Characterization of a new gram-negative filamentous bacterium isolated from bulking sludge

Senghas, Elisabeth; Lingens, Franz

doi:10.1007/bf00252373

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…This discrepancy is most likely explained by the non-quantitative nature of PCR-clone libraries which can give skewed representations of the relative abundance of organisms present in a sample largely due to the PCR step (Hugenholtz & Goebel, L. Bjo$ rnsson and others 2001). Chloroflexi-3 is best known from hotspring and hypersaline isolates or clones but does contain isolates such as Herpetosiphon species obtained from full-scale activated sludges (Bradford et al, 1996 ;Senghas & Lingens, 1985 ;Trick & Lingens, 1984) and a clone from a laboratory-scale process (SBR2022, Bond et al, 1995. Herpetosiphon was found to be responsible for bulking in this environment and its role in degradation of macromolecules from influent sewage was speculated upon (Reichenbach, 1992).…”

Section: Discussionmentioning

confidence: 99%

Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removal c cThe EMBL accession numbers for the sequences reported in this paper are X84472 (strain SBR1029 16S rDNA), X84474 (strain SBR1031 16S rDNA), X84498 (strain SBR1064 16S rDNA), X84565 (strain SBR2022 16S rDNA), X84576 (strain SBR2037 16S rDNA) and X84607 (strain SBR2076 16S rDNA).

et al. 2002

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Most filamentous bacteria in biological nutrient removal (BNR) processes have not been identified beyond their morphotype and simple staining reactions. Furthermore, the majority of sludge filaments observed under the microscope do not hybridize to commonly used phylogenetic probes for well characterized bacterial phyla such as the Proteobacteria, Actinobacteria, Firmicutes and BACTEROIDETES: Specific 16S rRNA-targeted oligonucleotide probes were designed for the phylum Chloroflexi (green non-sulfur bacteria) and optimized for use in fluorescence in situ hybridization. Chloroflexi have been implicated in BNR systems by phylogenetic identification of filamentous bacteria isolated by micromanipulation from sludge and culture-independent molecular phylogenetic surveys. The predominant morphotype responding to the probes was filamentous and these filaments were generally abundant in 10 Australian full-scale and two laboratory-scale BNR samples examined. Filamentous bacteria responding to a subdivision 1 Chloroflexi probe were rare in the samples, whereas subdivision 3 Chloroflexi filaments were very common in some sludges. This is in direct contrast to results obtained from molecular phylogenetic surveys of BNR systems where most sludge 16S rDNA clones belong to subdivision 1 and only a few to subdivision 3. It is suggested that filamentous bacteria belonging to the Chloroflexi phylum account for a large fraction of phylogenetically uncharacterized filaments in BNR systems and are likely to be abundant in such systems on a global scale.

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Section: Discussionmentioning

confidence: 99%

Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removal c cThe EMBL accession numbers for the sequences reported in this paper are X84472 (strain SBR1029 16S rDNA), X84474 (strain SBR1031 16S rDNA), X84498 (strain SBR1064 16S rDNA), X84565 (strain SBR2022 16S rDNA), X84576 (strain SBR2037 16S rDNA) and X84607 (strain SBR2076 16S rDNA).

et al. 2002

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“…For example, 16S rRNA gene sequence analyses of many sharing the Eikelboom type 1851 morphotype belong to the Chloroflexi (Beer et al , 2002), and five type 1851 isolates were named Kouleothrix aurantiaca (Kohno et al , 2002). Filamentous members of Herpetosiphon affiliating within Chloroflexi have also been identified in activated sludge (Senghas & Lingens, 1985; Reichenbach, 1992).…”

