Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition

Meng, Zhenxiang; Ma, Jing; Sun, Zhanying; Yang, Chengjian; Leng, Jing; Zhu, Weiyun; Cheng, Yanfen

doi:10.1016/j.aninu.2023.01.004

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Cited by 3 publications

References 65 publications

(118 reference statements)

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Microbiome engineering and ruminants

Zhang,

Jin,

Liu

et al. 2025

Human and Animal Microbiome Engineering

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Microbiome engineering and ruminants

Zhang,

Jin,

Liu

et al. 2025

Human and Animal Microbiome Engineering

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Exploring biomimetic potential of ruminant digestion strategies for lignocellulosic biomass utilization: A comprehensive review

Han,

Chang,

Xiang

et al. 2023

Renewable and Sustainable Energy Reviews

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Characterization of Novel Multifunctional Xylanase from Rumen Metagenome and Its Effects on In Vitro Microbial Fermentation of Wheat Straw

Zhang,

Qiu,

Zhao

et al. 2024

Fermentation

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This study investigated the characterization of a novel multifunctional enzyme, RuXyn394, derived from the metagenome of beef cattle rumen, and its impact on the in vitro microbial fermentation of wheat straw. RuXyn394, a member of the glycosyl hydrolase 11 family, displayed optimal activity under diverse pH and temperature conditions: xylanase at pH 5.5 and 50 °C, acetyl esterase at pH 6.5 and 60 °C, exoglucanase at pH 7.0 and 50 °C, and endoglucanase at pH 6.0 and 50 °C. The enzyme’s xylanase, endoglucanase, and exoglucanase activities exhibited remarkable pH stability across the range of pH 3–8 and maintained a relatively stable performance at temperatures from 20 to 50 °C, 20 to 60 °C, and 20 to 70 °C, respectively. The xylanase function, with the highest kcat/Km ratio, was identified as the predominant activity of RuXyn394. The enzyme’s various functions responded uniquely to metal ions; notably, the addition of 5 mM K+ significantly boosted the activities of xylanase, exoglucanase, and endoglucanase by 55.5%, 53.5%, and 16.4%, respectively, without affecting its acetyl esterase activity. Over the course of three time points (30 min, 60 min, 120 min), the degradation products of wheat straw xylan, including xylopentaose, xylotetraose, xylotriose, xylobiose, xylose, and total xylooligosaccharides, constituted an average of 18.4%, 33.7%, 20.6%, 22.9%, 4.3%, and 95.7% of the total products, respectively. RuXyn394 effectively hydrolyzed wheat straw, resulting in augmented volatile fatty acid production and ammonia-N levels during in vitro microbial fermentation. These findings indicate the potential of RuXyn394 as a novel and highly efficient enzyme preparation, offering promising prospects for the valorization of wheat straw, an agricultural by-product, in ruminant diets.

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Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition

Cited by 3 publications

References 65 publications

Microbiome engineering and ruminants

Microbiome engineering and ruminants

Exploring biomimetic potential of ruminant digestion strategies for lignocellulosic biomass utilization: A comprehensive review

Characterization of Novel Multifunctional Xylanase from Rumen Metagenome and Its Effects on In Vitro Microbial Fermentation of Wheat Straw

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