Characterization of a<i>JAZ7</i>activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogen<i>Fusarium oxysporum</i>

Thatcher, Louise F.; Çevik, Volkan; Grant, Murray; Zhai, Bing; Jones, Jonathan D. G.; Manners, John M.; Kazan, Kemal

doi:10.1093/jxb/erw040

Cited by 76 publications

(68 citation statements)

References 87 publications

(194 reference statements)

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“…JAZs activities are tightly controlled at tissue levels. By constitutively overexpressing a JAZ repressor we may have disturbed that finely tuned JA homeostasis resulting in enhanced JA levels and signaling in OsJAZ9 overexpression plants as also reported earlier in Arabidopsis (Thatcher et al, 2016). Noticeably, JA levels were also enhanced in knockdown lines of AJAZ1 while remaining unchanged in the knockdown lines of NaZAZh (Li et al, 2017; Oh et al, 2012).…”

Section: Discussionsupporting

confidence: 61%

“…Further, the enhanced expression of JA biosynthesis and signaling genes like OsAOS2/3, OsLOX5/7/8, OsJAZOl and OsLEA3, OsVSP2 , respectively, confirms the higher JA signaling in OsJAZ9 OE plants during K deficiency. The enhanced JA signaling on overexpression of a repressor is surprising; however, similar observations on activated JA signaling were made on JAZ7 overexpression in Arabidopsis (Thatcher et al, 2016). In addition to K transporters, longer laterals might have also contributed to improved K uptake in OsJAZ9 OE plants.…”

Section: Discussionmentioning

confidence: 63%

“…However, elevated JA levels and JA signaling in OsJAZ9 OE lines are very intriguing. We suspect here a negative feedback regulation of JA homeostasis wherein constitutive overexpression of a JAZ repressor hyperactivated JA signaling (Chini et al, 2007; Thatcher et al, 2016). JAZs activities are tightly controlled at tissue levels.…”

Section: Discussionmentioning

confidence: 96%

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OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

Pandey

Mehra

et al. 2018

Preprint

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Potassium (K) which makes around 2-10% of plants total dry biomass, when become deficient, makes the plants highly susceptible to both abiotic and biotic stresses. Recent evidences suggest overlapping transcriptional responses to K deficiency and Jasmonate (JA) treatment in plants. However, a link between these responses was missing. Notably, K deficiency and JA application produce similar phenotypic and transcriptional responses. Here, we used molecular, physiological and morphological studies to analyze the role of OsJAZ9 in JA homeostasis, K deficiency and sheath blight resistance. We raised OsJAZ9 overexpression, knockdown, translational reporter and C-terminal deleted translational reporter lines in rice to establish the role of JA signaling in K ion homeostasis and OsJAZ9 as a critical component of JA signaling for K deficiency response. OsJAZ9 overexpression and knockdown provide K deficiency tolerance and sensitivity, respectively, by modulating various K transporters and root system architecture. Furthermore, RNA Seq and JA profiling revealed an elevation of JA responsive genes and JA levels in OsJAZ9 OE lines under K deficiency. Our data provide clear evidence on the crucial role of JAZ repressor, OsJAZ9 in improving K deficiency tolerance in rice by altering JA levels and signaling.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 63%

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OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

Pandey

Mehra

et al. 2018

Preprint

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“…Supernatant was immediately used in immunoprecipitation experiment or stored at −80°C until required (Hartmann et al ., ). GFP‐Trap (ChromoTek, Aachen, Germany) was used to immunoprecipitate the full‐length proteins as described by (Thatcher et al ., ). Isolation of full‐length proteins was confirmed by immunoblot analysis probing with rabbit anti‐GFP primary antibody (Thermo Fisher Scientific, San Jose, CA, USA) and goat‐anti‐rabbit IRDye 800CW (Li‐Cor Biosciences, Millenium Science, Mulgrave, VIC, Australia) to detect GFP using the Odyssey infrared imaging system (Li‐Cor Biosciences).…”

