Characterization of a novel blaNDM-1-harbouring transposon coexisting with tmexCD2 -toprJ2 in an Aeromonas caviae isolate

Zhang, Luhua; Qiu, Yichuan; Fu, Yu; Li, Ying

doi:10.1016/j.jgar.2023.10.020

Cited by 1 publication

(1 citation statement)

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“…MARB in aquaculture are a serious global concern because they cannot only infect humans via direct transmission through the food chain but also transfer acquired antimicrobial resistance to human pathogens via MGEs [12]. A. caviae strain LZSFT54 showed resistance to gentamicin, tobramycin, meropenem, aztreonam, cefotaxime, ceftazidime, ceftriaxone, and ciprofloxacin, and it harbored a novel bla NDM-1 -bearing multidrug-resistance (MDR) transposon, containing dfrA12, bla OXA-18 , sul1 (two copies), and mph (A) [13]. Aeromonas spp.…”

Section: Introductionmentioning

confidence: 99%

A Novel Transposon Tn7709 Harbors Multidrug Resistance Genes in a Pathogenic Aeromonas media Strain QST31

Shang,

Li,

Zhang

et al. 2024

Microorganisms

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Pathogenic Aeromonas spp. are the etiological agents of Motile Aeromonas Septicemia (MAS). This study aimed to identify the pathogen of diseased tadpoles (Quasipaa spinosa) and the antibiotic-resistance characteristics of this bacterium. A Gram-negative bacterium, named strain QST31, was isolated from the ascites of diseased tadpoles and was identified as Aeromonas media based on physiological and biochemical tests, as well as molecular identification. Artificial infection experiments showed that strain QST31 was highly virulent to tadpoles, with an LC50 of 2.56 × 107 CFU/mL. The antimicrobial susceptibility of strain QST31 was evaluated using the disk diffusion method, and the results indicated that strain QST31 was resistant to 28 antibacterial agents. In addition, the whole genome of strain QST31 was sequenced, and the presence of antimicrobial resistance genes, integron, and transposon was investigated. Genes involved in adherence, hemolysis, type II secretion system (T2SS), T6SS, iron uptake system, and quorum sensing were identified in the genome of strain QST31. More than 12 antimicrobial resistance genes were predicted in the genome of strain QST31. Interestingly, a novel Tn7709 transposon harboring sul1, aadA16, catB3, blaOXA-21, aac(6′)-IIa, and tet(A) genes was identified. In conclusion, this is the first report on the isolation and identification of pathogenic A. media with multidrug resistance genes from diseased tadpoles. The results revealed that preventing and controlling aquatic animal diseases caused by multidrug resistance A. media will be a huge challenge in the future.

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Section: Introductionmentioning

confidence: 99%