Characterization of a novel RNAi yeast insecticide that silences mosquito 5-HT1 receptor genes

Mysore, Keshava; Njoroge, Teresia M.; Stewart, Akilah T. M.; Winter, Nikhella; Hamid-Adiamoh, Majidah; Sun, Longhua; Feng, Rachel Shui; James, Lester D.; Mohammed, Azad; Severson, David W.; Duman-Scheel, Molly

doi:10.1038/s41598-023-49799-3

Cited by 2 publications

(1 citation statement)

References 69 publications

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“…Labeled dsRNA injected into females was found enriched mainly in hemocytes and ovaries [71], but could not be detected after oral administration. On the contrary, many solid studies show high RNAi efficiency upon the oral administration of naked iRNAs [31,33,43,72,73].…”

Section: Discussionmentioning

confidence: 99%

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Figueiredo Prates,

Fiebig,

Schlosser

et al. 2024

IJMS

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In this study, we report the complexities and challenges associated with achieving robust RNA interference (RNAi)-mediated gene knockdown in the mosquitoes Aedes aegypti and Aedes albopictus, a pivotal approach for genetic analysis and vector control. Despite RNAi’s potential for species-specific gene targeting, our independent efforts to establish oral delivery of RNAi for identifying genes critical for mosquito development and fitness encountered significant challenges, failing to reproduce previously reported potent RNAi effects. We independently evaluated a range of RNAi-inducing molecules (siRNAs, shRNAs, and dsRNAs) and administration methods (oral delivery, immersion, and microinjection) in three different laboratories. We also tested various mosquito strains and utilized microorganisms for RNA delivery. Our results reveal a pronounced inconsistency in RNAi efficacy, characterized by minimal effects on larval survival and gene expression levels in most instances despite strong published effects for the tested targets. One or multiple factors, including RNase activity in the gut, the cellular internalization and processing of RNA molecules, and the systemic dissemination of the RNAi signal, could be involved in this variability, all of which are barely understood in mosquitoes. The challenges identified in this study highlight the necessity for additional research into the underlying mechanisms of mosquito RNAi to develop more robust RNAi-based methodologies. Our findings emphasize the intricacies of RNAi application in mosquitoes, which present a substantial barrier to its utilization in genetic control strategies.

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Section: Discussionmentioning

confidence: 99%

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Figueiredo Prates,

Fiebig,

Schlosser

et al. 2024

IJMS

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The role of G protein‐coupled receptors and their ligands in animal domestication

Kleinau,

Chini,

Andersson

et al. 2024

Animal Genetics

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The domestication of plants and animals has resulted in one of the most significant cultural and socio‐economical transitions in human history. Domestication of animals, including human‐supervised reproduction, largely uncoupled particular animal species from their natural, evolutionary history driven by environmental and ecological factors. The primary motivations for domesticating animals were, and still are, producing food and materials (e.g. meat, eggs, honey or milk products, wool, leather products, jewelry and medication products) to support plowing in agriculture or in transportation (e.g. horse, cattle, camel and llama) and to facilitate human activities (for hunting, rescuing, therapeutic aid, guarding behavior and protecting or just as a companion). In recent years, decoded genetic information from more than 40 domesticated animal species have become available; these studies have identified genes and mutations associated with specific physiological and behavioral traits contributing to the complex genetic background of animal domestication. These breeding‐altered genomes provide insights into the regulation of different physiological areas, including information on links between e.g. endocrinology and behavior, with important pathophysiological implications (e.g. for obesity and cancer), extending the interest in domestication well beyond the field. Several genes that have undergone selection during domestication and breeding encode specific G protein‐coupled receptors, a class of membrane‐spanning receptors involved in the regulation of a number of overarching functions such as reproduction, development, body homeostasis, metabolism, stress responses, cognition, learning and memory. Here we summarize the available literature on variations in G protein‐coupled receptors and their ligands and how these have contributed to animal domestication.

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Characterization of a novel RNAi yeast insecticide that silences mosquito 5-HT1 receptor genes

Cited by 2 publications

References 69 publications

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

Challenges of Robust RNAi-Mediated Gene Silencing in Aedes Mosquitoes

The role of G protein‐coupled receptors and their ligands in animal domestication

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