Characterization of a novel anthocyanin profile in wild black raspberry mutants: An opportunity for studying the genetic control of pigment and color

Abstract

Bramble Caneberry Cyanidin-3-rutinoside Rhamnose Rhamnosyltransferase A B S T R A C TThe type and amount of anthocyanins in raspberries, and other small fruits, has recently received increased attention. Black raspberry (Rubus occidentalis L.), in particular, has long been recognized as a rich source of anthocyanins and has been the focus of many recent studies examining their potential health benefits. In this study, we characterized a novel anthocyanin profile found in seedlings of two wild black raspberry populations collected from South Dakota, USA. Seedlings from these populations lack pigments glycosylated with rutinoside in their fruit, have elevated levels of cyanidin-3-sambubioside, and contain a small but significant amount of pelargonidin-3-glucoside, a pigment reported only once previously in black raspberry. Affected fruit also have lower than typical total anthocyanins (77.5-134.4 mg 100 mL À1 ). Based on the available evidence, we believe the plants have a mutation in the gene encoding anthocyanidin-3-glycoside rhamnosyltransferase (3RT), providing a unique opportunity to identify and study one of the major genes in the anthocyanin pathway and its effect on fruit anthocyanins and color.Published by Elsevier Ltd.

Anthocyanin analyses of Vaccinium fruit dietary supplements

“…; Dossett et al. , ). A representative anthocyanin profile of rabbiteye ( V. ashei Reade) blueberries can be found in Lohachoompol et al.…”

“…3B). Using anthocyanin profiles to distinguish different species has been previously demonstrated in other fruit (Lee 2013(Lee , 2014(Lee , 2015Gardana et al 2014), and is well established in aiding in chemotaxonomy (Hong and Wrolstad 1990a,b;Vorsa et al 2003;Penman et al 2006;Finn 2007, 2012;Latti et al 2008;Lohachoompol et al 2008;Debnath and Sion 2009;Vasco et al 2009;Gardana et al 2014;Finn et al 2014;Dossett et al 2008Dossett et al , 2011. A representative anthocyanin profile of Figure 4.…”

Establishing a case for improved food phenolic analysis

“…Continued refinements of sample handling, preparation, hydrolysis, and purification steps that optimize phenolic retention will advance research evaluations and production processes. Identity of the major black raspberry ( Rubus occidentalis L.) anthocyanin was clarified (Dossett et al. , , ; Lee et al. ).…”

“…This emphasizes the importance of correct identification of the starting material for the benefit of other scientific studies downstream. A wild black raspberry genotype bearing fruit with a unique anthocyanin profile was discovered (Dossett et al. ). The plant was part of a wild‐germplasm collection that was grown in a research plot as part of investigations on genetic diversity for breeding improved black raspberry cultivars.…”

Physico-chemical and bioactive compounds characterization of apple ‘Gala’ mutants harvested at two different time points

“…Among the phytochemicals phenolic compounds have received much attention because of their antioxidant activity (Tsuda et al 1994, Leyva-Corral et al 2016). Many studies show that apples are a rich source of phenolic compounds, whose activity and concentration vary with the cultivar, maturity stage, environmental conditions, biotic and abiotic stress and the part of the fruit (Kjersti et al 2004, Van Der Sluice et al 2001, Dossett et al 2011, Lee 2012.…”