Characterization of a Novel Cr6+ Reducing Pseudomonas sp. with Plant Growth–Promoting Potential

Rajkumar, M.; Nagendran, R.; Lee, Kui Jae; Lee, Wang Hyu

doi:10.1007/s00284-005-4470-4

Cited by 93 publications

(45 citation statements)

References 23 publications

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“…The phytohormone IAA production offers great promise for sustaining the increased crop productivity. The metal resistant bacteria belonging to different genera such as Pseudomonas, Mycobacterium, Agrobacterium and Arthrobacter were found to have plant growth-promoting features that can potentially promote plants growth and reduce stress symptoms in plants (Dell'Amico et al, 2005;Rajkumar et al, 2005). The maximum plant growth promotion by P. jessenii PjM15 observed in the present study can be attributed to the solubilisation of phosphate and production of IAA.…”

Section: Discussionmentioning

confidence: 50%

“…The plates were incubated at 27°C for 48 h to screen resistant colonies. To check the extent of resistance, the selected bacterial isolates were grown in LB agar media containing different concentrations of Ni, Cu or Zn ranging from 100 to 1000 mg l À1 (Rajkumar et al, 2005). In order to isolate the PGPB, the heavy metal resistant strains were grown on DF salts minimal medium (Dworkin and Foster, 1958) supplemented with 3 mM ACC to provide a nitrogen source at 27°C for 168 h at 175 rpm.…”

Section: Isolation Of Metal Resistant-pgpbmentioning

confidence: 99%

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Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus communis in soil contaminated with heavy metals

2008

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The metal resistant-plant growth-promoting bacterial (PGPB) strains PsM6 and PjM15 isolated from a serpentine soil were characterized as Pseudomonas sp. and Pseudomonas jessenii, respectively, on the basis of their morphological, physiological, biochemical characteristics and 16S rDNA sequences. Assessment of plant growth-promoting parameters revealed the intrinsic ability of the strains for the utilization of 1-aminocyclopropane-1-carboxylic acid as the sole N source, solubilization of insoluble phosphate and production of indole-3-acetic acid (IAA). Further, a pot experiment was conducted to elucidate the effects of inoculating metal resistant PGPB on the plant growth and the uptake of Ni, Cu and Zn by Ricinus communis. Inoculation of Pseudomonas sp. PsM6 or P. jessenii PjM15 increased the shoot and root biomass of R. communis grown in non-contaminated and contaminated soil. However, the maximum biomass was observed in the plants inoculated with strain PjM15. This effect can be attributed to the solubilization of phosphate and production of IAA. Inoculation of Pseudomonas sp. PsM6 and PjM15 did not greatly alter the organ metal concentrations except Zn which concentration was higher in root, stem and leaf of inoculated plants. The results of metal extraction with PGPB strains showed that PsM6 was more efficient at solubilizing Zn than PjM15, and that PjM15 was better at solubilising Ni and Cu than PsM6. Owing to its wide action spectrum, the metal resistant PGPB could serve as an effective metal sequestering and growth-promoting bioinoculant for plants in metal-stressed soil. The present study has provided a new insight into the phytoremediation of metal-contaminated soil.

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Section: Discussionmentioning

confidence: 50%

Section: Isolation Of Metal Resistant-pgpbmentioning

confidence: 99%

Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus communis in soil contaminated with heavy metals

2008

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“…There are reports that have shown a high level tolerance to heavy metals by rhizobia (Wani and Khan, 2013). Conflicting reports are, however, available in the literature on the tolerance level of rhizobia, which could possibly be due to the variation in the tolerance ability of bacteria and growth conditions employed (Rajkumar et al, 2005). For instance, Rhizobium leguminosarum isolated from metal contaminated soil tolerated 92.9 μM of zinc (Delorme et al, 2003) while, Rhizobium species isolated from nodules of Trifolium repense tolerated 300 mg kgG 1 nickel and showed an effective symbiosis with its legume host, when grown in nickel amended soils (Smith and Giller, 1992).…”

