Characterization of a novel alternavirus infecting the fungal pathogen Fusarium solani

Lutz, Tobias; Japić, Elma; Bien, Steffen; Langer, Gitta Jutta; Heinze, Cornelia

doi:10.1016/j.virusres.2022.198817

Cited by 18 publications

(21 citation statements)

References 38 publications

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“…1 ). Like other members of the proposed family " Alternaviridae " [ 10 , 12 ], the 5’ UTRs are capped (Supplementary Fig. 1) and their 3’ UTRs are polyadenylated.…”

Section: Sequence Propertiesmentioning

confidence: 99%

“…Only 26.67% aa sequence identity was shared with the hypothetical protein P2 of AaV1 (E-value: 2e-16; YP_001976150.1). Structural analysis of AaV1 by Wu et al [ 12 ] and of FsAV1 by Lutz et al [ 10 ] revealed that P2 is not part of the virus particle, and it is therefore hypothesized to be a non-structural protein, but its function is unknown.…”

Section: Sequence Propertiesmentioning

confidence: 99%

“…Members of this proposed family possess a genome consisting of three to four monocistronic dsRNA segments, which range from 1.4 kbp (dsRNA 4) to 3.6 kbp (dsRNA 1) [ 3 – 11 ], and a polyA tail at the 3’ end. Wu et al [ 12 ] and Lutz et al [ 10 ] experimentally showed that the 5’ ends of the AaV1 and of Fusarium solani alternavirus 1 (FsAV1) genome segments are capped. It is not known if other members of this family are also capped at their 5’ end.…”

mentioning

confidence: 99%

“…It is not known if other members of this family are also capped at their 5’ end. It was also shown that the proteins encoded on segment 1 and on segment 3 are structural and were proposed to represent the RdRPs and the capsid proteins, respectively [ 4 , 10 , 12 ]. The proteins encoded on dsRNA 2 and dsRNA 4 are suggested to be non-structural and are of unknown function.…”

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confidence: 99%

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Complete genome sequence of a novel alternavirus infecting the fungus Ilyonectria crassa

2023

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A novel dsRNA mycovirus named Ilyonectria crassa alternavirus 1 (IcAV1) was found in Ilyonectria crassa isolate NW-FVA 1829. The fungus was isolated from an ash (Fraxinus excelsior L.) necrotic trunk disc infected with Hymenoscyphus fraxineus [(T. Kowalski) Baral, Queloz, Hosoya] causing ash dieback. The complete genome of IcAV1 is composed of three segments, each containing a single ORF on the positive-sense RNA. The extreme 5’ UTRs of dsRNA 1 (3604 bp), dsRNA 2 (2547 bp), and dsRNA 3 (2518 bp) share a conserved hexadecamer sequence (5’-GGCTGTGTGTTTAGTT-3’) and are capped. The 3’ UTRs are polyadenylated. In silico analysis showed that the viral RdRP is encoded on dsRNA 1 and the capsid-protein subunits are encoded on dsRNA 3. Maximum-likelihood analysis of the aa sequence of the viral RdRP showed that IcAV1 clusters with alternaviruses from Fusarium spp., while the type member of the proposed family "Alternaviridae", Alternaria alternata virus 1 (AaV1), formed a clade together with Stemphylium lycopersici mycovirus (SlV). The function of the protein encoded on segment 2 is unknown. Based on its genome organization and its phylogenetic position, IcAV1 is suggested to be a new member of the proposed family "Alternaviridae". This is the first report of a mycovirus infecting I. crassa.

