The aim of this study was to investigate the antimicrobial and cytotoxic potential of Streptomyces sp. isolated from carbonate rock in the Amazon. The antimicrobial activity was verified by the diffusion technique in agar against pathogens such as Micrococcus lutteus ATCC 7468, Streptococcus pneumoniae ATCC700699; Proteus vulgaris ATCC 13315, Escherichia coli ATCC 10536, Acinetobacter baumanii ATCC 19606.Cytotoxic activity was investigated by the inhibition of cell growth (%) of samples tested in human colorectal carcinoma cell line (HCT116), at a concentration of 10 µg/mL (mean ± SEM; n = 2). The isolate showed a broad spectrum of antimicrobial activity against test microorganisms. This isolate showed maximum antibacterial activity against Escherichia coli ATCC 10536 (13 ± 0.0 mm) and Proteus vulgaris ATCC 13315 (12.8 ± 0.29 mm) and the most resistant microorganism against antibacterial activity of this isolate was Acinetobacter baumanii ATCC 19606 (11.5 ± 1.15 mm). This metabolites was extracted by solid:liquid method with ethyl acetate organic solvent. The percentage of inhibition of cell growth of the crude extract tested in a human colorectal carcinoma line (HCT116) was 96,55%. The determination of antioxidant activity by capturing the free radical ABTS had an inhibition of 35.60 ± 2.01%, while the antioxidant potential by capturing the free radical DPPH had an inhibition of 10.65 ± 1.95%. The LC-MS analysis showed the presence of four peaks, where it was identified that the absorbance of substances 5,791 and 13,556 were similar to 9,921 and 17,077, respectively; this suggests the possibility of PML 5 having a metabolic pathway synthesizing substances of the same class. The CG-MS of the extract showed the presence of 10 volatile compounds. The main compound was Cyclododecane (83.75%) compared to other bioactive compounds. These results reinforce the potential of Amazonian biodiversity, revealing that actinobacteria produce antimicrobial and cytotoxic bioactive substances of interest to the pharmaceutical industry.