Characterization of a membrane protein (VP001L) from infectious spleen and kidney necrosis virus (ISKNV)

Xu, Xiaopeng; Lü, Jing; Lu, Qingxia; Zhong, Hui; Weng, Shaoping; He, Jianguo

doi:10.1007/s11262-007-0177-x

Cited by 5 publications

(4 citation statements)

References 56 publications

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“…ORF1L exhibited high homology to the amino acid sequences of ORF1L of ISKNV and OSGIV [14,15]. ORF1L of ISKNV, containing 10 ~ 11 putative transmembrane domains, is a membrane protein and is considered to serve as a model for analyzing the topology and roles of different hydrophobic regions in multi-transmembrane proteins [36]. …”

Section: Resultsmentioning

confidence: 99%

Complete genome sequence of a Megalocytivirus (family Iridoviridae) associated with turbot mortality in China

Shi

Jia

Yang

et al. 2010

Virol J

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BackgroundTurbot reddish body iridovirus (TRBIV) causes serious systemic diseases with high mortality in the cultured turbot, Scophthalmus maximus. We here sequenced and analyzed the complete genome of TRBIV, which was identified in Shandong province, China.ResultsThe genome of TRBIV is a linear double-stranded DNA of 110,104 base pairs, comprising 55% G + C. Total 115 open reading frames were identified, encoding polypeptides ranging from 40 to 1168 amino acids. Amino acid sequences analysis revealed that 39 of the 115 potential gene products of TRBIV show significant homology to other iridovirus proteins. Phylogenetic analysis of conserved genes indicated that TRBIV is closely related to infectious spleen and kidney necrosis virus (ISKNV), rock bream iridovirus (RBIV), orange-spotted grouper iridovirus (OSGIV), and large yellow croaker iridovirus (LYCIV). The results indicated that TRBIV belongs to the genus Megalocytivirus (family Iridoviridae).ConclusionsThe determination of the genome of TRBIV will provide useful information for comparative study of Megalocytivirus and developing strategies to control outbreaks of TRBIV-induced disease.

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Section: Resultsmentioning

confidence: 99%

Complete genome sequence of a Megalocytivirus (family Iridoviridae) associated with turbot mortality in China

Shi

Jia

Yang

et al. 2010

Virol J

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“…Further research is required to resolve this question. Recently, a number of studies have been undertaken to identify gene function in ISKNV [47,48,49,50,51]. These expanding biochemical studies will clarify the function of specific ISKNV genes and indicate whether megalocytiviruses display replicative strategies unique to this viral genus.…”

Section: Phylogenetic Analysis and Host Range Of Megalocytivirusesmentioning

confidence: 99%

Megalocytiviruses

Kurita

Nakajima

2012

Viruses

234

218

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The genus Megalocytivirus, represented by red sea bream iridovirus (RSIV), the first identified and one of the best characterized megalocytiviruses, Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus, and numerous other isolates, is the newest genus within the family Iridoviridae. Viruses within this genus are causative agents of severe disease accompanied by high mortality in multiple species of marine and freshwater fish. To date outbreaks of megalocytivirus-induced disease have occurred primarily in south-east Asia and Japan, but infections have been detected in Australia and North America following the importation of infected ornamental fish. The first outbreak of megalocytiviral disease was recorded in cultured red sea bream (Pagrus major) in Japan in 1990 and was designated red sea bream iridovirus disease (RSIVD). Following infection fish became lethargic and exhibited severe anemia, petechiae of the gills, and enlargement of the spleen. Although RSIV was identified as an iridovirus, sequence analyses of RSIV genes revealed that the virus did not belong to any of the four known genera within the family Iridoviridae. Thus a new, fifth genus was established and designated Megalocytivirus to reflect the characteristic presence of enlarged basophilic cells within infected organs. Indirect immunofluorescence tests employing recently generated monoclonal antibodies and PCR assays are currently used in the rapid diagnosis of RSIVD. For disease control, a formalin-killed vaccine was developed and is now commercially available in Japan for several fish species. Following the identification of RSIV, markedly similar viruses such as infectious spleen and kidney necrosis virus (ISKNV), dwarf gourami iridovirus (DGIV), turbot reddish body iridovirus (TRBIV), Taiwan grouper iridovirus (TGIV), and rock bream iridovirus (RBIV) were isolated in East and Southeast Asia. Phylogenetic analyses of the major capsid protein (MCP) and ATPase genes indicated that although these viruses shared considerable sequence identity, they could be divided into three tentative species, represented by RSIV, ISKNV and TRBIV, respectively. Whole genome analyses have been reported for several of these viruses. Sequence analysis detected a characteristic difference in the genetic composition of megalocytiviruses and other members of the family in reference to the large and small subunits of ribonucleotide reductase (RR-1, RR‑2). Megalocytiviruses contain only the RR-2 gene, which is of eukaryotic origin; whereas the other genera encode both the RR-1 and RR-2 genes which are thought to originate from Rickettsia-like α-proteobacteria.