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“…1 ). Like other members of the proposed family " Alternaviridae " [ 10 , 12 ], the 5’ UTRs are capped (Supplementary Fig. 1) and their 3’ UTRs are polyadenylated.…”

Section: Sequence Propertiesmentioning

confidence: 99%

Section: Sequence Propertiesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Complete genome sequence of a novel alternavirus infecting the fungus Ilyonectria crassa

2023

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“…To date, eleven mycoviruses have been designated as alternaviruses in the proposed family Alternaviridae. These include Aspergillus mycovirus 341 (AsV341) [ 19 ], Alternaria alternata virus 1 (AaV1) [ 20 , 21 ], Aspergillus foetidus virus (AfV) [ 22 ], Fusarium poae alternavirus 1 (FpAV1) [ 23 ], Fusarium graminerarum alternairus 1 (FgAV1) [ 24 ], Aspergillus heteromorphus alternavirus 1 (AheAV1) [ 25 ], Fusarium incarnatum alternavirus 1 (FiAV1) [ 7 ], Fusarium oxysporum alternavirus 1 (FoAV1) [ 26 ], Fusarium solani alternavirus 1 (FsAV1) [ 27 ], Cordyceps chanhua alternavirus 1 (CcAV1) [ 28 ], and Stemphylium lycopersici alternavirus 1 (SlAV1) [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Zhang

Hua

et al. 2023

Viruses

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A novel virus with a double-stranded RNA (dsRNA) genome was isolated from Fusarium avenaceum strain GS-WW-224, the causal agent of potato dry rot. The virus has been designated as Fusarium avenaceum alternavirus 1 (FaAV1). Its genome consists of two dsRNA segments, 3538 bp (dsRNA1) and 2477 bp (dsRNA2) in length, encoding RNA-dependent RNA polymerase (RdRp) and a hypothetical protein (HP), respectively. The virions of FaAV1 are isometric spherical and approximately 30 nm in diameter. Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of RdRp and HP indicated that FaAV1 appears to be a new member of the proposed family Alternaviridae. No significant differences in colony morphology and spore production were observed between strains GS-WW-224 and GS-WW-224-VF, the latter strain being one in which FaAV1 was eliminated from strain GS-WW-224. Notably, however, the dry weight of mycelial biomass of GS-WW-224 was higher than that of mycelial biomass of GS-WW-224-VF. The depth and the width of lesions on potato tubers caused by GS-WW-224 were significantly greater, relative to GS-WW-224-VF, suggesting that FaAV1 confers hypervirulence to its host, F. avenaceum. Moreover, FaAV1 was successfully transmitted horizontally from GS-WW-224 to ten other species of Fusarium, and purified virions of FaAV1 were capable of transfecting wounded hyphae of the ten species of Fusarium. This is the first report of an alternavirus infecting F. avenaceum and conferring hypervirulence.

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Molecular characterization of two new alternaviruses identified in members of the fungal family Nectriaceae

et al. 2023

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Since the first report in 2009, at least ten additional viruses have been identified and assigned to the proposed virus family Alternaviridae. Here we report two new mycoviruses tentatively assigned to this family, both identified as members of the fungal family Nectriaceae, which were isolated from surface-disinfected apple roots (Malus x domestica, Borkh.) affected by apple replant disease (ARD). ARD is a highly complex, worldwide-occurring disease resulting from plant reactions to a disturbed (micro)-biome and leads to high economic losses every year. The first alternavirus characterized in this study was identified in a Dactylonectria torresensis isolate. The virus was tentatively named dactylonectria torresensis alternavirus 1 (DtAV1) as the first member of the proposed new species Alternavirus dactylonectriae. The second virus was identified in an isolate of Ilyonectria robusta and was tentatively named ilyonectria robusta alternavirus 1 (IrAV1) as the first member of the proposed new species Alternavirus ilyonectriae. Full genomic sequences of the viruses were determined and are presented. Further, we found hints for putative components of a methyl transferase machinery using in silico approaches. This putative protein domain is encoded by segment 2. However, this result only establishes the basis for subsequent studies in which the function must be confirmed experimentally in vitro. Thus, this is the first study where a function is predicted to all three genomic segments within the group of the alternaviruses. These findings provide further insights into the virome of ARD-associated fungi and are therefore another brick in the wall of understanding the complexity of the disease.

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Characterization of a novel alternavirus infecting the fungal pathogen Fusarium solani

Cited by 18 publications

References 38 publications

Complete genome sequence of a novel alternavirus infecting the fungus Ilyonectria crassa

Complete genome sequence of a novel alternavirus infecting the fungus Ilyonectria crassa

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Molecular characterization of two new alternaviruses identified in members of the fungal family Nectriaceae

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