transform infrared spectroscopy (FT-IR), and stability analyses. In general, the feasibility of using PGA, a natural green polymer, was beneficial for both grinding and reverse flotation separation performance.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Beneficial effects of a polysaccharide-based grinding aid on magnetite flotation: a green approach

Chipakwe

Karlkvist

Rosenkranz

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Fisetin-4′-O-α-D-glucopyranoside (FST-G1) with glucansucrase from L. mesenteroides NRRL B-1299CB4 exhibited greater water solubility and anti-inflammatory effects [ 13 ]. There are various types of glucan acceptors; of these, maltose is the most effective for glucan and oligosaccharide synthesis [ 14 ]. As research continues, the market demand for oligosaccharides continues to expand.…”

Section: Introductionmentioning

confidence: 99%

Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function

Gu,

Jiao,

Wang

et al. 2023

IJMS

View full text Add to dashboard Cite

This study explored the physicochemical properties and prebiotic activities of glucans and oligoglucans. Oligoglucans were obtained through the fermentation of Leuconostoc mesenteroides CICC6055 and the glucansucrase of strain CICC6055, while glucans were obtained only through fermentation. Thin-layer chromatography and high-performance liquid chromatography identified enzymatically synthesized oligoglucans with a higher yield. Differential scanning calorimetry and derivative thermogravimetry analyses revealed the heat resistance of the glucans and oligoglucans at 280–300 °C. Fourier transform-infrared spectroscopy and nuclear magnetic resonance analyses demonstrated that their main chains were linked with α-1,6-glycosidic bonds accompanied by glucose residue branching. In vitro fermentation experiments demonstrated that they not only improved the contents of short-chain fatty acids but also raised the abundance of predominant flora, such as Bacteroides, Firmicutes, Verrucomicrobia, and Proteobacteria. These results implicate glucansucrase as an efficacious tool for the enzyme synthesis of oligoglucans. Furthermore, both polysaccharides with different degrees of polymerization may be beneficial in maintaining a healthy human gut.

show abstract

“…The main structure of homopolymeric LAB EPSs is the glucan-type EPSs containing only glucose in the core repeating unit, and glucose units can be linked as (1 → 6), (1 → 2), (1 → 3) or (1 → 4)-linked α-D-glucose units. The type and proportion of these linkages in the glucan structure determine the glucan type, and so far, four glucans have been characterized as dextran, alternan, reuteran and mutan formed by mainly (1 → 6)-linked α-D-glucose units, alternating between (1 → 6)/(1 → 3)-linked α-D-glucose units, mainly (1 → 4)-linked α-D-glucose units and mainly (1 → 3)-linked α-D-glucose units [1][2][3][4]. Apart from glucans, LAB strains were reported to produce fructantype EPSs containing only fructose units in the EPS repeating unit structure [5].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Characterization of Dextran HE29 Produced by the Leuconostoc citreum HE29 Isolated from Traditional Fermented Pickle

İspirli

2023

Molecules

Self Cite

View full text Add to dashboard Cite

In this study, lactic acid bacteria (LAB) strains were isolated from traditional fermented pickles, and among the identified strains, Leuconostoc citreum HE29 with a strong slimy colony profile was further selected to determine the physicochemical and techno-functional properties of its exopolysaccharide (EPS). Glucose was the only sugar monomer in the core unit of EPS HE29 detected by HPLC analysis, and glucan HE29 revealed 7.3 kDa of molecular weight. Structural characterization of glucan HE29 by 1H and 13C NMR spectroscopy analysis demonstrated that EPS HE29 was a dextran-type EPS containing 5.3% levels of (1 → 3)-linked α-D-glucose units. This structural configuration was also supported by FT-IR analysis, which also demonstrated the functional groups within the dextran HE29 structure. In terms of thermal properties detected by TGA and DSC analysis, dextran HE29 demonstrated a degradation temperature of around 280 °C, showing its strong thermal features. A semi-crystalline nature was observed for dextran HE29 detected by XRD analysis. Finally, AFM and SEM analysis revealed tangled network-like properties and web-like branched structures for dextran HE29, respectively. These findings suggest the importance of plant-based fermented products as LAB sources in obtaining novel EPS structures with potential techno-functional roles.

show abstract

Characterization of a glucansucrase fromLactobacillus reuteriE81 and production of malto-oligosaccharides

Cited by 25 publications

References 35 publications

Beneficial effects of a polysaccharide-based grinding aid on magnetite flotation: a green approach

Beneficial effects of a polysaccharide-based grinding aid on magnetite flotation: a green approach

Glucans with Different Degrees of Polymerization from Leuconostoc mesenteroides CICC6055: Analysis of Physicochemical Properties and Intestinal Prebiotic Function

Physicochemical Characterization of Dextran HE29 Produced by the Leuconostoc citreum HE29 Isolated from Traditional Fermented Pickle

Contact Info

Product

Resources

About