Characterization of a new Lactobacillus salivarius strain engineered to express IBV multi-epitope antigens by chromosomal integration

Abstract

To obtain adhesive and safe lactic acid bacteria (LAB) strains for expressing heterologous antigens, we screened LAB inhabitants in intestine of Tibetan chickens by analyzing their adhesion and safety properties and the selected LAB was engineered to express heterologous antigen (UTEpi C-A) based on chromosomal integration strategy. We demonstrated that a new Lactobacillu salivarius TCMM17 strain is strongly adhesive to chicken intestinal epithelial cells, contains no endogenous plasmids, is susceptible to tested antimicrobials, and shows no toxicities. In order to examine the potential of TCMM17 strain as heterogenous antigen delivering vehicle, we introduced a UTEpi C-A expression cassette in its chromosome by constructing a non-replicative plasmid (pORI280-UUTEpi C-AD). The recombinant TCMM17 strain (∆TCMM17) stably was found to keep the gene cassette through 50 generations, and successfully displayed EpiC encoded by the cassette on its surface. This work provides a universal platform for development of novel oral vaccines and expression of further antigens of avian pathogens.

Harnessing the Potential of Lactobacillus Species for Therapeutic Delivery at the lumenal-mucosal Interface

“…Factors such as the Lactobacillus species, antigen, surface display or secretion techniques, adjuvant proteins, and immune cell targeting methods can all be varied to achieve the ideal outcome of protection for any given pathogen. In addition to those listed above, successes against various bacteria such as enteropathogenic E. coli (Figures 5 & 6) [140,150] as well as diarrheal and respiratory coronaviruses and severe acute respiratory syndrome [83,[151][152][153][154][155][156][157][158] have been demonstrated using similar techniques. The development of vaccines for livestock is especially attractive considering the capability to mix cultures into feed rather than employ individual injections to each animal.…”

Study on the transient expression of infectious bronchitis virus spike protein in Nicotiana benthamiana leaves

“…There have been studies on the expression of IBV spike protein in different systems, including bacteria, viral vectors, and transgenic potatoes (Zhou et al, 2004;Cao et al, 2013;Toro et al, 2014). However, none of these studies were made for commercial vaccine production due to some limitations of efficacy or scaling-up (Tatsis et al, 2004;Meeusen et al, 2007;Ma et al, 2016). Plant-derived proteins were shown to have great characteristics to be developed as vaccine candidates such as high safety, low cost and easy scale-up (Gidding et al, 2001;Aswathi et al, 2014).…”