Characterization of a new alginate lyase from newly isolated <i>Flavobacterium</i> sp. S20

Huang, Liejun; Zhou, Jungang; Xiao, Li; Peng, Qiang; Lü, Hong; Du, Yuguang

doi:10.1007/s10295-012-1210-1

Cited by 101 publications

(52 citation statements)

References 39 publications

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“…13 A number of alginate lyases have been identified, cloned, purified and characterized from various sources, [14][15][16][17][18] including marine and terrestrial bacteria, marine mollusks and algae. They can be classed into 2 groups due to their substrate specificities, 13 molecular weights 19 and action modes.…”

Section: Introductionmentioning

confidence: 99%

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

2015

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A lginate lyases catalyze the degradation of alginate, a complex copolymer of a-L-guluronate and its C5 epimer b-D-mannuronate. The enzymes have been isolated from various kinds of organisms with different substrate specificities, including algae, marine mollusks, marine and terrestrial bacteria, and some viruses and fungi. With the progress of structural biology, many kinds of alginate lyases of different polysaccharide lyases families have been characterized by obtaining crystal structures, and the catalytic mechanism has also been elucidated. Combined with various studies, we summarized the source, classification and properties of the alginate lyases from different polysaccharide lyases families. The relationship between substrate specificity and protein sequence was also investigated.

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Section: Introductionmentioning

confidence: 99%

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

2015

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“…3B). algL activity has been determined by various methods [1,15,24,25] . This enzyme depolymerizes alginate by β-elimination reaction to produce 4 deoxy-erytrohex-4-enopyranuronosyl groups at non-reducing end [1] .…”

Section: Resultsmentioning

confidence: 99%

Screening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production

Tavafi¹,

Ali²,

Ghadam³

et al. 2017

IBJ

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Background: Alginate is a linear polysaccharide consisting of guluronate (polyG) and mannuronate (polyM) subunits. Methods: In the initial screening of alginate-degrading bacteria from soil, 10 isolates were able to grow on minimal medium containing alginate. The optimization of cell growth and alginate lyase (algL) production was carried out by the addition of 0.8% alginate and 0.2-0.3 M NaCl to the culture medium. Of 10 isolates, one was selected based on its fast growth rate on minimal 9 medium containing 0.4% sodium alginate. The selected bacterium, identified based on morphological and biochemical characteristics, as well as 16S rDNA sequence data, was confirmed to be an isolate belonging to the genus Bacillus and designated as Bacillus sp. TAG8. Results: The results showed the ability of Bacillus sp. TAG8 in utilizing alginate as a sole carbon source. Bacillus sp. TAG8 growth and algL production were augmented with an increase in sodium alginate concentration and also by the addition of 0.2-0.3 M NaCl. Molecular analysis of TAG8 algL gene showed 99% sequence identity with algL of Pseudomonas aeruginosa PAO1. The algL produced by Bacillus sp. TAG8 cleaved both polyM and polyG blocks in alginate molecule, as well as acetylated alginate residues, confirming the bifunctionality of the isolated lyase. Conclusion: The identification of novel algL genes from microbial communities constitutes a new approach for exploring lyases with specific activity against bacterial alginates and may thus contribute to the eradication of persistent biofilms from clinical samples.

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“…Enhancement of the nutritive value of L. japonica feedstuff via fermentation could increase the bio-availability of nutrients, reduce or remove the component that is difficult to utilize or to convert it into a more readily utilized form. Most of the previous studies on algin degradation have focused on the isolation and purification of alginate lyase for commercial purposes [26,27,28,29,30]. Alginate lyase is induced when algin-degrading bacteria are grown in the presence of algin [8].…”

Section: Discussionmentioning

confidence: 99%

Response surface methodology based optimization for degradation of align in Laminaria japonica feedstuff via fermentation by Bacillus in Apostichopus japonicas farming

Wang

et al. 2016

Electronic Journal of Biotechnology

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a b s t r a c tBackground: The alga Laminaria japonica is the most economically important brown seaweed cultured in China, which is used as food and aquatic animal feedstuff. However, the use of L. japonica as a feedstuff in Apostichopus japonicas farming is not ideal because A. japonicas does not produce enough enzyme activity for degrading the large amount of algin present in L. japonica. In this study, semi solid fermentation of the L. japonica feedstuff employing a Bacillus strain as the microbe was used to as a mean to degrade the algin content in L. japonica feedstuff. Results: The Bacillus strain, Bacillus amyloliquefaciens WB1, was isolated by virtue of its ability to utilize sodium alginate as the sole carbon source. Eight factors affecting growth and algin-degrading capacity of WB1 were investigated. The results of Plackett-Burman design indicated that fermentation time, beef extract, and solvent to solid ratio were the significant parameters. Furthermore, the mutual interaction between the solvent to solid ratio and beef extract concentration was more significant than the other pairs of parameters on algin degradation. Optimal values obtained from Central-Composite Design were 113.94 h for fermentation time, 0.3% (w/v) beef extract and 44.87 (v/w) ratio of solvent to feedstuff. Under optimal conditions, 56.88% of the algin was degraded when a 50-fold scale-up fermentation was carried out, using a 5-L fermenter. Conclusions: This study provides an alternative and economical way to reduce the algin content in L. japonica through degradation by WB1, making it a promising potential source of feed for cultured L. japonica.

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Characterization of a new alginate lyase from newly isolated Flavobacterium sp. S20

Cited by 101 publications

References 39 publications

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

Alginate lyase: Review of major sources and classification, properties, structure-function analysis and applications

Screening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production

Response surface methodology based optimization for degradation of align in Laminaria japonica feedstuff via fermentation by Bacillus in Apostichopus japonicas farming

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