Characterization of a novel dipeptidyl peptidase 1 of Trichinella spiralis and its participation in larval invasion

Yan, Shu Wei; Cheng, Yong Kang; Lu, Qi Qi; Zhang, Ru; Dan Liu, Ruo; Long, Shao Rong; Wang, Zhong Quan; Cui, Jing

doi:10.1016/j.actatropica.2023.107076

Acta Tropica

2024

DOI: 10.1016/j.actatropica.2023.107076

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Characterization of a novel dipeptidyl peptidase 1 of Trichinella spiralis and its participation in larval invasion

Shu Wei Yan,

Yong Kang Cheng,

Qi Qi Lu

et al.

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2024

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Cited by 3 publications

References 61 publications

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Trichinella spiralis C-type lectin mediates larva invasion of gut mucosa via binding to syndecan-1 and damaging epithelial integrity in mice

Wang,

Zhang,

Cong

et al. 2024

International Journal of Biological Macromolecules

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Trichinella spiralis C-type lectin mediates larva invasion of gut mucosa via binding to syndecan-1 and damaging epithelial integrity in mice

Wang,

Zhang,

Cong

et al. 2024

International Journal of Biological Macromolecules

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Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis

Cheng,

Zhang,

Zhang

et al. 2024

Parasite

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Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time, this transformation is accompanied by the oxidation-reduction of NAD(H) or NADP(H). This process not only helps to link amino acid metabolism with sugar metabolism, but also helps maintain the balance of intracellular pH and nitrogen homeostasis. In this study, a novel Trichinella spiralis glutamate dehydrogenase (TsGDH) was cloned, expressed and identified. The results revealed that TsGDH was expressed at various stages of development of the nematode T. spiralis, with higher expression levels in the adult worm stage, and was mainly localized in the cuticle, muscular layer, stichosome and female intrauterine embryos. After RNAi treatment, larval natural TsGDH enzyme activity was obviously reduced, and metabolism, molting, growth and reproduction were also significantly inhibited. The results indicate that TsGDH plays an important role in the development and survival of T. spiralis, and it may be a potential molecular target of anti-Trichinella vaccines and drugs.

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Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development

Li,

Lu,

Zheng

et al. 2024

Vet Res

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Long-chain fatty acid transport protein 1 (FATP1) is a member of the fatty acid transporter family. It facilitates transmembrane transport of fatty acids and participates in lipid metabolism. Lipids are essential components of the cell and organelle membranes of Trichinella spiralis. The nematode has lost the capacity to synthesise the necessary lipids de novo and has instead evolved to obtain fatty acids and their derivatives from its host. This study aims to ascertain the primary biological characteristics and roles of T. spiralis FATP1 (TsFATP1) in lipid metabolism, larval moulting, and the development of this nematode. The results show that TsFATP1 is highly expressed at enteral T. spiralis stages, mainly localised at the cuticle, the stichosome and the intrauterine embryos of the parasite. The silencing of the TsFATP1 gene by TsFATP1-specific dsRNA significantly decreases the expression levels of TsFATP1 in the worm. It reduces the contents of ATP, triglycerides, total cholesterol, and phospholipids both in vitro and in vivo. RNAi inhibits lipid metabolism, moulting, and the growth of this nematode. The results demonstrate that TsFATP1 plays an essential role in lipid metabolism, moulting, and the development of T. spiralis. It could also be a target candidate for the anti-Trichinella vaccine and drugs.

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Characterization of a novel dipeptidyl peptidase 1 of Trichinella spiralis and its participation in larval invasion

Cited by 3 publications

References 61 publications

Trichinella spiralis C-type lectin mediates larva invasion of gut mucosa via binding to syndecan-1 and damaging epithelial integrity in mice

Trichinella spiralis C-type lectin mediates larva invasion of gut mucosa via binding to syndecan-1 and damaging epithelial integrity in mice

Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis

Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development

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