Characterization of a new antifungal lipid transfer protein from wheat

Kirubakaran, S. Isaac; Begum, Shahnaz; Ulaganathan, Kandasamy; Sakthivel, Natarajan

doi:10.1016/j.plaphy.2008.05.007

Cited by 80 publications

(43 citation statements)

References 32 publications

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“…Exogenous application of a VvLTP4-JA complex to grapevine plantlets also induced a high level of tolerance against B. cinerea (Girault et al, 2008). The recombinant wheat LTP 3F1 exhibited broad-spectrum antifungal activity against Alternaria sp., Rhizoctonia solani, Curvularia lunata, Bipolaris oryzae, Cylindrocladium scoparium, B. cinerea, and Sarocladium oryzae (Kirubakaran et al, 2008). LTPs were also reported to be involved in long-distance signaling during systemically acquired resistance in Arabidopsis and in cell wall loosening during pollen-pistil interactions (Nieuwland et al, 2005), and the anti-phytopathogenic activity of LTPs probably involves membrane permeabilization of target cells (Regente et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Expression of recombinant EARLI1, a hybrid proline-rich protein of Arabidopsis, in Escherichia coli and its inhibition effect to the growth of fungal pathogens and Saccharomyces cerevisiae

Zhang

et al. 2012

Gene

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EARLI1 is an Arabidopsis gene with pleiotropic effects previously shown to have auxiliary functions in protecting plants against freezing-induced cellular damage and promoting germinability under low-temperature and salinity stresses. Here we determined whether recombinant EARLI1 protein has anti-fungal activity. Recombinant EARLI1 protein lacking its signal peptide was produced in Escherichia coli BL21(DE3) using isopropyl β-d-1-thiogalactopyranoside (IPTG) induction and the prokaryotic expression vector pET28a. Expression of EARLI1 was analyzed by Western blotting and the protein was purified using affinity chromatography. Recombinant EARLI1 protein was applied to fungal cultures of Saccharomyces cerevisiae, Botrytis cinerea and Fusarium oxysporum, and membrane permeability was determined using SYTOX green. Full-length EARLI1 was expressed in S. cerevisiae from the GAL1 promoter using 2% galactose and yeast cell viability was compared to control cells. Our results indicated that application of recombinant EARLI1 protein to B. cinerea and F. oxysporum could inhibit the growth of the necrotrophic fungi. Besides, addition of the recombinant protein to liquid cultures of S. cerevisiae significantly suppressed yeast growth and cell viability by increasing membrane permeability, and in vivo expression of the secreted form of EARLI1 in S. cerevisiae also had a remarkable inhibition effect on the growth of yeast cells.

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Section: Discussionmentioning

confidence: 99%

Expression of recombinant EARLI1, a hybrid proline-rich protein of Arabidopsis, in Escherichia coli and its inhibition effect to the growth of fungal pathogens and Saccharomyces cerevisiae

Zhang

et al. 2012

Gene

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“…The fungus Gibberella fujikuroi was kindly provided by Dr. B. Fraga, Natural Products and Agrobiology Institute, CSIC, Canary Islands, Spain. The agar dilution technique was used to evaluate the effect of the prenylated compounds on the growth of P. cinnamomi 41,42 , G. fujikuroi 43 and B. cinerea 44,45 . Fungicidal activity assays of these compounds were performed in microcultures by growing the fungi in sterile Petri dishes (35 mm) at a final volume of 2 mL medium containing different compound concentrations.…”

Section: In Vitro Fungicidal Activity Assaysmentioning

confidence: 99%

Synthesis, Anti-Phytopathogenic and DPPH Radical Scavenging Activities of C-Prenylated Acetophenones and Benzaldehydes

Osorio

Quiroz

Carvajal

et al. 2016

J. Chil. Chem. Soc.

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The syntheses of six prenylated acetophenone and benzaldehyde derivatives and their anti-phytopathogenic and antioxidant activities are reported. These compounds were obtained by electrophilic aromatic substitution (S E Ar) of the corresponding arenes and 3-methyl-2-buten-1-ol using ZnCl 2 as a Lewis acid catalyst in ethyl acetate. Reasonable to good yields were obtained based on unrecovered aromatic starting material (45-73%). All the synthesized compounds were evaluated against phytopathogenic gram-negative bacteria Agrobacterium tumefaciens, Pseudomonas syringae and Erwinia carotovora and plant fungal pathogens Botrytis cinerea, Phytophthora cinnamomi and Gibberella fujikuroi. The antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and expressed as half maximal inhibitory concentration (IC 50 ) values in µM concentrations. The antioxidant activity went from 27.20 µM to >100 µM. Compound 11 showed statistically significant inhibition of the growth of Botrytis cinerea, and compounds 13 and 15 showed statistically significant inhibition of the growth of Phytophthora cinnamomi, with respect to negative control fungal growth. All six compounds showed bacteriostatic effects against gram-negative plant pathogenic bacteria with IC 50 values between 250 and <3.9 µM.

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“…The nsLTPs have been found in many different land plants, including Arabidopsis, rice, wheat, pine, peach and corn Boutrot et al, 2008;Jang et al, 2008;Kirubakaran et al, 2008;Kovaleva et al, 2009). The protein family is very large, with more than 150 members in wheat, 49 in Arabidopsis and 52 in rice (Boutrot et al, 2008).…”

Section: The Non-specific Lipid Transfer Proteins Backgroundmentioning

confidence: 99%

Plant lipid transfer proteins: Evolution, expression and function

Edstam¹

2013

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Characterization of a new antifungal lipid transfer protein from wheat

Cited by 80 publications

References 32 publications

Expression of recombinant EARLI1, a hybrid proline-rich protein of Arabidopsis, in Escherichia coli and its inhibition effect to the growth of fungal pathogens and Saccharomyces cerevisiae

Expression of recombinant EARLI1, a hybrid proline-rich protein of Arabidopsis, in Escherichia coli and its inhibition effect to the growth of fungal pathogens and Saccharomyces cerevisiae

Synthesis, Anti-Phytopathogenic and DPPH Radical Scavenging Activities of C-Prenylated Acetophenones and Benzaldehydes

Plant lipid transfer proteins: Evolution, expression and function

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