Characterization of a novel DyP-type peroxidase from Streptomyces avermitilis

Sugawara, Kazuki; Nishihashi, Yuriko; Narioka, Tomomi; Yoshida, Toru; Morita, Mifumi; Sugano, Yasushi

doi:10.1016/j.jbiosc.2016.12.001

Cited by 32 publications

(31 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optimization of conditions for achieving higher levels of enzymatic activity is also important. DMP was used for those particular experiments, owing to its wide specificity as the substrate of oxidoreductases, laccases 41,42 , azoreductases 17,37,43 and peroxidases 22,24 . Ln 26 was able to partially decolorize the three dyes in liquid medium after around 3,5 days, although RBB was the most resistant one (Figure 1S).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye

Díez-Méndez

García‐Fraile

Solano

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Industrial synthetic dyes cause health and environmental problems. This work describes the isolation of 84 bacterial strains from the midgut of the Lasius niger ant and the evaluation of their potential application in dye bioremediation. Strains were identified and classified as judged by rRNA 16S. The most abundant isolates were found to belong to Actinobacteria (49%) and Firmicutes (47.2%). We analyzed the content in laccase, azoreductase and peroxidase activities and their ability to degrade three known dyes (azo, thiazine and anthraquinone) with different chemical structures. Strain Ln26 (identified as Brevibacterium permense) strongly decolorized the three dyes tested at different conditions. Strain Ln78 (Streptomyces ambofaciens) exhibited a high level of activity in the presence of Toluidine Blue (TB). It was determined that 8.5 was the optimal pH for these two strains, the optimal temperature conditions ranged between 22 and 37 °C, and acidic pHs and temperatures around 50 °C caused enzyme inactivation. Finally, the genome of the most promising candidate (Ln26, approximately 4.2 Mb in size) was sequenced. Genes coding for two DyP-type peroxidases, one laccase and one azoreductase were identified and account for the ability of this strain to effectively oxidize a variety of dyes with different chemical structures.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the use of peroxides to remove azo dyes and other xenobiotic compounds from textile effluents is well established 23 . These enzymes are presented in both eukaryotic and prokaryotic organisms 15 and new microbial peroxidases have also been recently described 24 .…”

Section: Introductionmentioning

confidence: 99%

The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye

Díez-Méndez

García‐Fraile

Solano

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This heme-dependent peroxidase, AnaPX, showed substantial activity on phenolic compounds, such as guaiacol and pyrogallol, and this was significantly increased 50-fold by the redox mediator syringaldehyde. Recently, another bacterial DyP within the C class was discovered from Streptomyces avermitilis , following AnaPX and DyP2 (Sugawara et al, 2017). Even though SaDyP2 was found to be active on a typical oxidation indicator, further research would be required to determine the actual activity of lignin modification.…”

Section: Lignin Depolymerization To Produce Low-molecular-weight Phenmentioning

confidence: 99%

Bacterial Valorization of Lignin: Strains, Enzymes, Conversion Pathways, Biosensors, and Perspectives

Lee

Kang

Bae

et al. 2019

Front. Bioeng. Biotechnol.

147

View full text Add to dashboard Cite

Lignin, an aromatic polymer found in plants, has been studied for years in many biological fields. Initially, when biofuel was produced from lignocellulosic biomass, lignin was regarded as waste generated by the biorefinery and had to be removed, because of its inhibitory effects on fermentative bacteria. Although it has since proven to be a natural resource for bio-products with considerable potential, its utilization is confined by its complex structure. Hence, the microbial degradation of lignin has attracted researchers' interest to overcome this problem. From this perspective, the studies have primarily focused on fungal systems, such as extracellular peroxidase and laccase from white- and brown-rot fungi. However, recent reports have suggested that bacteria play an increasing role in breaking down lignin. This paper, therefore, reviews the role of bacteria in lignin and lignin-related research. Several reports on bacterial species in soil that can degrade lignin and their enzymes are included. In addition, a cellulolytic anaerobic bacterium capable of solubilizing lignin and carbohydrate simultaneously has recently been identified, even though the enzyme involved has not been discovered yet. The assimilation of lignin-derived small molecules and their conversion to renewable chemicals by bacteria, such as muconic acid and polyhydroxyalkanoates, including genetic modification to enhance their capability was discussed. This review also covers the indirect use of bacteria for lignin degradation, which is concerned with whole-cell biosensors designed to detect the aromatic chemicals released from lignin transformation.

show abstract

“…2014; Rahmanpour and Bugg 2015; Sugawara et al . 2017) with prospect in remediation of environmental phenolic pollutants. Dye decolourizing peroxidases from Bacillus and Pseudomonas species have shown ability to oxidize phenolic and non‐phenolic lignin units (Santos et al .…”

Section: Resultsmentioning

confidence: 99%

Emerging biotechnological potentials of DyP‐type peroxidases in remediation of lignin wastes and phenolic pollutants: a global assessment (2007–2019)

Falade

Ekundayo

2020

Letters Applied Microbiology

View full text Add to dashboard Cite

Significance and Impact of the Study: Dye decolourizing peroxidase (DyP)-type peroxidases are versatile microbial enzymes with inherent aptitude for decolourization of recalcitrant synthetic dyes. Interestingly, this study brought to the fore, the emerging potentials of DyP-type peroxidases in lignin degradation and remediation of phenolic pollutants. There is a global increasing trend in research on DyP-type peroxidases with greater research output from advanced economics compared to low and middle-income countries (LMIC) and very low intercountry collaborations. Despite the biotechnological prospects of DyP-type peroxidases, the enzyme is currently underexplored globally, hence, it is hoped that this study will open the eyes of science community to this emerging field of research.

show abstract

Characterization of a novel DyP-type peroxidase from Streptomyces avermitilis

Cited by 32 publications

References 28 publications

The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye

The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye

Bacterial Valorization of Lignin: Strains, Enzymes, Conversion Pathways, Biosensors, and Perspectives

Emerging biotechnological potentials of DyP‐type peroxidases in remediation of lignin wastes and phenolic pollutants: a global assessment (2007–2019)

Contact Info

Product

Resources

About