Characterization of a novel genomovirus in the phytopathogenic fungus Botrytis cinerea

A Mechanically Transmitted DNA Mycovirus Is Targeted by the Defence Machinery of Its Host, Botrytis cinerea

“…The maximum likelihood (ML) tree based on the Rep sequences of B. cinerea extra-chromosomal DNA elements and other selected circular ssDNA sequences ( Figure 2 ) revealed that Sequence A and B were closely related to, but distinct from, sequences of Genomoviridae . Using the taxonomic framework for the classification of genomoviruses based on the sequence information [ 8 ] and as suggested by the ML phylogenetic analysis, the extra-chromosomal DNA elements identified in this study probably represent a novel viral species of a previously unrecognised genus in the family Genomoviridae as recently suggested for BcGV1 [ 26 ]. Following the basis of the nomenclature of Genomoviridae genera [ 8 ], the name Gemydayirivirus was proposed for the newly created genus, to accommodate the B. cinerea extra-chromosomal DNA elements of this study, which we tentatively named BGDaV1 (Sequence A) and BGDaV2 (Sequence B).…”

“…The exact position of a putative nonanucleotide sequence motif could not be confirmed. Possible positions and sequences of the putative nonanucleotide motifs include CTATAACATC (current nt position: 1–10) as compared to that of Sequence A (CTATCAACAC), TAACAGTACC as compared to the putative nonanucleotide motif sequence of Botrytis cinerea genomovirus 1 (BcGV1-LD17-26) (TAACAGTACC; nt position: 113–122) [ 26 ] or TAATTAATTC as compared to the putative nonanucleotide motif sequence of Botrytis cinerea ssDNA virus 1 (BcSDV1-BCS11_DN160) (TAATTAATTC; nt position: 1132–1141) [ 27 ].…”

“…A BLASTp search of the translated ORF I sequence against the NCBI database revealed that Sequence A and B share the highest aa sequence identities with BcGV1-LD17-26 (91.9 and 99.38%) and BcSDV1-BCS11_DN160 (91.9 and 98.75%), respectively [ 26 , 27 ]. These proteins also had ~39 and ~40% similarity with Reps of black robin-associated gemykibivirus 1 (nt sequence accession no: KF371634) detected in New Zealand [ 28 ] and dragonfly-associated gemykibivirus 1 (nt sequence accession no: JX185430) identified in USA [ 29 ], respectively.…”

“…The Cap proteins of a virus together assemble to encapsidate the viral genome and are often highly diverse among virus families, but in most cases, Caps encoded by divergent CRESS-DNA viruses share no homology [ 33 ]. Putative Cap-encoding genes were not identifiable in the BGDaV1 sequence, as neither ORF II (Sequence A and B) nor III (Sequence A) shared any similarities with known CRESS DNA virus Caps apart from the hypothetical proteins encoded by ORF II of BcGV1 [ 26 ], which shares ~93% aa identity with the proteins encoded by ORF II of BGDaV1 and BGDaV2. As for BGDaV1, CRESS DNA viruses with two unknown ORFs and no detectable putative Cap genes have been previously reported, such as McMurdo Ice Shelf pond-associated circular DNA virus-4 and -8 [ 37 ] and sewage-associated circular DNA virus-31 [ 33 ].…”

Study on the occurrence law and green control of grape gray mold from the perspective of ecological balance

“…After the initial infection, many conidia will grow and infect again under the action of airflow. Other people's studies show that gray mold resistance genes are controlled by polygenic loci, but to achieve fine location of gray mold resistance loci, it is necessary to carry out multi-point resistance identification for many years and analyze that exist stably in multiple environments [18]. Mancozeb is an excellent protective fungicide, which is a lowtoxic pesticide.…”