Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus

Yashwanth, B. S.; Goswami, Mukunda; Valappil, Rajendran Kooloth; Thakuria, Dimpal; Chaudhari, Aparna

doi:10.1038/s41598-020-76807-7

Cited by 27 publications

(14 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…27,47 cryopreservation was demonstrated, and found to be higher than 80%, which is consistent with previous studies. 15,34,36 The cell viability obtained in the present study was similar to that reported by Ishak Ahmed et al 37 (85-90% recovery).…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

The Development and Evaluation of Brain and Heart Cell Lines from a Marine Fish for Use in Xenobiotic-Induced Cytotoxicity Testing

2021

View full text Add to dashboard Cite

Two cell lines derived from the brain and heart of a Pacific white snook specimen ( Centropomus viridis) were developed and evaluated in terms of their responsiveness to glyphosate-induced cytotoxicity. The cells were grown in Leibovitz-15 (L-15) medium supplemented with 10% fetal bovine serum (FBS) and were passaged 36 times. Growth was tested at different concentrations of FBS (5, 10 and 20%) at 27°C. The cell lines were cryopreserved at different passages and were successfully thawed, with a survival rate greater than 80% without detectable contamination. At passage 36, the cells were used to assess the deleterious effects of glyphosate, and cell proliferation was measured by direct counting and with the MTT assay. Similar LC50 values were obtained with both methods. Although the principles behind these two assessment methods differ, our results show that both are suitable for evaluating glyphosate toxicity. In addition, heart- and brain-derived cells showed similar sensitivity, suggesting that the same mode of action might be responsible for the toxicity of glyphosate at the cellular level. The newly developed Pacific white snook brain and heart cell lines could be useful to investigate cellular and molecular mechanisms of toxicity, satisfying the need to reduce the use of animals in experiments. Glyphosate-related toxicological data obtained in the present study will allow us to continue investigating the effects of this herbicide directly on brain and heart fish cells since similar studies have only been carried out on either live organisms or on human cell lines such as neuroblastoma, which are immortalised by oncogenes or similar.

show abstract

Section: Discussionsupporting

confidence: 91%

“…The morphology presented by both cell lines was fibroblastic, with no irregularities becoming evident during this time. Yashwant et al 34 reported similar results when developing gill, liver and skin cell lines from fish.…”

Section: Discussionmentioning

confidence: 71%

The Development and Evaluation of Brain and Heart Cell Lines from a Marine Fish for Use in Xenobiotic-Induced Cytotoxicity Testing

2021

View full text Add to dashboard Cite

show abstract

“…The cox I gene was used as DNA barcodes for the authentication of 67 cell lines [ 20 ]. Similarly, many fish cell lines have been DNA barcoded using cox I [ 21 – 23 ]. Cell line repositories like DSMZ, ATCC use DNA barcoding as a standard method for cell line identification.…”

Section: Cell Line Characterizationmentioning

confidence: 99%

“…SISK and SISS cell lines developed from the kidney and spleen of Lates calcarifer respectively and SIGE cell line developed from the eye of Epinephelus coioides showed their ability to propagate a nodavirus strain [ 32 ]. Yashwanth et al reported the susceptibility of the OCF cell line to NNV [ 23 ]. SSN-1 cell line supported the replication of snakehead fish vesiculovirus (SHVV) which causes great economic loss in fish culture in East Asian countries [ 33 ].…”

Section: Fish Health Managementmentioning

confidence: 99%

Role and relevance of fish cell lines in advanced in vitro research

et al. 2022

Self Cite

View full text Add to dashboard Cite

Introduction Cell line derived from fish has been established as a promising tool for studying many key issues of aquaculture covering fish growth, disease, reproduction, genetics, and biotechnology. In addition, fish cell lines are very useful in vitro models for toxicological, pathological, and immunological studies. The easier maintenance of fish cell lines in flexible temperature regimes and hypoxic conditions make them preferable in vitro tools over mammalian cell lines. Great excitement has been observed in establishing and characterizing new fish cell lines representing diverse fish species and tissue types. The well-characterized and authenticated cell lines are of utmost essential as these represent cellular functions very similar to in vivo state of an organism otherwise it would affect the reproducibility of scientific research. Conclusion The fish cell lines have exhibited encouraging results in several key aspects of in vitro research in aquaculture including virology, nutrition and metabolism, production of vaccines, and transgenic fish production. The review paper reports the cell lines developed from fish, their characterization, and biobanking along with their potential applications and challenges in in vitro research.

show abstract

“…In this study, we deal with partial fins cut from a fish’s body. Cultured fin tissues cut from fish bodies are known to show explants around the tissues in a culture flask 22 , 23 . Therefore, we investigated the cell variations that have experienced the regeneration process, including the differentiation potential if the cells have dedifferentiated.…”

Section: Introductionmentioning

confidence: 99%

Reprocessing seafood waste: challenge to develop aquatic clean meat from fish cells

Tsuruwaka

Shimada

2022

npj Sci Food

View full text Add to dashboard Cite

Fish consumption has been increasing worldwide as per capita consumption of fish rises along with population growth. At the same time, overfishing is increasing all over the world, causing enormous damage to the ecosystem. There is an urgent need to secure sustainable fishery resources to meet the expanding demand for fish. The present study focused on the cells obtained from fish fins, which were often discarded as food waste, and which had the potential to change their morphology with simple treatments, creating the possibility of processing fish fin cells into clean meat (i.e., meat produced in vitro; artificial, lab-cultured meat using tissue engineering techniques). The fin-derived fibroblast-like cells demonstrated an interesting characteristic; changing the sera or culture media supported differentiation of the fibroblast-like cells to various cell morphologies, such as neurofilaments and adipocytes, etc., without genetic manipulation. Furthermore, it was possible to culture the cells in multi-layered and three-dimensional forms that were suitable for processing and shaping. Taking advantage of the cells’ characteristics, ‘aquatic clean meat’ was produced successfully at the prototype stage. Our results suggest that fish fins, which are often treated as waste material, thus, are easy to procure, simple to process, and could be used to create a sustainable food resource.

show abstract

Characterization of a new cell line from ornamental fish Amphiprion ocellaris (Cuvier, 1830) and its susceptibility to nervous necrosis virus

Cited by 27 publications

References 55 publications

The Development and Evaluation of Brain and Heart Cell Lines from a Marine Fish for Use in Xenobiotic-Induced Cytotoxicity Testing

The Development and Evaluation of Brain and Heart Cell Lines from a Marine Fish for Use in Xenobiotic-Induced Cytotoxicity Testing

Role and relevance of fish cell lines in advanced in vitro research

Reprocessing seafood waste: challenge to develop aquatic clean meat from fish cells

Contact Info

Product

Resources

About