Characterization of a Microsphere Formulation Containing Glucose Oxidase and its In Vivo Efficacy in a Murine Solid Tumor Model

Abstract

The results suggest that the ACMS-GOX formulation has the potential for the intratumoral delivery of therapeutic proteins to treat solid tumors.

In Situ Growth of a Cationic Polymer from the N-Terminus of Glucose Oxidase To Regulate H2O2Generation for Cancer Starvation and H2O2Therapy

“…However, it is difficult to modulate H 2 O 2 generation after intratumoral administration, which may lead to limited antitumor efficacy and serious systemic side effects. To address this challenge, several GOX delivery systems, such as PEGylated GOX, 11 polystyrene microsphere-conjugated GOX, 12 alginate/chitosan microsphere-conjugated GOX, 21 and GOX−polymer nanogels, 22 have been developed to prolong the tumor retention of GOX post intratumoral injection via the mechanisms of increasing the size and stability of GOX. Nevertheless, these strategies did not show dramatically improved antitumor efficacy with considerably enhanced tolerability and significantly reduced side effects.…”

Self-Organization of Glucose Oxidase–Polymer Surfactant Nanoconstructs in Solvent-Free Soft Solids and Liquids

“…The enzyme is then regenerated often in association with the reduction of dioxygen to hydrogen peroxide. Interestingly, differential scanning calorimetry (DSC) experiments performed on dry powders of GOx have shown a first-order endothermic transition at temperatures between 50 and 60 °C, which was described as a glass transition temperature ( T g ) arising from a change in the intramolecular dynamics of the polypeptide chain. − Strictly speaking, T g for small molecules or synthetic polymers describes a second-order transition associated with a change in the heat capacity of a material as an amorphous glass turns into a rubbery solid, and accordingly, this transition will be described herein as a conformation transition temperature ( T c ).…”

Effect of formulation factors on the bioactivity of glucose oxidase encapsulated chitosan–alginate microspheres: In vitro investigation and mathematical model prediction

“…However, the low in vivo stability of native enzymes limited their application. Therefore, modification of the enzymes using polymer conjugations or microencapsulation has been pursued with improved stability and prolonged activity (Averill-Bates et al, 2005;Demers et al, 2001;Liu et al, 2007Liu et al, , 2009Liu et al, , 2010. Chitosan coated alginate-calcium microspheres with a matrix structure have been developed in our group to encapsulate glucose oxidase (GOX) and demonstrated to be effective in killing P-glycoprotein overexpressing cancer cells in vitro and inhibiting tumor growth in vivo (Liu et al, 2007.…”