Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor

Forment, Javier; Tzfadia, Oren; Forment, Javier; Presa, Silvia; Rogachev, Ilana; Meir, Sagit; Orzáez, Diego; Aharoni, Aspah; Granell, Antonio

doi:10.1104/pp.16.00282

Cited by 54 publications

(42 citation statements)

References 65 publications

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“…No substantial differences in carotenoids were found between y and SM, except the higher all-trans-neoxanthin levels in y_gf and y_r. Conversely, tocochromanol metabolism was altered in y mutant berries as there was a decrease in tocopherols, mainly affecting y and y_r, confirming previous reports [15,59].…”

Section: Biochemical Changes In Fruits Of Colorless Fruit Epidermis Asupporting

confidence: 89%

Color Mutations Alter the Biochemical Composition in the San Marzano Tomato Fruit

et al. 2020

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San Marzano (SM) is a traditional Italian landrace characterized by red elongated fruits, originating in the province of Naples (Italy) and cultivated worldwide. Three mutations, yellow flesh (r), green flesh (gf) and colorless fruit epidermis (y) were introduced into SM by backcross and the resulting introgression lines (ILs) produced the expected yellow, brown and pink fruit variants. In addition, ILs carrying double combinations of those mutations were obtained. The six ILs plus the SM reference were analyzed for volatile (VOC), non-polar (NP) and polar (P) metabolites. Sixty-eight VOCs were identified, and several differences evidenced in the ILs; overall gf showed epistasis over r and y and r over y. Analysis of the NP component identified 54 metabolites; variation in early carotenoids (up to lycopene) and chlorophylls characterized respectively the ILs containing r and gf. In addition, compounds belonging to the quinone and xanthophyll classes were present in genotypes carrying the r mutation at levels higher than SM. Finally, the analysis of 129 P metabolites evidenced different levels of vitamins, amino acids, lipids and phenylpropanoids in the ILs. A correlation network approach was used to investigate metabolite–metabolite relationships in the mutant lines. Altogether these differences potentially modified the hedonistic and nutritional value of the berry. In summary, single and combined mutations in gf, r and y generated interesting visual and compositional diversity in the SM landrace, while maintaining its original typology.

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Section: Biochemical Changes In Fruits Of Colorless Fruit Epidermis Asupporting

confidence: 89%

Color Mutations Alter the Biochemical Composition in the San Marzano Tomato Fruit

et al. 2020

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“…By means of qPCRs, we confirmed the expression patterns of CHS1 and CHS2 ( Figure S2A,B ). We additionally found that several genes encoding positive regulators of flavonoid (MYB12; [ 91 , 92 , 93 , 94 ]) or anthocyanin biosynthesis (AN2/MYB75, JAF13/bHLH90; [ 95 ]) were also down-regulated in Tu+Al. The anthocyanin biosynthesis regulatory gene ANT1/MYB113 [ 96 ] was not differentially regulated in response to mite feeding.…”

Section: Resultsmentioning

confidence: 99%

Distinct Signatures of Host Defense Suppression by Plant-Feeding Mites

Schimmel

Alba

Wybouw

et al. 2018

IJMS

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Tomato plants are attacked by diverse herbivorous arthropods, including by cell-content-feeding mites, such as the extreme generalist Tetranychus urticae and specialists like Tetranychus evansi and Aculops lycopersici. Mite feeding induces plant defense responses that reduce mite performance. However, T. evansi and A. lycopersici suppress plant defenses via poorly understood mechanisms and, consequently, maintain a high performance on tomato. On a shared host, T. urticae can be facilitated by either of the specialist mites, likely due to the suppression of plant defenses. To better understand defense suppression and indirect plant-mediated interactions between herbivorous mites, we used gene-expression microarrays to analyze the transcriptomic changes in tomato after attack by either a single mite species (T. urticae, T. evansi, A. lycopersici) or two species simultaneously (T. urticae plus T. evansi or T. urticae plus A. lycopersici). Additionally, we assessed mite-induced changes in defense-associated phytohormones using LC-MS/MS. Compared to non-infested controls, jasmonates (JAs) and salicylate (SA) accumulated to higher amounts upon all mite-infestation treatments, but the response was attenuated after single infestations with defense-suppressors. Strikingly, whereas 8 to 10% of tomato genes were differentially expressed upon single infestations with T. urticae or A. lycopersici, respectively, only 0.1% was altered in T. evansi-infested plants. Transcriptome analysis of dual-infested leaves revealed that A. lycopersici primarily suppressed T. urticae-induced JA defenses, while T. evansi dampened T. urticae-triggered host responses on a transcriptome-wide scale. The latter suggests that T. evansi not solely down-regulates plant gene expression, but rather directs it back towards housekeeping levels. Our results provide valuable new insights into the mechanisms underlying host defense suppression and the plant-mediated facilitation of competing herbivores.

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“…Identification of carotenoids was performed as reported previously (Liu et al, 2014). Phenylpropanoid analysis was performed by comparing chromatographic and spectral properties with standards and reference DAD-HRMS spectra as previously reported (Fernandez-Moreno et al, 2016;Iijima et al, 2008;Moco et al, 2006). For each analysis, at least three biological replicates were performed.…”

Section: Nonpolar and Semi-polar Metabolite Analysesmentioning

confidence: 99%

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

Diretto

Frusciante

Fabbri

et al. 2019

Plant Biotechnology Journal

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Summary Tomato fruit ripening is controlled by the hormone ethylene and by a group of transcription factors, acting upstream of ethylene. During ripening, the linear carotene lycopene accumulates at the expense of cyclic carotenoids. Fruit‐specific overexpression of LYCOPENE β‐CYCLASE (LCYb) resulted in increased β‐carotene (provitamin A) content. Unexpectedly, LCYb‐overexpressing fruits also exhibited a diverse array of ripening phenotypes, including delayed softening and extended shelf life. These phenotypes were accompanied, at the biochemical level, by an increase in abscisic acid (ABA) content, decreased ethylene production, increased density of cell wall material containing linear pectins with a low degree of methylation, and a thicker cuticle with a higher content of cutin monomers and triterpenoids. The levels of several primary metabolites and phenylpropanoid compounds were also altered in the transgenic fruits, which could be attributed to delayed fruit ripening and/or to ABA. Network correlation analysis and pharmacological experiments with the ABA biosynthesis inhibitor, abamine, indicated that altered ABA levels were a direct effect of the increased β‐carotene content and were in turn responsible for the extended shelf life phenotype. Thus, manipulation of β‐carotene levels results in an improvement not only of the nutritional value of tomato fruits, but also of their shelf life.

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Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor

Cited by 54 publications

References 65 publications

Color Mutations Alter the Biochemical Composition in the San Marzano Tomato Fruit

Color Mutations Alter the Biochemical Composition in the San Marzano Tomato Fruit

Distinct Signatures of Host Defense Suppression by Plant-Feeding Mites

Manipulation of β‐carotene levels in tomato fruits results in increased ABA content and extended shelf life

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