Characterization of a NK-lysin antimicrobial peptide gene from channel catfish

Wang, Qun; Bao, Baolong; Wang, Yaping; Peatman, Eric; Liu, Zhanjiang

doi:10.1016/j.fsi.2005.05.005

Cited by 71 publications

(36 citation statements)

References 43 publications

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“…Much has been learned in recent years concerning genes involved in both the innate and adaptive immunity (e.g., Wilson et al, 1997;Miller et al, 1998;Antao et al, 2001;Hawke et al, 2001;Zou et al, 2003). Recently, we have characterized a set of 27 CC chemokines Peatman et al, 2005), 7 CXC chemokines Chen et al, 2005), and 4 antimicrobial peptides (Bao et al, 2005, in press;Xu et al, 2005;Wang et al, 2006). In this work, we further characterized genomic structure and organization of three NK-lysin genes.…”

Section: Discussionmentioning

confidence: 97%

“…We previously reported a NK-lysin cDNA (Wang et al, 2006). The cDNA sequences were used to design overgo probes (Bao et al, 2005, in press;Xu et al, 2005) for hybridization to BAC filters.…”

Section: Assigning Nk-lysin Gene To Bac Clonesmentioning

confidence: 99%

“…A search of dbEST using the previously identified NK-lysin cDNA (Wang et al, 2006) identified 166 catfish ESTs with significant sequence similarities (<10 −8 ) to the query sequence. These sequences were downloaded from the GenBank in Vector NTI Contig Express and subjected to cluster analysis.…”

Section: Identification Of Novel Nk-lysin Transcriptsmentioning

confidence: 99%

“…The cDNA sequence of the type 1 NK-lysin was previously reported (Wang et al, 2006, Accession number AY934592), while the type 2 and type 3 NK-lysin are novel. The entire cDNAs of type 2 and type 3 NK-lysin were sequenced and their sequences have been deposited to GenBank with Accession numbers of DQ153186 and DQ153187, respectively.…”

Section: Identification Of Novel Nk-lysin Transcriptsmentioning

confidence: 99%

“…Their primary sequences are rich in positively charged amino acids and the sulfite bond-forming cysteines. We previously reported a NK-lysin cDNA from channel catfish (Wang et al, 2006). BLAST searches of the draft genome sequences of the closely related zebrafish species reveal multiple copies of the NK-lysin gene.…”

Section: Introductionmentioning

confidence: 99%

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NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis

Wang

et al. 2006

Molecular Immunology

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Section: Discussionmentioning

confidence: 97%

Section: Assigning Nk-lysin Gene To Bac Clonesmentioning

confidence: 99%

Section: Identification Of Novel Nk-lysin Transcriptsmentioning

confidence: 99%

Section: Identification Of Novel Nk-lysin Transcriptsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis

Wang

et al. 2006

Molecular Immunology

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Expression profiles of four Nile Tilapia innate immune genes during early stages of Aeromonas veronii infection

Bakry,

Emeish,

Embark

et al. 2024

J Aqua Anim Hlth

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ObjectiveDuring Egypt's hot summer season, Aeromonas veronii infection causes catastrophic mortality on Nile Tilapia Oreochromis niloticus farms. Egypt is ranked first in aquaculture production in Africa, sixth in aquaculture production worldwide, and third in global tilapia production. This study aimed to investigate, at the molecular level, the early innate immune responses of Nile Tilapia to experimental A. veronii infection.MethodsThe relative gene expression, co‐expression clustering, and correlation of four selected immune genes were studied by quantitative real‐time polymerase chain reaction in four organs (spleen, liver, gills, and intestine) for up to 72 h after a waterborne A. veronii challenge. The four genes studied were nucleotide‐binding oligomerization domain 1 (NOD1), lipopolysaccharide‐binding protein (LBP), natural killer‐lysin (NKL), and interleukin‐1 beta (IL‐1β).ResultThe four genes showed significant transcriptional upregulation in response to infection. At 72 h postchallenge, the highest NOD1 and IL‐1β expression levels were recorded in the spleen, whereas the highest LBP and NKL expression levels were found in the gills. Pairwise distances of the data points and the hierarchical relationship showed that NOD1 clustered with IL‐1β, whereas LBP clustered with NKL; both genes within each cluster showed a significant positive expression correlation. Tissue clustering indicated that the responses of only the gill and intestine exhibited a significant positive correlation.ConclusionThe results suggest that NOD1, LBP, NKL, and IL‐1β genes play pivotal roles in the early innate immune response of Nile Tilapia to A. veronii infection, and the postinfection expression profile trends of these genes imply tissue‐/organ‐specific responses and synchronized co‐regulation.

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A review on comparative mechanistic studies of antimicrobial peptides against archaea

Varnava

Ronimus

Sarojini

2017

Biotech & Bioengineering

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Archaea was until recently considered as a third domain of life in addition to bacteria and eukarya but recent studies support the existence of only two superphyla (bacteria and archaea). The fundamental differences between archaeal, bacterial, and eukaryal cells are probably the main reasons for the comparatively lower susceptibility of archaeal strains to current antimicrobial agents. The possible emerging pathogenicity of archaea and the role of archaeal methanogens in methane emissions, a potent greenhouse gas, has led many researchers to examine the sensitivity patterns of archaea and make attempts to find agents that have significant anti-archaeal activity. Even though antimicrobial peptides (AMPs) are well known with several published reviews concerning their mode of action against bacteria and eukarya, to our knowledge, to date no reviews are available that focus on the action of these peptides against archaea. Herein, we present a review on all the peptides that have been tested against archaea. In addition, in an attempt to shed more light on possible future work that needs to be performed we have included a brief overview of the chemical characteristics, spectrum of activity, and the known mechanism of action of each of these peptides against bacteria and/or fungi. We also discuss the nature of and key physiological differences between Archaea, Bacteria, and Eukarya that are relevant to the development of anti-archaeal peptides. Despite our relatively limited knowledge about archaea, available data suggest that AMPs have an even broader spectrum of activity than currently recognized.

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Characterization of a NK-lysin antimicrobial peptide gene from channel catfish

Cited by 71 publications

References 43 publications

NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis

NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis

Expression profiles of four Nile Tilapia innate immune genes during early stages of Aeromonas veronii infection

A review on comparative mechanistic studies of antimicrobial peptides against archaea

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