Characterization of a molt-related myostatin gene ( FmMstn ) from the banana shrimp Fenneropenaeus merguiensis

Zhuo, Rui; Zhou, Ting; Yang, Shi Ping; Chan, S. C.

doi:10.1016/j.ygcen.2017.03.010

Cited by 39 publications

(22 citation statements)

References 39 publications

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“…To examine the expression dynamics of Mr‐Dsx in male individuals during the moulting cycle, prawns at different moulting stages were collected and three types of tissues, including the testis, AG and eyestalk, were dissected from these animals. The moulting stages, including early post‐moulting stage (A), late post‐moulting stage (B), inter‐moulting stage (C1, C2, C3) and pre‐moulting stage (D0, D1, D2), were determined based on microscopic observation of its pleopod according to the methods in previous studies (Wang et al, ; Zhuo, Zhou, Yang, & Chan, ). To investigate the impact of eyestalk‐derived neuropeptides on Mr‐Dsx expression, 10 male prawns were subjected to UEA and another 10 male prawns maintained under normal conditions were used as control.…”

Section: Methodsmentioning

confidence: 99%

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

et al. 2019

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Macrobrachium rosenbergii is an important aquaculture species known as the king of freshwater prawn. Macrobrachium rosenbergii displays remarkable sexual dimorphism, yet our knowledge of the molecular mechanism underpinning sex determination and differentiation of M. rosenbergii is still fragmentary. In this study, through in silico prediction and experimental characterization, we identified a new Dmrt gene (Mr-Dsx) from M. rosenbergii. The cDNA of Mr-Dsx included a 115 bp 5′ untranslated region (UTR), a 2,568 bp open reading frame (ORF) and a 259 bp 3′ UTR. Mr-Dsx was expressed in a wide range of tissues, and showed dynamic expression changes at different moulting stages in the testis, androgenic gland (AG) and eyestalk. In response to unilateral eyestalk ablation (UEA), Mr-Dsx was significantly induced in the testis and AG. Gene knockdown of Mr-Dsx resulted in pronounced suppression of the insulin-like androgenic hormone (IAG) gene. The results suggest that Mr-Dsx is likely to be negatively regulated by the eyestalk neurohormones, and Mr-Dsx may participate in the transcriptional activation of IAG. The study characterized the potential roles of a newly identified Dmrt homologue from M. rosenbergii, and would contribute to a deeper understanding of the molecular regulatory network underlying sex differentiation and moulting of prawns. K E Y W O R D S crustacean, Dmrt, Macrobrachium rosenbergii, moulting, sexual differentiation

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Section: Methodsmentioning

confidence: 99%

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

et al. 2019

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“…PCR products were purified using a gel extraction kit (Sangon, Shanghai, China), followed by sequencing using an ABI3730 DNA analyzer. The 5ʹ- promoter region of MnLDH was cloned using a genome walking kit (TaKaRa, Dalian, China) with primers GSPR1, GSPR2, GSPR3 by previous methods [ 43 ]. A 2017-bp DNA fragment was obtained and cloned into vector pMD18-T (Takara, Dalian, China) for sequencing.…”

Section: Methodsmentioning

confidence: 99%

Molecular Cloning and Expression Analysis of Lactate Dehydrogenase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia

