Characterization of a <i>Vibrio cholerae</i> phage isolated from the coastal water of Peru

Talledo, Miguel; Rivera, Irma Nelly Gutierrez; Lipp, Erin K.; Neale, Angela; Karaolis, David K.R.; Huq, Anwar; Colwell, Rita R.

doi:10.1046/j.1462-2920.2003.00411.x

Cited by 11 publications

(18 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three vibriophages, AS 1 , AS 2 , and AS 3 were isolated from the sewage and pond waters of the outskirts of Kolkata, phages AS 1 and AS 2 had hexagonal heads and contractile tails and were of the family of Myoviridae [4]. Other studies have showed that a V. cholerae bacteriophages isolated from the sea water collected from the coastal water of Lima, Peru, belonged to the family of Myoviridae [17]. …”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Vibrio cholerae Bacteriophages Isolated from the Environmental Waters of the Lake Victoria Region of Kenya

et al. 2013

View full text Add to dashboard Cite

Over the last decade, cholera outbreaks have become common in some parts of Kenya. The most recent cholera outbreak occurred in Coastal and Lake Victoria region during January 2009 and May 2010, where a total of 11,769 cases and 274 deaths were reported by the Ministry of Public Health and Sanitation. The objective of this study is to isolate Vibriocholerae bacteriophages from the environmental waters of the Lake Victoria region of Kenya with potential for use as a biocontrol for cholera outbreaks. Water samples from wells, ponds, sewage effluent, boreholes, rivers, and lakes of the Lake Victoria region of Kenya were enriched for 48 h at 37 °C in broth containing a an environmental strain of V.cholerae. Bacteriophages were isolated from 5 out of the 42 environmental water samples taken. Isolated phages produced tiny, round, and clear plaques suggesting that these phages were lytic to V. cholerae. Transmission electron microscope examination revealed that all the nine phages belonged to the family Myoviridae, with typical icosahedral heads, long contractile tails, and fibers. Head had an average diameter of 88.3 nm and tail of length and width 84.9 and 16.1 nm, respectively. Vibriophages isolated from the Lake Victoria region of Kenya have been characterized and the isolated phages may have a potential to be used as antibacterial agents to control pathogenic V.cholerae bacteria in water reservoirs.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Phages infecting V. cholerae are well documented with earliest records of isolation of bacteriophages appearing in 1920s [17]. In V. cholerae bacteriophages were first reported by Siddiqui and Bhattacharyya [13].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Vibrio cholerae Bacteriophages Isolated from the Environmental Waters of the Lake Victoria Region of Kenya

et al. 2013

View full text Add to dashboard Cite

show abstract

“…An increase in phage numbers in the environment was also correlated with an increase in V. cholerae lytic bacteriophage in patient stool samples, with one of the predominant bacteriophage species belonging to the Myoviridae family (Seed et al, 2011). Environmental surveys have detected Myoviridae in regions where cholera outbreaks have occurred, such as Peru (Talledo et al, 2003), Kolkata (Sen and Ghosh, 2005), and Kenya (Maina et al, 2013). Control of V. cholerae by phage is supported by a continuous culture experiment, which suggests that V. cholerae populations may be influenced by phage to a larger extent than by nutrient limitation (Wei et al, 2011).…”

Section: Top-down Control By Predatory Micrograzersmentioning

confidence: 99%

Environmental reservoirs and mechanisms of persistence of Vibrio cholerae

Lutz¹,

Erken²,

Noorian³

et al. 2013

Front. Microbiol.

228

185

View full text Add to dashboard Cite

It is now well accepted that Vibrio cholerae, the causative agent of the water-borne disease cholera, is acquired from environmental sources where it persists between outbreaks of the disease. Recent advances in molecular technology have demonstrated that this bacterium can be detected in areas where it has not previously been isolated, indicating a much broader, global distribution of this bacterium outside of endemic regions. The environmental persistence of V. cholerae in the aquatic environment can be attributed to multiple intra- and interspecific strategies such as responsive gene regulation and biofilm formation on biotic and abiotic surfaces, as well as interactions with a multitude of other organisms. This review will discuss some of the mechanisms that enable the persistence of this bacterium in the environment. In particular, we will discuss how V. cholerae can survive stressors such as starvation, temperature, and salinity fluctuations as well as how the organism persists under constant predation by heterotrophic protists.

show abstract

“…Morphological analysis by transmission electron microscopy showed that bacteriophage VPUSM 8 belongs to the Myoviridae family (1, 4), while the genome sequence suggests that it is a P2-like bacteriophage (5). The genome of VPUSM 8 was 34,145 bp, with a G+C content of 48.92% and 43 identified CDSs.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Lytic Bacteriophage VPUSM 8 against O1 El Tor Inaba Vibrio cholerae

et al. 2017

View full text Add to dashboard Cite

show abstract

Characterization of a Vibrio cholerae phage isolated from the coastal water of Peru

Cited by 11 publications

References 18 publications

Characterization of Vibrio cholerae Bacteriophages Isolated from the Environmental Waters of the Lake Victoria Region of Kenya

Characterization of Vibrio cholerae Bacteriophages Isolated from the Environmental Waters of the Lake Victoria Region of Kenya

Environmental reservoirs and mechanisms of persistence of Vibrio cholerae

Complete Genome Sequence of Lytic Bacteriophage VPUSM 8 against O1 El Tor Inaba Vibrio cholerae

Contact Info

Product

Resources

About