Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil

Maciel, Scheila da Conceição; Silva, Ricardo Ferreira da; Reis, M. S.; Jadão, Adriana Salomão; Rosa, Daniel Dias; Giampan, José Segundo; Kitajima, Elliot Watanabe; Rezende, J. A. M.; Camargo, Luís Eduardo Aranha

doi:10.1590/s0103-90162011000600013

Cited by 23 publications

(5 citation statements)

References 8 publications

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“…This two-component "timebomb" would be more effective against chewing insects compared to piercing insects, as the latter is less likely to be exposed to both precursors (or enzymes and substrates) simultaneously. Documented natural pests of C. roseus are, indeed, limited to arthropods with piercing-sucking mouthparts, including aphids (Maciel et al, 2011;Samad et al, 2008), whiteflies (Francis et al, 2016), scale insects (Kondo, Amalia Ramos-Portilla, Peronti, & Gullan, 2016), the pink hibiscus mealybug (Chong, Aristizábal, & Arthurs, 2015), and two-spotted spider mite (Haque, Islam, Naher, & Haque, 2011).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Liu,

Shi,

Patra

et al. 2024

Preprint

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The evolutionary arms-race between plants and insects has led to key adaptive innovations that drive diversification. Alkaloids are well-documented anti-herbivory compounds in plant chemical defenses, but how these specialized metabolites are allocated to cope with both biotic and abiotic stresses concomitantly is largely unknown. To examine how plants prioritize their metabolic resources responding to herbivory and cold, we integrated dietary toxicity bioassay in insects with co-expression analysis, hierarchical clustering, promoter assay, and protein-protein interaction in plants.Catharanthus roseus,a medicinal plant known for its insecticidal property against chewing herbivores, produces two terpenoid indole alkaloid monomers, vindoline and catharanthine. Individually, they exhibited negligible toxicity againstManduca sexta,a chewing herbivore; their condensed product, anhydrovinblastine, however, was highly toxic. Such unique insecticidal mode of action demonstrates that terpenoid indole alkaloid “timebomb” can only be activated when the two spatially isolated monomeric precursors are dimerized by herbivory. Without initial selection pressure and apparent fitness costs, this adaptive chemical defense against herbivory is innovative and sustainable.The biosynthesis of insecticidal terpenoid indole alkaloids is induced by herbivory but suppressed by cold. Here, we identified a transcription factor,Herbivore InducedVindoline-geneExpression (HIVE), that coordinates the production of terpenoid indole alkaloids in response to herbivory and cold stress. The HIVE-mediated transcriptional reprogramming allows this herbaceous perennial to allocate their metabolic resources for chemical defense at a normal temperature, when herbivory pressure is high, but switches to cold tolerance under a cooler temperature, when insect infestation is secondary.

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Section: Discussionmentioning

confidence: 99%

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Liu,

Shi,

Patra

et al. 2024

Preprint

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“…(1998). The obtained RNA was used for virus detection by one‐step reverse transcription‐polymerase chain reaction (RT‐PCR) with the potyvirus universal primers WCIEN/PV1, which amplifies a fragment of 800 bp comprising part of the coat protein (CP) gene and the 3′ non‐translated region (Maciel et al., 2011). The obtained amplicons were sent for nucleotide sequencing, in the sense direction, at the Institute of Biotechnology (IBTEC) of the University of the State of São Paulo, Brazil.…”

Section: Figurementioning

confidence: 99%

Biological and molecular characterization of bidens mosaic virus infecting patchouli (Pogostemon cablin) in Brazil

Martines,

Favara,

Marubayashi

et al. 2023

Journal of Phytopathology

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Six patchouli (Pogostemon cablin Benth.) plants showing symptoms of viral infection were found in Brazil. Transmission electron microscopy revealed flexuous particles and characteristic pinwheel‐shaped cytoplasmic inclusions of potyvirus in sap and cells of symptomatic leaves. Potyvirus infection was confirmed through RT‐PCR, using universal primers followed by nucleotide sequencing. The obtained sequences, comprising part of the CP gene and the 3′ non‐translated region, showed 97.71%–97.87% identity with an isolate of bidens mosaic virus (BiMV). The nucleotide sequences of the CP, HC‐Pro and CI genes of BiMV isolates from patchouli plants were obtained through RT‐PCR with specific primers followed by nucleotide sequencing. The obtained sequences showed 95.78%–97.1% identities with BiMV isolates from Brazil. One BiMV isolate from a patchouli infected plant was sap‐transmitted to different plant species. Chenopodium amaranticolor exhibited local lesions, Bidens pilosa developed leaf mosaic, and Nicotiana benthamiana exhibited chlorosis and stunting. The BiMV isolate was transmitted from infected patchouli plant to B. pilosa using Myzus persicae aphids. This is the first confirmation of patchouli infected with BiMV in Brazil. Further studies are necessary to assess the incidence of this potyvirus in commercial patchouli crops, as well as the damage caused to plant development and the quantity and quality of essential oils produced.

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“…Total RNA was extracted from eight symptomatic C. chinense plants using a PureLink Viral RNA/DNA kit (Thermo Fisher Scientific, Waltham, USA) following the manufacturer's recommendations. One-step RT-PCR was performed using universal primers PV1/SP6 and WCIEN-sense, for detection of potyviruses (Mackenzie et al 1998;Maciel et al 2011), and specific primers for the coat protein gene of cucumber mosaic virus (CMV) (Wylie et al 1993), which amplify fragments of 800 and 480 bp, respectively. Amplicons of the expected size for each virus were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, South Korea).…”

Section: Amazonicamentioning

confidence: 99%

Ocurrence of pepper yellow mosaic virus and cucumber mosaic virus on Capsicum chinense in the state of Amazonas, Brazil

et al. 2020

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The habanero chilli pepper, Capsicum chinense is an important crop in the Amazon Basin, mainly grown by small-scale producers. Capsicum chinense plants in an experimental field in the northern Brazilian state of Amazonas were found exhibiting characteristic symptoms of viral infection. Leaf sap from symptomatic plants examined under a transmission electron microscope revealed the presence of elongated flexuous particles and isometric particles. Using molecular assays, the viruses were identified as pepper yellow mosaic virus (PepYMV) and cucumber mosaic virus (CMV). Aphids, identified as Aphis gossypii, were found colonizing the C. chinense plants in the field and may be the vector for both PepYMV and CMV. We report the first occurrence of these viruses infecting C. chinense in the state of Amazonas. Ocorrência do pepper yellow mosaic virus e cucumber mosaic virus emCapsicum chinense no estado do Amazonas, Brasil

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Characterization of a new potyvirus causing mosaic and flower variegation in Catharanthus roseus in Brazil

Cited by 23 publications

References 8 publications

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Transcriptional reprogramming deploys a compartmentalized “timebomb” inCatharanthus roseusto fend off chewing herbivores

Biological and molecular characterization of bidens mosaic virus infecting patchouli (Pogostemon cablin) in Brazil

Ocurrence of pepper yellow mosaic virus and cucumber mosaic virus on Capsicum chinense in the state of Amazonas, Brazil

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