Characterization of a novel marine origin aerobic nitrifying–denitrifying bacterium isolated from shrimp culture ponds

Nucleotides, nucleosides and nucleic acids (NU) have many critical functions in supporting life and increasing evidence suggests that exogenous supply can benefit the health of mammals and fish. For these reasons, a 6-week feeding trial was conducted on juvenile European seabass (Dicentrarchus labrax) with diets containing 0%, 0.15% and 0.3% inclusion of a NU mixture (Laltide ® ) derived from the yeast Saccharomyces cerevisiae. At the end of the study no significant differences were found in fish performance, although a tendency towards better performance was indicated in fish fed the Nu0.3 diet. In relation to histological assessment, a significantly greater perimeter ratio; internal to outer (IP/OP) was observed in the posterior intestine of fish fed supplemental NU. Microvilli heights in the posterior intestine were also shown to be significantly promoted in fish fed NU diets (p < 0.05). Goblet cell abundance was shown to be unaffected by the inclusion of NU in the diet (p > 0.05). Overall, this study indicates that orally administered NU may be effective promoters of gut functional topography with marginal associated improvements to fish performance.Nonetheless, longer exposure and/or commercial scale application, and in diets that were challenging in use of high inclusion levels of plant by-products would potentially amplify improvements in production characteristics, in turn benefiting fish culturists. K E Y W O R D Saquaculture, intestine, microvilli, morphology, nucleosides, nucleotides

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“…The data that support the findings of this study are openly available in figshare at https://doi.org/10.6084/m9.figshare.7873178.v2 (Zhao, ).…”

Section: Data Availabilitysupporting

confidence: 67%

Dietary nucleotides enhance growth performance, feed efficiency and intestinal functional topography in European Seabass ( Dicentrarchus labrax )

et al. 2019

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“…The optimal C/N ratio of AB15 and NB14 is 15 and 10, respectively, and under this condition, the corresponding ammonia and nitrite degradation rates exceed 80 and 85%, respectively. Several reports also found optimum C/N ratio 10 in the Vibrio diabolicus SF16 (Duan et al, 2015) and in Pseudomonas stutzeri strain XL‐2(Zhao et al, 2018), and C/N ratio 15 in Bacillus subtilis strain H1(Zhao, Tian, Li, Dong, & Jiang, 2019), and in Marinobacter sp. F6 (Zheng et al, 2012).…”

Section: Discussionmentioning

confidence: 90%

“…Several reports also found optimum C/N ratio 10 in the Vibrio diabolicus SF16 (Duan et al, 2015) and in Pseudomonas stutzeri strain XL-2 (Zhao et al, 2018), and C/N ratio 15 in Bacillus subtilis strain H1 (Zhao, Tian, Li, Dong, & Jiang, 2019), and in Marinobacter sp. F6 (Zheng et al, 2012).…”

Section: Isolation and Identification Of Strainsmentioning

confidence: 89%

Characterization of Vibrio sp. strain AB15 and Pseudomonas fluorescens strain NB14 from the biofloc of shrimp culture ponds capable of high ammonia and nitrite removal efficiency

Dou

Chen

Pan

et al. 2021

J World Aquaculture Soc

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The development of intensive aquaculture industry has aggravated the dangers of ammonia and nitrite in aquatic ecosystems. Probiotics have been widely used to maintain and improve water quality by removal of ammonia and nitrite in aquaculture. In this study, two facultative anaerobic strains AB15 and NB14, capable of efficient ammonia and nitrite removal, were isolated from biofloc, which acts as a natural micro‐flora storeroom in shrimp culture ponds, and were identified as Vibrio sp. and Pseudomonas fluorescens, respectively. The removal efficiency of ammonia and nitrite by AB15 and NB14 was 96.8 and 89.6%, respectively. The optimum conditions of AB15 and NB14 were as follows: temperature (25°C vs. 30°C), salinity (25‰ both), initial pH (7.5 vs. 8.0), C/N ratio (15 vs. 10). The two strains had great performance on inhibiting the common pathogens (Vibrio harveyi, Vibrio splendidus, Vibrio anguillarum) in aquaculture at concentrations of 105–108 CFU/mL. Biosecurity assessment indicated that both AB15 and NB14 were harmless for survival and could be beneficial for the growth and immune enhancement of Litopenaeus vannamei. All results demonstrate that the two isolated strains have high promise for future applications of removing ammonia and nitrite and maintaining biosecurity in shrimp culture ponds.

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“…By contrast, freshwater-derived HNAD (FHNAD) bacteria were significantly inhibited at 15 g·L –1 NaCl . Although MHNAD bacteria have a good potential to treat high-salinity wastewaters, previous studies were mostly conducted with NaCl-simulated saline wastewaters in shake flasks. , …”

Section: Introductionmentioning

confidence: 99%

Marine Heterotrophic Nitrification–Aerobic Denitrification Process Treating Mariculture Wastewater: Microbiome and Resistome Responses to Multiantibiotic Stresses

Wang,

Li,

Dong

et al. 2023

ACS EST Water

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Efficient treatment of mariculture wastewater is essential to the sustainable development of the mariculture industry, yet a high salinity and multiple antibiotics contained therein pose a serious challenge to biological treatment. In this study, marine heterotrophic nitrification–aerobic denitrification (MHNAD) bacteria were successfully augmented for mariculture wastewater treatment, and their multiantibiotic (i.e., ampicillin-tetracycline-sulfamethoxazole (ATS)) resistance mechanisms were deciphered through the co-occurrence patterns of microbiome and resistome. Up to an ATS dose of 36 mg·L–1, MHNAD bacteria exhibited a strong antibiotic resistance, achieving high organic and nitrogen removal efficiencies of 95.2 and 100%, respectively. Meanwhile, more extracellular polymeric substances were produced to enhance the bacterial resistance to antibiotics. The MHNAD genus, Klebsiella, invariably dominated the bioreactor (mean relative abundance of 34.1%) and mainly accounted for pollutant removals. The absolute abundance of total antibiotic resistance genes increased by 21.7 folds at an ATS dose of 72 mg·L–1, as compared to that without ATS. The abundance of sul1, tetQ, or intI1 was positively correlated with the ATS dose, and Klebsiella was strongly correlated with sul1, tetQ, tetX, and bla TEM. This study proposes a novel process for mariculture wastewater treatment through augmentation of MHNAD bacteria under multiantibiotic stresses.

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Characterization of a novel marine origin aerobic nitrifying–denitrifying bacterium isolated from shrimp culture ponds

Cited by 21 publications

References 50 publications

Dietary nucleotides enhance growth performance, feed efficiency and intestinal functional topography in European Seabass ( Dicentrarchus labrax )

Dietary nucleotides enhance growth performance, feed efficiency and intestinal functional topography in European Seabass ( Dicentrarchus labrax )

Characterization of Vibrio sp. strain AB15 and Pseudomonas fluorescens strain NB14 from the biofloc of shrimp culture ponds capable of high ammonia and nitrite removal efficiency

Marine Heterotrophic Nitrification–Aerobic Denitrification Process Treating Mariculture Wastewater: Microbiome and Resistome Responses to Multiantibiotic Stresses

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