Section: Ecophysiology Of Filamentous Bacteriamentioning

confidence: 99%

Identity and ecophysiology of filamentous bacteria in activated sludge

et al. 2009

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Excessive growth of filamentous bacteria in activated sludge wastewater treatment plants (WWTPs) can cause serious operational problems. With some filaments there may be the problem of bulking, where inadequate flocculation and settling of the biomass in the secondary clarifier results in a carryover of solids with the final treated liquid effluent. Their proliferation often encourages the development of stable foams on the surface of the reactors, and these foams may impact negatively on plant performance and operation. The availability of culture-independent molecular methods now allows us to identify many of the more common filamentous organisms encountered in WWTPs, which are phylogenetically diverse, affiliating to seven separate bacterial phyla. Furthermore, the extensive data published in the past decade on their in situ behaviour from the application of these culture-independent methods have not been summarized or reviewed critically. Hence, here, we attempt to discuss what we now know about their identity, ecophysiology and ecological niches and its practical value in better managing activated sludge processes. Some of this knowledge is already being applied to control and manage full-scale WWTPs better, and the hope is that this review will contribute towards further developments in this field of environmental microbiology.

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“…The results presented in this paper thus fail to affirm the proposal (Oyaizu et al, 1987) of ChloroJexus being the photosynthetic component of the stromatolites. The 5s RNA of 'Herpetosiphon strain' Senghas Wie 2 (Van den Eynde et al, 1987), originating from exactly the same strain (Senghas & Lingens, 1985) as was used for 16s RNA analysis, was not, contrary to 16s RNA results, recovered in close association with ChloroJlexus and Thermomicrobium 5s RNA. Instead, the 'Herpetosiphon strain' Senghas Wie 2 sequence grouped with a cluster termed 'miscellaneous' in Fig.…”

Section: )mentioning

confidence: 72%

“…were scattered throughout this cluster. Thus, regarding the assignment of strain Senghas Wie 2 to the phylum of green sulphur bacteria and their relatives (Senghas & Lingens, 1985), and the connected suggestion for an assignment of the strain to the genus Herpetosiphon (Gibson et al, 1985;Woese et al, 1985), we urge caution. In any case, in the original paper of Senghas & Lingens (1985), the strain was set well apart from the genus Herpetosiphon on the basis of physiological differences and of differences in fatty acid composition and in DNA G + C content.…”

Section: )mentioning

confidence: 94%

5S rRNA sequences of representatives of the genera Chlorobium, Prosthecochloris, Thermomicrobium, Cytophaga, Flavobacterium, Flexibacter and Saprospira and a discussion of the evolution of eubacteria in general

Eynde

Peer

Perry

et al. 1990

Journal of General Microbiology

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5s rRNA sequences were determined for the green sulphur bacteria Chlorobium limicola, Chlorobium phaeobacteroides and Prosthecochloris aestuarii, for Thermomicrobium roseum, which is a relative of the green non-sulphur bacteria, and for Cytophaga aquatilis, Cytophaga heparina, Cytophaga johnsonae, Flavobacterium breve, Flexibacter sp. and Saprospira grandis, organisms allotted to the phylum 'BacteroidepCytophagaFlavobacterium' and relatives as determined by 16s rRNA analyses. By using a clustering algorithm a dendrogram was constructed from these sequences and from all other known eubacterial5S RNA sequences. The dendrogram showed differences, as well as similarities, with respect to results obtained by 16s RNA analyses. The 5s RNA sequences of green sulphur bacteria were closely related to one another, and to a cluster containing 5s RNA sequences from Bacteroides and its relatives, including Cytophaga uquatilis. 5s RNA sequences of all other representatives of the 'Bacteroides-Cytophaga-Flavobacterium' phylum as distinguished by 1 6 s RNA analysis failed to group with Bacteroides and related clusters. On the basis of 5s RNA sequences, Thermomicrobium roseum clustered with Chlorofixus aurantiacus, as was expected from 16s RNA analysis.

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Characterization of a new gram-negative filamentous bacterium isolated from bulking sludge

Cited by 7 publications

References 29 publications

Identity and ecophysiology of filamentous bacteria in activated sludge

5S rRNA sequences of representatives of the genera Chlorobium, Prosthecochloris, Thermomicrobium, Cytophaga, Flavobacterium, Flexibacter and Saprospira and a discussion of the evolution of eubacteria in general

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