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“…To better understand the molecular mechanism of iridovirus pathogenesis and explore the strategy for prevention and control iridovirus diseases, complete genomes of 19 iridovirus isolates were sequenced and annotated up to now [6-8]. Some functional genes encoded by individual iridovirus, such as infection cell protein (ICP), capsid protein, membrane protein and virus replication associated enzymes have been identified and characterized [9-11]. However, the function of many iridovirus core structural genes remained largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Characterization of an envelope gene VP19 from Singapore grouper iridovirus

Huang

Gong

Huang

et al. 2013

Virol J

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BackgroundViral envelope proteins are always proposed to exert important function during virus infection and replication. Vertebrate iridoviruses are enveloped large DNA virus, which can cause great economic losses in aquaculture and ecological destruction. Although numerous iridovirus envelope proteins have been identified using bioinformatics and proteomic methods, their roles in virus infection remained largely unknown.MethodsUsing SMART and TMHMM programs, we investigated the structural characteristics of Singapore grouper iridovirus (SGIV) VP19. A specific antibody against VP19 was generated and the expression profile of VP19 was clarified. The subcellular localization of VP19 in the absence or presence of other viral products was determined via transfection and immune fluorescence assay. In addition, Western blot assay and electron microscopy examination were performed to demonstrate whether SGIV VP19 was an envelope protein or a capsid protein.ResultsHere, SGIV VP19 was cloned and characterized. Among all sequenced iridoviruses, VP19 and its orthologues shared common features, including 19 invariant cysteines, a proline-rich motif and a predicted transmembrane domain. Subsequently, the protein synthesis of VP19 was only detected at the late stage of SGIV infection and inhibited obviously by treating with AraC, confirming that VP19 was a late expressed protein. Ectopic expression of EGFP-VP19 in vitro displayed a punctate pattern in the cytoplasm. In SGIV infected cells, the newly synthesized VP19 protein was initially localized in the cytoplasm in a punctate pattern, and then aggregated into the virus assembly site at the late stage of SGIV infection, suggesting that other viral protein products were essential for VP19’s function during SGIV infection. In addition, Western blot assay and electron microscopy observation revealed that SGIV VP19 was associated with viral envelope, which was different from major capsid protein (MCP).ConclusionTaken together, the current data suggested that VP19 represented a conserved envelope protein in iridovirus, and might contribute greatly to virus assembly during virus infection.

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Characterization of a membrane protein (VP001L) from infectious spleen and kidney necrosis virus (ISKNV)

Cited by 5 publications

References 56 publications

Complete genome sequence of a Megalocytivirus (family Iridoviridae) associated with turbot mortality in China

Complete genome sequence of a Megalocytivirus (family Iridoviridae) associated with turbot mortality in China

Megalocytiviruses

Characterization of an envelope gene VP19 from Singapore grouper iridovirus

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