Characterization of a New Lectin of Soybean Vegetative Tissues

Spilatro, Steven R.; Cochran, Graham; Walker, Ruth; Cablish, Khari L.; Bittner, Christine

doi:10.1104/pp.110.3.825

Cited by 29 publications

(17 citation statements)

References 34 publications

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“…These proteins have been the most studied in leguminous species, because they are frequently very abundant in the seeds of these plants and may constitute up to 10% of the total protein [11,12]. Many lectins have been also isolated from other plant tissues and families [13].…”

Section: Introductionmentioning

confidence: 99%

Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds

Carvalho

Silva

Gomes

et al. 2015

International Journal of Biological Macromolecules

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Section: Introductionmentioning

confidence: 99%

Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds

Carvalho

Silva

Gomes

et al. 2015

International Journal of Biological Macromolecules

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“…In legumes lectins play a role in signaling between the plant host and symbiotic nitrogen-fixing bacteria that colonize root nodules (Sharon and Lis, 1989;Kennedy et al, 1995). It has recently been suggested that lectins may play a role in the sequestration of storage proteins into vacuoles in vegetative tissues (Spilatro et al, 1996). Banana MT clones were tentatively classified as either fruit-type or type-2 MTs based on comparisons of their putative translation products with other MT amino acid sequences.…”

Section: Dlscusslonmentioning

confidence: 99%

Differential Gene Expression in Ripening Banana Fruit

1997

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During banana (Musa acuminata L.) fruit ripening ethylene production triggers a developmental cascade that is accompanied by a massive conversion of starch to sugars, an associated burst of respiratory activity, and an increase in protein synthesis. Differential screening of cDNA libraries representing banana pulp at ripening stages 1 and 3 has led to the isolation of 11 nonredundant groups of differentially expressed mRNAs. ldentification of these transcripts by partia1 sequence analysis indicates that two of the mRNAs encode proteins involved in carbohydrate metabolism, whereas others encode proteins thought to be associated with pathogenesis, senescence, or stress responses in plants. Their relative abundance in the pulp and tissue-specific distribution in greenhouse-grown banana plants were determined by northern-blot analyses. The relative abundance of transcripts encoding starch synthase, granule-bound starch synthase, chitinase, lectin, and a type-2 metallothionein decreased in pulp during ripening. Transcripts encoding endochitinase, p-1,3-glucanase, a thaumatin-like protein, ascorbate peroxidase, metallothionein, and a putative senescence-related protein increased early in ripening. The elucidation of the molecular events associated with banana ripening will facilitate a better understanding and control of these processes, and will allow us to attain our long-term goal of producing candidate oral vaccines in transgenic banana plants.

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“…Detailed information about the distribution of a typical legume lectin over the different leaf cells has been reported for the so-called soybean vegetative lectin. This lectin, which is related to but not identical with the soybean seed lectin, occurs in leaves, stems, petioles, and cotyledons of seedlings but not in seeds (Spilatro et al, 1996). Within the leaves, the soybean vegetative lectin was found in bundle sheath and paraveinal mesophyll cells but could not be detected in the palisade mesophyll or spongy mesophyll.…”

Section: Discussionmentioning

confidence: 79%

The Tn Antigen-Specific Lectin from Ground Ivy Is an Insecticidal Protein with an Unusual Physiology

Wang

Hause²,

Peumans³

et al. 2003

Plant Physiology

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Leaves of ground ivy (Glechoma hederacea) contain a lectin (called Gleheda) that is structurally and evolutionary related to the classical legume lectins. Screening of a population of wild plants revealed that Gleheda accounts for more than one-third of the total leaf protein in some clones, whereas it cannot be detected in other clones growing in the same environment. Gleheda is predominantly expressed in the leaves where it accumulates during early leaf maturation. The lectin is not uniformly distributed over the leaves but exhibits a unique localization pattern characterized by an almost exclusive confinement to a single layer of palisade parenchyma cells. Insect feeding trials demonstrated that Gleheda is a potent insecticidal protein for larvae of the Colorado potato beetle (Leptinotarsa decemlineata). Because Gleheda is not cytotoxic, it is suggested that the insecticidal activity is linked to the carbohydrate-binding specificity of the lectin, which as could be demonstrated by agglutination assays with different types of polyagglutinable human erythrocytes is specifically directed against the Tn antigen structure (N-acetylgalactosamine O-linked to serine or threonine residues of proteins).

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Characterization of a New Lectin of Soybean Vegetative Tissues

Cited by 29 publications

References 34 publications

Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds

Purification, characterization and antibacterial potential of a lectin isolated from Apuleia leiocarpa seeds

Differential Gene Expression in Ripening Banana Fruit

The Tn Antigen-Specific Lectin from Ground Ivy Is an Insecticidal Protein with an Unusual Physiology

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