Characterization of a new exopolysaccharide produced by Halorubrum sp. TBZ112 and evaluation of its anti-proliferative effect on gastric cancer cells

Hamidi, Masoud; Mirzaei, Rasool; Delattre, Cédric; Khanaki, Korosh; Pierre, Guillaume; Gardarin, Christine; Petit, Elsa; Karimitabar, Fatemeh; Faezi, Sobhan

doi:10.1007/s13205-018-1515-5

Cited by 25 publications

(14 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the final examples, specific rules about the uterine carcinosarcoma involve multiple parameters, including Natronococcus and Terasakiispira . Both microorganisms are functionally correlated with tumorigenesis ( Angiuoli et al, 2008 ; Hamidi et al, 2019 ). Although the lack of direct connections between such two microorganisms with the uterine carcinosarcoma, their upregulation at least confirms the malignant transformation in candidate tissues, validating our prediction.…”

Section: Discussionmentioning

confidence: 99%

Identifying Robust Microbiota Signatures and Interpretable Rules to Distinguish Cancer Subtypes

Chen

Zeng

et al. 2020

Front. Mol. Biosci.

View full text Add to dashboard Cite

Cancer can be generally defined as a cluster of systematic diseases triggered by abnormal cell proliferation and growth. With the development of biological sciences and biotechnologies, the etiology of cancer is partially revealed, including some of the most substantial pathogenic factors [either endogenous (genetics) or exogenous (environmental)]. However, some remaining factors that contribute to the tumorigenesis but have not been analyzed and discussed in detail remain. For instance, some typical correlations between microorganisms and tumorigenesis have been reported already, but previous studies are just sporadic studies on single microorganism-cancer subtype pairs and do not explain and validate the specific contribution of microbiome on tumorigenesis. On the basis of the systematic microbiome analyses of blood and cancer-associated tissues in cancer patients/controls in public domain, we performed interpretable analyses. We identified several core regulatory microorganisms that contribute to the classification of multiple tumor subtypes and established quantitative predictive models for interpretable prediction by using multiple machine learning methods. We also compared the optimal features (microorganisms) and rules identified from microbiome profiles processed using the Kraken and the SHOGUN. Collectively, our study identified new microbiome signatures and their interpretable classification rules for cancer discrimination and carried out reliable methodological comparison for robust cancer microbiome analyses, thereby promoting the development of tumor etiology at the microbiome level.

show abstract

Section: Discussionmentioning

confidence: 99%

Identifying Robust Microbiota Signatures and Interpretable Rules to Distinguish Cancer Subtypes

Chen

Zeng

et al. 2020

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…As the existence of sulfate functional groups and the EPSs bioactivities are directly related, the low cytotoxicity potential of the EPSs from Halorubrum sp. TBZ112 was not unexpected [125].…”

Section: Archaeamentioning

confidence: 95%

Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

2019

View full text Add to dashboard Cite

The organisms thriving under extreme conditions better than any other organism living on Earth, fascinate by their hostile growing parameters, physiological features, and their production of valuable bioactive metabolites. This is the case of microorganisms (bacteria, archaea, and fungi) that grow optimally at high salinities and are able to produce biomolecules of pharmaceutical interest for therapeutic applications. As along as the microbiota is being approached by massive sequencing, novel insights are revealing the environmental conditions on which the compounds are produced in the microbial community without more stress than sharing the same substratum with their peers, the salt. In this review are reported the molecules described and produced by halophilic microorganisms with a spectrum of action in vitro: antimicrobial and anticancer. The action mechanisms of these molecules, the urgent need to introduce alternative lead compounds and the current aspects on the exploitation and its limitations are discussed.

show abstract

“…In consistent with our findings, several studies have shown the non-toxic nature of EPSs on different cell line models. 46,49,50 Regarding the no cytotoxicity effect of the EPSs on normal cell line of MDCK, it seems they have biocompatibility features. The biocompatibility is one of the desirable properties of the natural polymers for use in the formulation of food products, medicines and cosmetics.…”

Section: Antiproliferative Activitymentioning

confidence: 99%

Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

et al. 2020

Self Cite

View full text Add to dashboard Cite

Purpose: Due to the potential industrial and therapeutic applications of the yeast exopolysaccharides (EPSs), there has been an increasing demand to assess these biopolymers with improved characteristics. This study aimed to characterize the EPSs from Rhodosporidium babjevae (ATCC 90942 and IBRC-M 30088) as well as to evaluate their possible antioxidant, emulsifying and antiproliferative activities. Methods: Rhodosporidium babjevae was cultured for 5 days and following isolation of supernatant, EPSs precipitated with adding of cold absolute ethanol and freeze-dried. The EPSs chemical structure was determined by FT-IR, SEM, HPLC-SEC and GC-MS. Additionally the solubility, water holding capacity and emulsifying activity of EPSs were evaluated. In vitro, antioxidant activity was investigated against DPPH, superoxide and hydroxyl radicals. Finally the EPSs consequence on the cell proliferation of human breast adenocarcinoma (MCF-7) and Madin-Darby canine kidney (MDCK) cell lines was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Results: R. babjevae excreted 1.6±0.2 g/L of the EPSs. The EPSs had three fractions with molecular weights of 1.02 ×106 , 5×105 and 2×105 Da. Mannose and glucose were found as the main monosaccharides of the EPSs (84:16 mol%, respectively). The EPSs exhibited emulsifying activity on sun flower oil. The scavenging activities were found to be dose-dependent and higher than hyaluronic acid. Significant difference among the EPSs treatments on the proliferation of MCF-7 and MDCK cell lines was not observed (P>0.05). Conclusion: These results show the interesting potential of the EPSs from R. babjevae as biocompatible compounds for using in food and pharmaceutical fields.

show abstract

Characterization of a new exopolysaccharide produced by Halorubrum sp. TBZ112 and evaluation of its anti-proliferative effect on gastric cancer cells

Cited by 25 publications

References 39 publications

Identifying Robust Microbiota Signatures and Interpretable Rules to Distinguish Cancer Subtypes

Identifying Robust Microbiota Signatures and Interpretable Rules to Distinguish Cancer Subtypes

Halophiles and Their Biomolecules: Recent Advances and Future Applications in Biomedicine

Characterization and Prospective Applications of the Exopolysaccharides Produced by Rhodosporidium babjevae

Contact Info

Product

Resources

About