Characterization of a new highly mosquitocidal isolate of Bacillus thuringiensis – An alternative to Bti?

Zhang, Wenfei; Crickmore, N.; George, Zenas Okon; Xie, Li; He, Yanan; Li, Youzhi; Tang, Ji-Liang; Tian, Lingmin; Wang, Xi; Xuanjun, Fang

doi:10.1016/j.jip.2011.11.003

Cited by 20 publications

(16 citation statements)

References 33 publications

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“…We hypothesize that these toxins may be toxic to dipteran insects since they are contained within dipteran subnetworks. Actually, Cry29A was reported to synergize the toxicity of Cry11Bb against Aedes aegypti (Diptera: Culicidae), and Cry50Aa, Cry50Ba, Cry54Ba, Cry68Aa, and Cry70Aa were all found in some strains with high toxicity against mosquitoes ( 52 – 55 ). Similarly, in the coleopteran subnetwork, Cry26 and Cry28 had no reported toxicity; we suspect that they may target coleopteran hosts.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Zheng

Gao

Liu

et al. 2017

mBio

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Understanding the genetic basis of host shifts is a key genomic question for pathogen and parasite biology. The Bacillus cereus group, which encompasses Bacillus thuringiensis and Bacillus anthracis, contains pathogens that can infect insects, nematodes, and vertebrates. Since the target range of the essential virulence factors (Cry toxins) and many isolates is well known, this group presents a powerful system for investigating how pathogens can diversify and adapt to phylogenetically distant hosts. Specialization to exploit insects occurs at the level of the major clade and is associated with substantial changes in the core genome, and host switching between insect orders has occurred repeatedly within subclades. The transfer of plasmids with linked cry genes may account for much of the adaptation to particular insect orders, and network analysis implies that host specialization has produced strong associations between key toxin genes with similar targets. Analysis of the distribution of plasmid minireplicons shows that plasmids with orf156 and orf157, which carry genes encoding toxins against Lepidoptera or Diptera, were contained only by B. thuringiensis in the specialized insect clade (clade 2), indicating that tight genome/plasmid associations have been important in adaptation to invertebrate hosts. Moreover, the accumulation of multiple virulence factors on transposable elements suggests that cotransfer of diverse virulence factors is advantageous in terms of expanding the insecticidal spectrum, overcoming insect resistance, or through gains in pathogenicity via synergistic interactions between toxins.

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Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Zheng

Gao

Liu

et al. 2017

mBio

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“…Insecticidal activity against newly hatched larvae of C. quinquefasciatus was evaluated according to the previously reported methods [17,18]. DMSO was used as the negative control, azadirachtin was used as the positive control, and 10 mL dechlorinated water was used as the blank control.…”

Section: Insecticidal Activities Against Newly Hatched Larvae Of Culementioning

confidence: 99%

Four New Insecticidal Xanthene Derivatives from the Mangrove-Derived Fungus Penicillium sp. JY246

et al. 2019

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Four new xanthene derivatives, penicixanthenes A–D (1–4), and one known compound 5 were isolated from a marine mangrove endophytic fungus Penicillium sp. JY246 that was obtained from the stem of Ceriops tagal. Their structures were determined by detailed NMR, MS spectroscopic data, modified Mosher’s method, and calculated electronic circular dichroism data. All of the isolated compounds were examined for insecticidal activity. Compounds 2 and 3 showed growth inhibition activity against newly hatched larvae of Helicoverpa armigera Hubner with the IC50 values 100 and 200 μg/mL, respectively, and compounds 1, 3, and 4 showed insecticidal activity against newly hatched larvae of Culex quinquefasciatus with LC50 values of 38.5 (±1.16), 11.6 (±0.58), and 20.5 (±1) μg/mL, respectively. The four xanthene derivatives have the potential to be developed as new biopesticides.

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“…The cells were then stained with Coomassie Blue dye solution and observed with bright-field microscopy using a × 100 oil immersion objective [10]. Scanning electron microscopy (EVO MA 10/LS 10, Carl Zeiss Microscopy GmbH, Germany) was used to further clarify the shape of parasporal inclusions [11].…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

“…Strain QHF158 was grown in G-Tris medium as described before [20]. Spore-crystal pellets were harvested and washed three times with ice-cold 0.5 M NaCl containing 1 mM phenylmethylsulfonyl fluoride (PMSF) [5,11]. The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).…”

Section: Spore-crystal Mixture Preparation and Mass Spectrometry Analmentioning

confidence: 99%

Genome of Bacillus sp. strain QHF158 provides insights into its parasporal inclusions encoded by the S-layer gene

et al. 2019

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Bacillus sp. strain QHF158, a Gram-positive, spore-forming and parasporal crystal-secreting bacterium, was isolated from soil of Limushan National Forest Park in China. Here we present the significant feature of parasporal inclusions of this organism, together with the draft genome sequence and annotation. Phylogenetic analysis suggested that strain QHF158 is possibly a novel species, most closely related to Bacillus mycoides. Genome annotation results revealed that strain QHF158 did not contain any typical Cry or Cyt toxin coding gene. Furthermore, the mass spectrometry analyses demonstrated that the parasporal crystalline inclusions were encoded by the orf_05273 gene, with 95% similarity to the S-layer protein (SLP) EA1 of B. mycoides, which indicated that the parasporal crystal from Bacillus sp. strain QHF158 was mainly formed by SLP, instead of the typical Cry or Cyt toxin proteins.

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Characterization of a new highly mosquitocidal isolate of Bacillus thuringiensis – An alternative to Bti?

Cited by 20 publications

References 33 publications

Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Four New Insecticidal Xanthene Derivatives from the Mangrove-Derived Fungus Penicillium sp. JY246

Genome of Bacillus sp. strain QHF158 provides insights into its parasporal inclusions encoded by the S-layer gene

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