Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology

Abstract

Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNγ and TNFα mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRγ gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.

Large granular lymphocyte lymphoma in the skin and urinary bladder of a dog

“…These tumors are commonly seen in the intestinal tract and have a poor prognosis since they are highly aggressive and malignant [7,19]. Few reports of canine LGL lymphoma have been reported, and these tumors are presumed to arise frequently from both the lymph nodes and the extranodal sites [3,5,6,10,17]. However, there have been no reports on canine LGL lymphoma concurrently located in the bladder and the skin.…”

“…In the present case, nimustine chemotherapy was initiated followed by combination therapy with vincristine and doxorubicin, and a relatively long survival of 356 days was observed. In dogs, the survival times of patients with intraperitoneal, mediastinal, pleural, pericardial, gastrointestinal, pituitary, and adrenal LGL lymphomas were less than 30 days despite undergoing treatments such as chemotherapy and surgery [2,3,10,12]. In contrast, relatively long survival times of 18 weeks and 195 days have been reported in cutaneous and spinal LGL lymphomas treated with CHOP-based protocol, respectively [6,16].…”

Large T‐cell extradural lymphoma with concurrent marked cerebrospinal fluid eosinophilia in a dog

“…31 Other anatomic locations reported include peripheral lymph nodes, mediastinum, effusions of the pericardium and pleura, bladder, and skin. [34][35][36][37][38] In 1 other case with suspected CNS involvement, LGL lymphoma was diagnosed via aspirates of the spleen and abdominal lymph node, with MRI indicating severe meningeal infiltrates; the neurologic status improved rapidly upon initiation of chemotherapy, and a survival of 195 days was reached. 39 On review of the other case reports and small case series of LGL lymphoma in dogs, neurologic involvement is not reported.…”

Analysis of endogenous and exogenous tumor upregulated promoter expression in canine tumors

“…MDCK and FDK were obtained from the American Type Culture Collection). Canine B cell lymphoma line 17-71 [45] (gift from Dr. Steven Suter, North Carolina State University) and peripheral T cell lymphoma line OSW [46] (gift from Dr. William C. Kisseberth, The Ohio State University) ( Table 1) were cultured in RPMI (Roswell Park Memorial Institute medium, Corning) with penicillin (100 IU/ml, Corning), streptomycin (100 ug/ml, Corning), amphotericin B (0.5ug/ml, Corning) and 10% FBS (Sigma). All cells were grown at 37˚C and 5% CO 2 and were validated to be of canine origin by species-specific PCR [47].…”