Characterization of a highly toxic, large molecular size heat‐stable enterotoxin produced by a clinical isolate of Yersinia enterocolitica

Y. enterocolitica and Y. pseudotuberculosis

“…In contrast to YSTa and YSTb, the mature form of YSTc was very long (53 AA), representing the largest molecular size toxin in the family of currently known STs. This secreted peptide was composed of the pro-and mature domain of the polypeptide (Yoshino et al, 1995b). The presence of the pro-domain in the secreted mature toxin suggests that in Y. enterocolitica, the proteolytic processing of the prodomain is not essential for the secretion of YSTc to the extracellular compartment through the outer membrane.…”

“…In contrast to YSTa, YSTb is produced in cells grown at 37°C (Yoshino et al, 1995b). YSTc was found to have an enterotoxigenic activity four times superior to those of YSTb and STh of E. coli, which had been known as the most potent STs.…”

“…YSTc Another novel heat-stable enterotoxin, designated "YSTc," was identified from an enterotoxigenic Y. enterocolitica O:3 strain, which failed to hybridize with ystA (Yoshino et al, 1995b). In contrast to YSTa and YSTb, the mature form of YSTc was very long (53 AA), representing the largest molecular size toxin in the family of currently known STs.…”

“…The presence of the pro-domain in the secreted mature toxin suggests that in Y. enterocolitica, the proteolytic processing of the prodomain is not essential for the secretion of YSTc to the extracellular compartment through the outer membrane. The toxic domain in the Cterminal portion of YSTc was well conserved (Yoshino et al, 1995b).…”

“…YSTc was found to have an enterotoxigenic activity four times superior to those of YSTb and STh of E. coli, which had been known as the most potent STs. Therefore, YSTc may be considered as the most potent enterotoxin in the ST family (Yoshino et al, 1995b).…”