Characterization of a Novel Myrosinase with High Activity from Marine Bacterium Shewanella baltica Myr-37

Ye, Qinwen; Fang, Yiwu; Li, Mengjiao; Mi, Haoyu; Liu, Shu; Yang, Guang; Lu, Jing; Zhao, Yaling; Liu, Qitong; Zhang, Wei; Hou, Xiaoyue

doi:10.3390/ijms231911258

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…50 However, another study reported that MYR activity was decreased by adding both Zn 2+ and other metal ions such as Mg 2+ , Ag + and Fe 3+ . 51 In our results, we found that 0.5-10 mmol L −1 Zn 2+ decreased the total ITCs content in PRSP extract (see Supporting information, Fig. S3), suggesting that the properties of MYR are slightly different depending on the sources.…”

Section: Production Of Optimal Prsp Extractmentioning

confidence: 52%

“…Especially, Zn 2+ has been reported to activate MYR activity in broccoli seed 49 or contribute to increasing the stability of SFEN in radish seed 50 . However, another study reported that MYR activity was decreased by adding both Zn 2+ and other metal ions such as Mg 2+ , Ag + and Fe 3+ 51 . In our results, we found that 0.5–10 mmol L −1 Zn 2+ decreased the total ITCs content in PRSP extract (see Supporting information, Fig.…”

Section: Resultsmentioning

confidence: 99%

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Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content

Hur,

Lee,

Cho

et al. 2024

J Sci Food Agric

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BackgroundCruciferous vegetable sprout has been highlighted as a promising functional material rich in bioactive compounds called isothiocyanates (ITCs), and it can be grown in very short periods in controlled indoor farms. However, since ITCs content depends on multiple factors such as cultivar, germination time, and myrosinase activity, those variables need to be controlled during germination or extraction to produce functional materials enriched in ITCs. Sulforaphene (SFEN), an ITC found primarily in radishes (Raphanus sativus L.), exerts beneficial effects on obesity. However, the optimal germination and extraction conditions for radish sprout (RSP) to increase SFEN content remain unascertained, and the extract's anti‐obesity effect has yet to be evaluated.ResultsThis study found that the SFEN content was highest in purple radish sprout (PRSP) among the 6 cultivars investigated. Optimal SFEN content occurred after 2 days of PRSP germination (2‐d PRSP). To maximize the dry matter yield, total ITCs, and SFEN contents in RSP extract, we found the optimal conditions for extracting PRSP [27.5 °C, 60 min, 1:75.52 solute/solvent (w/v), no ascorbic acid] by using response surface methodology. Consistent with high SFEN content, 2‐d PRSP extract significantly outperformed 3‐d or 4‐d PRSP extract in inhibiting lipid accumulation in 3T3‐L1 cells. Moreover, 2‐d PRSP extract suppressed adipogenesis and lipogenesis–related protein expression.ConclusionRegarding the cultivar, germination time, and extraction conditions, optimally produced PRSP extract contains high SFEN content and exerts anti‐obesity effects. Thus, we suggest PRSP extract as a potent functional material for obesity prevention.This article is protected by copyright. All rights reserved.

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Section: Production Of Optimal Prsp Extractmentioning

confidence: 52%

Section: Resultsmentioning

confidence: 99%

Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content

Hur,

Lee,

Cho

et al. 2024

J Sci Food Agric

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“…The aliphatic GLSs of Col-0 had no effect on leaf bacterial community composition and in vitro growth on 4MSOB-GLS was slow and relatively poor. This could be because it was not degraded by the bacterial myrosinase, which are known to show substrate specificity ( 45 , 48 ). The success of R3 might also depend on formation and degradation of ITCs during bacterial GLS metabolism.…”

Section: Discussionmentioning

confidence: 99%

Beyond defense: Glucosinolate structural diversity shapes recruitment of a metabolic network of leaf-associated bacteria

Unger,

Raza,

Mayer

et al. 2023

Preprint

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Leaf bacteria are critical for plant health, but little is known about how plant traits control their recruitment. Aliphatic glucosinolates (GLSs) are secondary metabolites present in leaves of Brassicaceae plants in genotypically-defined mixtures. Upon damage, they are broken down to products that deter herbivory and inhibit pathogens. Using twoA. thalianagenotypes with different aliphatic GLS profiles, we find that structural variants differentially affect commensal leaf bacteria: In the model genotype Col-0, GLS breakdown products (mostly from 4-methylsulfinylbutyl-glucosinolate) are potentially highly toxic to bacteria but have no effect on natural leaf colonization. In contrast, in anA. thalianagenotype from a wild population, GLS (mostly allyl-GLS) enriches Burkholderiales bacteria, an effect also detected in nature. Indeed,in-vitroas a carbon source, intact allyl-GLS specifically enriches a Burkholderiales-containing community in which Burkholderiales depend on other bacteria but in turn increase community growth rates. Metabolism of different GLSs is linked to breakdown product detoxification, helping explain GLS structural control of community recruitment.

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“…Meanwhile, Sangkret et al found that the main elements affecting SFN production were myrosinase activity, temperature, pH, and reaction time [ 14 ]. Recently, researchers obtained a new myrosinase-producing bacterium from marine sediment (Marine Bacterium Shewanella baltica Myr-37) [ 58 ]. Once the reaction temperature is 40 °C and pH = 7.0, myrosinase can efficiently degrade sulforaphane to SFN in 25 min, the yield is 0.57 mg/mL, and the corresponding SFN conversion efficiency is 89%.…”

Section: Intestinal Microorganisms Can Enhance the Utilization Of Sfnmentioning

confidence: 99%

Microorganisms—An Effective Tool to Intensify the Utilization of Sulforaphane

Wang²,

Zhao³

et al. 2022

Foods

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Sulforaphane (SFN) was generated by the hydrolysis of glucoraphanin under the action of myrosinase. However, due to the instability of SFN, the bioavailability of SFN was limited. Meanwhile, the gut flora obtained the ability to synthesize myrosinase and glucoraphanin, which could be converted into SFN in the intestine. However, the ability of microorganisms to synthesize myrosinase in the gut was limited. Therefore, microorganisms with myrosinase synthesis ability need to be supplemented. With the development of research, microorganisms with high levels of myrosinase synthesis could be obtained by artificial selection and gene modification. Researchers found the SFN production rate of the transformed microorganisms could be significantly improved. However, despite applying transformation technology and regulating nutrients to microorganisms, it still could not provide the best efficiency during generating SFN and could not accomplish colonization in the intestine. Due to the great effect of microencapsulation on improving the colonization ability of microorganisms, microencapsulation is currently an important way to deliver microorganisms into the gut. This article mainly analyzed the possibility of obtaining SFN-producing microorganisms through gene modification and delivering them to the gut via microencapsulation to improve the utilization rate of SFN. It could provide a theoretical basis for expanding the application scope of SFN.

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Characterization of a Novel Myrosinase with High Activity from Marine Bacterium Shewanella baltica Myr-37

Cited by 8 publications

References 34 publications

Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content

Optimization of cultivar, germination time and extraction for radish sprout extract with high sulforaphene content

Beyond defense: Glucosinolate structural diversity shapes recruitment of a metabolic network of leaf-associated bacteria

Microorganisms—An Effective Tool to Intensify the Utilization of Sulforaphane

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