Section: Methodsmentioning

confidence: 97%

“…A . tumefaciens mediated transient expression experiments (method adapted from (Thatcher et al ., ) were performed on 4–5 weeks old Nicotiana benthamiana plants grown at 22°C under a 16‐h‐light/8‐h‐dark photoperiod. A. tumefaciens strains grown in 2YT media where harvested by centrifugation and resuspended in infiltration media (10 m m MES pH 5.6; 10 m m MgCl 2 ; 150 μ m acetosyringone) to a final OD 600 of 0.5 and incubated at room temperature for 3 h. Immediately prior to infiltration, each strain was diluted in infiltration media to OD 600 of 0.2 and infiltrated into the underside of tobacco leaves using a needleless syringe.…”

Section: Methodsmentioning

confidence: 99%

The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP‐dependent downstream signaling

et al. 2017

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The brassinosteroid receptor brassinosteroid insensitive 1 (BRI1) is a member of the leucine-rich repeat receptor-like kinase family. The intracellular kinase domain of BRI1 is an active kinase and also encapsulates a guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that the recombinant cytoplasmic domain of BRI1 generates pmol amounts of cGMP per μg protein with a preference for magnesium over manganese as a co-factor. Importantly, a functional BRI1 kinase is essential for optimal cGMP generation. Therefore, the guanylate cyclase activity of BRI1 is modulated by the kinase while cGMP, the product of the guanylate cyclase, in turn inhibits BRI1 kinase activity. Furthermore, we show using Arabidopsis root cell cultures that cGMP rapidly potentiates phosphorylation of the downstream substrate brassinosteroid signaling kinase 1 (BSK1). Taken together, our results suggest that cGMP acts as a modulator that enhances downstream signaling while dampening signal generation from the receptor.

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Jasmonate induced alternative splicing responses in Arabidopsis

et al. 2020

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Jasmonate is an essential phytohormone regulating plant growth, development, and defense. Alternative splicing (AS) in jasmonate ZIM‐domain (JAZ) repressors is well‐characterized and plays an important role in jasmonate signaling regulation. However, it is unknown whether other genes in the jasmonate signaling pathway are regulated by AS. We explore the potential for AS regulation in three Arabidopsis genotypes (WT, jaz2, jaz7) in response to methyl jasmonate (MeJA) treatment with respect to: (a) differential AS, (b) differential miRNA targeted AS, and (c) AS isoforms with novel functions. AS events identified from transcriptomic data were validated with proteomic data. Protein interaction networks identified two genes, SKIP and ALY4 whose products have both DNA‐ and RNA‐binding affinities, as potential key regulators mediating jasmonate signaling and AS regulation. We observed cases where AS alone, or AS and transcriptional regulation together, can influence gene expression in response to MeJA. Twenty‐one genes contain predicted miRNA target sites subjected to AS, which implies that AS is coupled to miRNA regulation. We identified 30 cases where alternatively spliced isoforms may have novel functions. For example, AS of bHLH160 generates an isoform without a basic domain, which may convert it from an activator to a repressor. Our study identified potential key regulators in AS regulation of jasmonate signaling pathway. These findings highlight the importance of AS regulation in the jasmonate signaling pathway, both alone and in collaboration with other regulators. Significance statement By exploring alternative splicing, we demonstrate its regulation in the jasmonate signaling pathway alone or in collaboration with other posttranscriptional regulations such as nonsense and microRNA‐mediated decay. A signal transduction network model for alternative splicing in jasmonate signaling pathway was generated, contributing to our understanding for this important, prevalent, but relatively unexplored regulatory mechanism in plants.

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Characterization of aJAZ7activation-tagged Arabidopsis mutant with increased susceptibility to the fungal pathogenFusarium oxysporum

Abstract: HighlightA JAZ7 T-DNA activation mutant confers increased JA-sensitivity, up-regulated defense and JA-mediated gene expression, and increased susceptibility to two pathogens that disrupt host JA-responses, Fusarium oxysporum and Pst DC3000.

Cited by 76 publications

References 87 publications

OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

OsJAZ9 overexpression improves potassium deficiency tolerance in rice by modulating jasmonic acid levels and signaling

The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP‐dependent downstream signaling

Jasmonate induced alternative splicing responses in Arabidopsis

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