Section: Resultsmentioning

confidence: 99%

Bioreduction of Cr (VI) by Heavy Metal Resistant Pseudomonas Species

Wani¹,

Ayoola²

2015

J. of Environmental Science and Technology

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Chromium (VI) contamination has accelerated due to rapid industrialization worldwide. Aim of this study was to check the bacterial species for their tolerance towards multiple metals, antibiotics and further check whether these bacteria are reducing Cr (VI). Bacterial strains were isolated from the industrial area situated at Lagos-Abeokuta Road, Ile Ise Awo, Abeokuta, Ogun State, Nigeria. All of the isolates showed tolerance to lead, zinc and chromium (VI). Bacterial species also showed tolerance towards antibiotics, 100% of strains were resistant to ampicillin, cotrimoxazole, colistin and gentamycin, 66.6% to nitrofurantoin and nalidixic acid, 83.3% were resistant to streptomycin whereas, 50% were resistant to tetracycline. Among all the strains, only two strains Pseudomonas strain PH2 and PH4 were chosen for chromium (VI) reduction as these strains showed maximum tolerance towards chromium (VI). Maximum reduction (60%) of chromium (VI) was observed at pH 6 by Pseudomonas spp. PH2, which was followed by pH 5 (50%) whereas, pH9 showed least reduction of 12.5%. Similarly, Pseudomonas spp. PH4 also reduced chromium considerably at pH 6 (60%), pH 5 (50%), pH 8 (37.5%) and at pH 9 (12.5%) respectively, at a concentration of 100 μg Cr mLG 1 after 120 h of incubation. The Pseudomonas species PH2 reduced chromium (VI) at concentration of 50 μg Cr mLG 1 (92%), 100 μg Cr mLG 1 (70%) and 150 μg Cr mLG 1 (50%), respectively at a pH of 6. Similarly, Pseudomonas species PH4 reduced chromium (VI) by 95% at 50 μg Cr mLG 1 , 70% at 100 μg Cr mLG 1 and 55% at 150 μg Cr mLG 1 at a pH of 6. Due to above properties strains could therefore, be used as bioremediators of metals in soils contaminated with heavy metals and can also increase the yield of various crops under heavy metal contamination.

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“…There are many reports which have shown varied level of tolerance by bacteria. This varied level of resistance could be due to the variation in growth conditions employed (Rajkumar et al, 2005). For example, Rhizobium leguminosarum have shown a tolerance level of 92.9 µM to zinc (Delorme et al, 2003) while Rhizobium species isolated from nodules of Trifolium repense tolerated 300 mg kgG nickel and when grown in nickel amended soil could 1 nodulate the legume crop effectively (Smith and Giller, 1992).…”

Section: Resultsmentioning

confidence: 99%

Chromium (Vi) Reduction by Streptococcus Species Isolated from the Industrial Area of Abeokuta, Ogun State, Nigeria

Wani¹,

Zainab²,

Wasiu³

et al. 2015

Research J. of Microbiology

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Chromium (VI) contamination has accelerated due to rapid industrialization worldwide. Aim of this study is to check the bacterial species for their tolerance towards multiple metals, antibiotics and plant growth promoting activity and further check whether these bacteria are reducing Cr (VI). Bacterial strains were isolated from metal contaminated soils of Abeokuta. All of the isolates showed tolerance to lead, zinc and chromium (VI). Bacterial specie also showed tolerance towards antibiotics, 100% of the isolates were tolerant to Septrin, Chloramphenicol, Sparfloxacin, Amoxicillin, Augmentin, Tarivid and Streptomycin, whereas 83.33% were tolerant to Gentamycin and Pefloxacin and 33.33% were resistant to Ciprofloxacin. All bacterial species were positive to ammonia, whereas strain PZ3 and PZ4 were found to be positive to HCN. Among all the strains, only Streptococcus spp. PZ4 showed reduction of Chromium (VI). Maximum reduction (85%) of chromium (VI) was observed at pH 7 by Streptococcus spp. PZ4. Similarly, Streptococcus spp. PZ4 reduced the chromium considerably at pH 5 (51.25%), pH 6 (72.5%), pH 8 (67.5%) and at pH 9 (45%), at a concentration of 100 µg Cr mLG after 120 h of incubation. Streptococcus spp. PZ4 1 reduced chromium (VI) at a concentration of 50 µg Cr mLG (47.5%), 100 µg Cr mLG (91.25%) and 1 1 150 µg Cr mLG (134.17%). Due to above properties strains could therefore be used a s 1 bioremediators of metals in soils contaminated with heavy metals and can also increase the yield of various crops under heavy metal contamination.

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Characterization of a Novel Cr6+ Reducing Pseudomonas sp. with Plant Growth–Promoting Potential

Cited by 93 publications

References 23 publications

Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus communis in soil contaminated with heavy metals

Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus communis in soil contaminated with heavy metals

Bioreduction of Cr (VI) by Heavy Metal Resistant Pseudomonas Species

Chromium (Vi) Reduction by Streptococcus Species Isolated from the Industrial Area of Abeokuta, Ogun State, Nigeria

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