Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans

Zotchev, Sergey B.; Soldatova, L.I; Orekhov, Andrey V.; Schrempf, Hildgund

doi:10.1016/0923-2508(92)90071-u

Cited by 19 publications

(14 citation statements)

References 17 publications

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“…The newly constructed plasmids (Table 1) were transformed into S. lividuns 66 protoplasts in the presence of poly-(ethylenglycol) (Hopwood et al, 1985). Plasmid DNA was extracted from S. lividuns using a modified alkaline method (Zotchev et al, 1992). Plasmid and phage constructs were isolated as previously reported (Sambrook et al, 1989).…”

Section: Methodsmentioning

confidence: 99%

Specific Interaction of the Streptomyces Chitin‐Binding Protein Chb1 with α‐Chitin

Zeltiņš

Schrempp

1997

European Journal of Biochemistry

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Streptomyces olivaceoviridis secretes a so far unique protein of 18.7 kDa (CHB1) which lacks catalytic activity. It interacts highly specifically with a-chitin, but not with @-chitin, chitosan, or cellulose. Each of the five codons for tryptophan (Trp) in the chbl gene was replaced by those for leucine (Leu) or tyrosine (Tyr). Eight corresponding mutant proteins and the wild-type protein were purified to homogeneity and their binding capacity to a-chitin was determined. The relative affinities to anti-CHB1 antibodies, the kinetics of binding, the dissociation constants, circular dichroism, and fluorescence emission spectra for three mutant types were compared to the characteristics of CHB1. The presented data lead to the following conclusions.

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Section: Methodsmentioning

confidence: 99%

Specific Interaction of the Streptomyces Chitin‐Binding Protein Chb1 with α‐Chitin

Zeltiņš

Schrempp

1997

European Journal of Biochemistry

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“…The Streptomyces conjugative linear plasmids SLP2 and pBL1 can be transferred to different species (Chen et al, 1993b;Zotchev et al, 1992), and linear plasmids of related bacteria are transferred among different genera (Kalkus etal., 1990), They may have strayed even farther afield. The organization and nucleotide sequences of some Streptomyces antibiotic synthesis genes are similar to those of certain fungi (Hara et al, 1991).…”

Section: Origin Of Linear Dna In Bacteriamentioning

confidence: 99%

Linear plasmids and chromosomes in bacteria

Hinnebusch

Tilly

1993

Molecular Microbiology

163

126

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“…4 Since then, many giant linear plasmids have been isolated from antibiotic producers, for example, pSB1 from S. bambergiensis (moenomycin), 68 and pSA1 and pSA2 from S. avermitilis (avermectin). 69 However, their functions in antibiotic production have not been confirmed.…”

Section: Other Giant Linear Plasmidsmentioning

confidence: 99%

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

Kinashi

2010

J Antibiot

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Many giant linear plasmids have been isolated from Streptomyces by using pulsed-field gel electrophoresis and some of them were found to carry an antibiotic biosynthetic cluster(s); SCP1 carries biosynthetic genes for methylenomycin, pSLA2-L for lankacidin and lankamycin, and pKSL for lasalocid and echinomycin. Accumulated data suggest that giant linear plasmids have played critical roles in genome evolution and horizontal transfer of secondary metabolism. In this review, I summarize typical examples of giant linear plasmids whose involvement in antibiotic production has been studied in some detail, emphasizing their finding processes and interaction with the host chromosomes. A hypothesis on horizontal transfer of secondary metabolism involving giant linear plasmids is proposed at the end.

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Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans

Cited by 19 publications

References 17 publications

Specific Interaction of the Streptomyces Chitin‐Binding Protein Chb1 with α‐Chitin

Specific Interaction of the Streptomyces Chitin‐Binding Protein Chb1 with α‐Chitin

Linear plasmids and chromosomes in bacteria

Giant linear plasmids in Streptomyces: a treasure trove of antibiotic biosynthetic clusters

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