Characterization of a high lipid-producing thermotolerant marine photosynthetic pico alga from genus Picochlorum (Trebouxiophyceae)

Mucko, Maja; Padisák, Judit; Udovič, Marija Gligora; Pálmai, Tamás; Novak, Tihana; Medić, Nikola; Gašparović, Blaženka; Štefanić, Petra Peharec; Orlić, Sandi; Ljubešić, Zrinka

doi:10.1080/09670262.2020.1757763

Cited by 12 publications

(10 citation statements)

References 88 publications

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“…The result showed that this method can extract 33.04% of total lipid from Picochlorum biomass. Furthermore, Mucko et al [83] isolated and characterized a high lipid-containing thermotolerant Picochlorum sp. PMFPPE4.…”

Section: Removal Of Nitrate and Phosphate By Picochlorum Sp In Differ...mentioning

confidence: 99%

Phycoremediation of nitrogen and phosphate from wastewater using Picochlorum sp.: A tenable approach

2021

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The wastewater originates from different industrial, municipal, and agriculture processes and contains different nitrogen sources, for example, nitrate, ammonium, nitrite, and phosphate such as inorganic and organic sources. The discharge of high nitrate and phosphate to the ecosystem or nearby water bodies can cause eutrophication which disbalances the aquatic ecosystem.Furthermore, ingestion of these pollutants can cause severe toxicity and disease to humans and animals. Thus, from an environmental and social perspective, its treatment is essential with no negative impact on the ecosystem. Microalgae are fundamental, mixotrophic microorganisms that treat different wastewater and utilize nitrate and phosphate in the medium as a source of nutrients. Among them, Picochlorum sp., have the potential to remove nitrogen and phosphate from wastewater. The biomass produced by Picochlorum sp. can be a promising candidate as a sustainable feedstock for biofuel and bioproducts formation. Thus, the present review provides a brief knowledge and understanding about the concentration of nitrogen and phosphate in different wastewater, their negative impacts, and the uptake mechanism of microalgae. Furthermore, the review also provides an insight into Picochlorum sp., and the effects of different physiological and nutritional factors on their growth, wastewater treatment efficacy, and biomass for value-added products and biorefinery applications. In addition, the review is useful to understand the potential of Picochlorum sp. for a tenable wastewater treatment process.

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Section: Removal Of Nitrate and Phosphate By Picochlorum Sp In Differ...mentioning

confidence: 99%

Phycoremediation of nitrogen and phosphate from wastewater using Picochlorum sp.: A tenable approach

2021

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“…A recent study reported a newly isolated green algal strain, Picochlorum sp. PMPFPPE4, that survived 1month incubation under total darkness, suggesting a potential capacity of mixotrophy (Mucko et al 2020). However, their study mainly focused on the biotechnological application, such as lipid production and thermotolerance; no verification of mixotrophy was conducted except speculating a possible osmotrophy (Mucko et al 2020).…”

Section: ) the Picochlorum Organisms Have Been Widelymentioning

confidence: 99%

“…Picochlorum (genus synonym "Nannochloris") is recognized as the most diverse and abundant green coccoid algae in the ocean (Tragin and Vaulot 2018). It has been widely studied with the aims of biotechnological application, such as oil production and halotolerance (Dahlin et al 2019, Mucko et al 2020. Nevertheless, there are no studies on the ecophysiological characteristics and the potential mixotrophic nutritional mode of Picochlorum sp.…”

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confidence: 99%

Evidence for mixotrophy in pico‐chlorophytes from a new Picochlorum (Trebouxiophyceae) strain

Pang

Liu

2021

Journal of Phycology

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Mixotrophs are increasingly recognized for their wide distribution in aquatic ecosystems and significant contributions to biogeochemical cycling. Many taxa within the phyla Chrysophyta, Cryptophyta, and Haptophyta are capable of phago-mixotrophy, however, phagotrophy in the Chlorophyta remains controversial due to insufficient research and solid evidence. In this study, we identified a new strain, Picochlorum sp. GLMF1 (Trebouxiophyceae), using 18S rRNA gene analysis and morphological observations. It displayed multi-cell division through autosporulation (two-or four-cell daughters) and has two unequal flagella that have never been reported in the genus Picochlorum. By using multiple methods, including 3D bioimaging analysis, acidic food vacuolelike compartment staining, and prey reduction calculation, we discovered and confirmed bacterivory in Picochlorum, which provided strong evidence for phago-mixotrophy in this green alga. In addition, we found that Picochlorum sp. GLMF1 cannot grow under complete darkness or prey-depleted conditions, suggesting that both light and bacteria are indispensable for this strain, and its mixotrophic nutrition mode is obligate. Like other phagophototrophs, Picochlorum sp. GLMF1 is capable of regulating their growth and ingestion rates according to light intensity and inorganic nutrient concentration. The confirmation of mixotrophy in this Picochlorum strain advances our understanding of the trophic roles of green algae, as well as the photosynthetic picoeukaryotes, in marine microbial food webs.

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“…Dangeard, Chlorella spp., Dunaliella spp., Prymnesium parvum Carter, Skeletonema costatutm (Gre ´ville) Cleve, Picochlorum spp. ), can produce, in a fast way, large amounts of hydrocarbons, especially lipids, which are suitable for biodiesel production (Razeghifard, 2013;Mucko et al, 2020). In this respect, we have to keep in mind that biofuel production from land plants (e.g., soybean, oil palm, sugarcane, wheat, maize) causes changes in the land use and a loss of biodiversity (Tudge et al, 2021).…”

Section: Fuel Productionmentioning

confidence: 99%

Ecosystem services provided by marine and freshwater phytoplankton

Naselli-Flores

Padisák

2022

Hydrobiologia

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Phytoplankton, the ecological group of microalgae adapted to live in apparent suspension in water masses, is much more than an ecosystem’s engineer. In this opinion paper, we use our experience as phytoplankton ecologists to list and highlight the services provided by phytoplankton, trying to demonstrate how their activity is fundamental to regulate and sustain Life on our Planet. Although the number of services produced by phytoplankton can be considered less numerous than that produced by other photosynthetic organisms, the ubiquity of this group of organisms, and their thriving across oceanic ecosystems make it one of the biological engines moving our biosphere. Supporting services provided by phytoplankton include almost half of the global primary and oxygen production. In addition, phytoplankton greatly pushes biogeochemical cycles and nutrient (re)cycling, not only in aquatic ecosystems but also in terrestrial ones. In addition, it significantly contributes to climate regulation (regulating services), supplies food, fuels, active ingredients and drugs, and genetic resources (provisioning services), has inspired artistic and craft works, mythology, and, of course, science (cultural services), and much more. Therefore, phytoplankton should be considered in all respects a true biosphere’s engineer.

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Characterization of a high lipid-producing thermotolerant marine photosynthetic pico alga from genus Picochlorum (Trebouxiophyceae)

Cited by 12 publications

References 88 publications

Phycoremediation of nitrogen and phosphate from wastewater using Picochlorum sp.: A tenable approach

Phycoremediation of nitrogen and phosphate from wastewater using Picochlorum sp.: A tenable approach

Evidence for mixotrophy in pico‐chlorophytes from a new Picochlorum (Trebouxiophyceae) strain

Ecosystem services provided by marine and freshwater phytoplankton

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