Characterization of a novel facultative <i>Methylocystis</i> species capable of growth on methane, acetate and ethanol

Im, Jeongdae; Lee, Sung Woo; Yoon, Sukhwan; DiSpirito, Alan A.; Semrau, Jeremy D.

doi:10.1111/j.1758-2229.2010.00204.x

Cited by 88 publications

(58 citation statements)

References 39 publications

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“…These microorganisms are capable of fixing nitrogen and consuming CH 4 at both high and low concentrations, and a few strains are able to use acetate as an additional carbon source. The versatile metabolic capabilities of type II methanotrophs possibly represent an important part of their survival strategy in oligotrophic peatlands [12,37,38]. The deficiency of soil TOC and TN in Sanjiang wetland may create an oligotrophic environment which supports the growth of abundant Methylocystis.…”

Section: Discussionmentioning

confidence: 99%

Aerobic Methanotroph Diversity in Sanjiang Wetland, Northeast China

et al. 2014

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Aerobic methanotrophs present in wetlands can serve as a methane filter and thereby significantly reduce methane emissions. Sanjiang wetland is a major methane source and the second largest wetland in China, yet little is known about the characteristics of aerobic methanotrophs in this region. In the present study, we investigated the diversity and abundance of methanotrophs in marsh soils from Sanjiang wetland with three different types of vegetation by 16S ribosomal RNA (rRNA) and pmoA gene analysis. Quantitative polymerase chain reaction analysis revealed the highest number of pmoA gene copies in marsh soils vegetated with Carex lasiocarpa (10(9) g(-1) dry soil), followed by Carex meyeriana, and the least with Deyeuxia angustifolia (10(8) g(-1) dry soil). Consistent results were obtained using Sanger sequencing and pyrosequencing techniques, both indicating the codominance of Methylobacter and Methylocystis species in Sanjiang wetland. Other less abundant methanotrophy, including cultivated Methylomonas and Methylosinus genus, and uncultured clusters such as LP20 and JR-1, were also detected in the wetland. Methanotroph diversity was almost the same in three different vegetation covered soils, suggesting that vegetation types had very little influence on the methanotroph diversity. Our study gives an in-depth insight into the community composition of aerobic methanotrophs in the Sanjiang wetland.

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Section: Discussionmentioning

confidence: 99%

Aerobic Methanotroph Diversity in Sanjiang Wetland, Northeast China

et al. 2014

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“…3). As Methylocystis strain SB2 can express only pMMO (38), which requires copper for high activity, it may be that MB from M. trichosporium OB3b increased the bioavailability of copper, thereby increasing the activity of pMMO in Methylocystis strain SB2. If so, this suggests that MB made by one methanotroph may actually be taken up by others (as also suggested by the mercury uptake data [ Table 1]).…”

Section: Discussionmentioning

confidence: 99%

Detoxification of Mercury by Methanobactin from Methylosinus trichosporium OB3b

Vorobev

Jagadevan

Baral

et al. 2013

Appl Environ Microbiol

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bMany methanotrophs have been shown to synthesize methanobactin, a novel biogenic copper-chelating agent or chalkophore. Methanobactin binds copper via two heterocyclic rings with associated enethiol groups. The structure of methanobactin suggests that it can bind other metals, including mercury. Here we report that methanobactin from Methylosinus trichosporium OB3b does indeed bind mercury when added as HgCl 2 and, in doing so, reduced toxicity associated with Hg(II) for both Alphaproteobacteria methanotrophs, including M. trichosporium OB3b, M. trichosporium OB3b ⌬mbnA (a mutant defective in methanobactin production), and Methylocystis sp. strain SB2, and a Gammaproteobacteria methanotroph, Methylomicrobium album BG8. Mercury binding by methanobactin was evident in both the presence and absence of copper, despite the fact that methanobactin had a much higher affinity for copper due to the rapid and irreversible binding of mercury by methanobactin. The formation of a gray precipitate suggested that Hg(II), after being bound by methanobactin, was reduced to Hg(0) but was not volatilized. Rather, mercury remained associated with methanobactin and was also found associated with methanotrophic biomass. It thus appears that although the mercury-methanobactin complex was cell associated, mercury was not removed from methanobactin. The amount of biomass-associated mercury in the presence of methanobactin from M. trichosporium OB3b was greatest for M. trichosporium wild-type strain OB3b and the ⌬mbnA mutant and least for M. album BG8, suggesting that methanotrophs may have selective methanobactin uptake systems that may be based on TonB-dependent transporters but that such uptake systems exhibit a degree of infidelity.

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“…Within the class Alphaproteobacteria, the genera Methylosinus and Methylocystis are found in the family Methylocystaceae and the genera Methylocella, Methyloferula, and Methylocapsa in the family Beijerinkiaceae. In the last 15 years, there have been increasing reports of facultative methanotrophs within the Methylocella, Methylocapsa, and Methylocystis genera that can use multicompounds for growth in addition to methane (22)(23)(24)(25)(26). Also known today are filamentous methanotrophs from other genera, such as Crenothrix and Clonothrix, and nonproteobacterial (verrucomicrobial) methanotrophs of the genus Methylacidiphilum growing at high temperatures and low pH have also been discovered recently (27).…”

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Methanobactin and the Link between Copper and Bacterial Methane Oxidation

DiSpirito

Semrau

Murrell

et al. 2016

Microbiol Mol Biol Rev

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SUMMARYMethanobactins (mbs) are low-molecular-mass (<1,200 Da) copper-binding peptides, or chalkophores, produced by many methane-oxidizing bacteria (methanotrophs). These molecules exhibit similarities to certain iron-binding siderophores but are expressed and secreted in response to copper limitation. Structurally, mbs are characterized by a pair of heterocyclic rings with associated thioamide groups that form the copper coordination site. One of the rings is always an oxazolone and the second ring an oxazolone, an imidazolone, or a pyrazinedione moiety. The mb molecule originates from a peptide precursor that undergoes a series of posttranslational modifications, including (i) ring formation, (ii) cleavage of a leader peptide sequence, and (iii) in some cases, addition of a sulfate group. Functionally, mbs represent the extracellular component of a copper acquisition system. Consistent with this role in copper acquisition, mbs have a high affinity for copper ions. Following binding, mbs rapidly reduce Cu2+to Cu1+. In addition to binding copper, mbs will bind most transition metals and near-transition metals and protect the host methanotroph as well as other bacteria from toxic metals. Several other physiological functions have been assigned to mbs, based primarily on their redox and metal-binding properties. In this review, we examine the current state of knowledge of this novel type of metal-binding peptide. We also explore its potential applications, how mbs may alter the bioavailability of multiple metals, and the many roles mbs may play in the physiology of methanotrophs.

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Characterization of a novel facultative Methylocystis species capable of growth on methane, acetate and ethanol

Cited by 88 publications

References 39 publications

Aerobic Methanotroph Diversity in Sanjiang Wetland, Northeast China

Aerobic Methanotroph Diversity in Sanjiang Wetland, Northeast China

Detoxification of Mercury by Methanobactin from Methylosinus trichosporium OB3b

Methanobactin and the Link between Copper and Bacterial Methane Oxidation

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