Sun

Zhu

et al. 2018

IJMS

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Metabolic adaption to hypoxic stress in crustaceans implies a shift from aerobic to anaerobic metabolism. Lactate dehydrogenase (LDH) is a key enzyme in glycolysis in prawns. However, very little is known about the role of LDH in hypoxia inducible factor (HIF) pathways of prawns. In this study, full-length cDNA of LDH (MnLDH) was obtained from the oriental river prawn Macrobrachium nipponense, and was characterized. The full-length cDNA is 2267-bp with an open reading frame of 999 bp coding for a protein of 333 amino acids with conserved domains important for function and regulation. Phylogenetic analysis showed that MnLDH is close to LDHs from other invertebrates. Quantitative real-time PCR revealed that MnLDH is expressed in various tissues with the highest expression level in muscle. MnLDH mRNA transcript and protein abundance in muscle, but not in hepatopancreas, were induced by hypoxia. Silencing of hypoxia-inducible factor 1 (HIF-1) α or HIF-1β subunits blocked the hypoxia-dependent increase of LDH expression and enzyme activity in muscle. A series of MnLDH promoter sequences, especially the full-length promoter, generated an increase in luciferase expression relative to promoterless vector; furthermore, the expression of luciferase was induced by hypoxia. These results demonstrate that MnLDH is probably involved a HIF-1-dependent pathway during hypoxia in the highly active metabolism of muscle.

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“…Likewise, a separate study on shrimp revealed that silencing of the Mstn/Gdf11 gene by tail-muscle injection of dsRNA significantly diminished growth and the survival rate 15 , indicating that ancestral MSTN/GDF11 in invertebrates is a positive regulator of growth and development, unlike vertebrate MSTN. Moreover, Zhuo et al 47 identified long (428 amino acids) and short (420 amino acids) forms of banana shrimp MSTN and FmMSTN, and the long form was positively correlated with a larger size in shrimp. Injection of dsRNA targeting FmMstn into these shrimp impaired their normal molting cycle but also resulted in the enlargement of the pleopod muscles 47 , which contradicts earlier findings.…”

Section: Functions Of Mstn/gdf11 In Invertebratesmentioning

confidence: 99%

“…Moreover, Zhuo et al 47 identified long (428 amino acids) and short (420 amino acids) forms of banana shrimp MSTN and FmMSTN, and the long form was positively correlated with a larger size in shrimp. Injection of dsRNA targeting FmMstn into these shrimp impaired their normal molting cycle but also resulted in the enlargement of the pleopod muscles 47 , which contradicts earlier findings. Further analysis and quantitation of the muscle fiber size in different muscle types are required to fully elucidate the effects of shrimp MSTN/ GDF11 on controlling muscle development.…”

Section: Functions Of Mstn/gdf11 In Invertebratesmentioning

confidence: 99%

Similar sequences but dissimilar biological functions of GDF11 and myostatin

Suh

Lee

2020

Exp Mol Med

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Growth differentiation factor 11 (GDF11) and myostatin (MSTN) are closely related TGFβ family members that are often believed to serve similar functions due to their high homology. However, genetic studies in animals provide clear evidence that they perform distinct roles. While the loss of Mstn leads to hypermuscularity, the deletion of Gdf11 results in abnormal skeletal patterning and organ development. The perinatal lethality of Gdf11-null mice, which contrasts with the long-term viability of Mstn-null mice, has led most research to focus on utilizing recombinant GDF11 proteins to investigate the postnatal functions of GDF11. However, the reported outcomes of the exogenous application of recombinant GDF11 proteins are controversial partly because of the different sources and qualities of recombinant GDF11 used and because recombinant GDF11 and MSTN proteins are nearly indistinguishable due to their similar structural and biochemical properties. Here, we analyze the similarities and differences between GDF11 and MSTN from an evolutionary point of view and summarize the current understanding of the biological processing, signaling, and physiological functions of GDF11 and MSTN. Finally, we discuss the potential use of recombinant GDF11 as a therapeutic option for a wide range of medical conditions and the possible adverse effects of GDF11 inhibition mediated by MSTN inhibitors.

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Characterization of a molt-related myostatin gene ( FmMstn ) from the banana shrimp Fenneropenaeus merguiensis

Cited by 39 publications

References 39 publications

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

Potential involvement of a DMRT family member ( Mr‐Dsx ) in the regulation of sexual differentiation and moulting in the giant river prawn Macrobrachium rosenbergii

Molecular Cloning and Expression Analysis of Lactate Dehydrogenase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia

Similar sequences but dissimilar biological functions of GDF11 and myostatin

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