Characterization of a novel thermophilic, cellulose-degrading bacterium Paenibacillus sp. strain B39

Abstract

Aims: The aims of this study were to identify and characterize the novel thermophilic, cellulose‐degrading bacterium Paenibacillus sp. strain B39. Methods and Results: Strain B39 was closely related to Paenibacillus cookii in 16S rRNA gene sequence. Nonetheless, this isolate can be identified as a novel Paenibacillus sp. with respect to its physiological characteristics, biochemical reactions, and profiles of fatty acid compositions. A cellulase with both CMCase and avicelase activities was secreted from strain B39 and purified by ion‐exchange chromatography. By sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis, the molecular weight of B39 cellulase was determined as 148 kDa, which was much higher than other cellulases currently reported from Paenibacillus species. The enzyme showed a maximum CMCase activity at 60°C and pH 6·5. Addition of 1 mmol l−1 of Ca2+ markedly enhanced both CMCase and avicelase activities of the enzyme. Conclusions: We have identified and characterized a novel thermophilic Paenibacillus sp. strain B39 which produced a high‐molecular weight cellulase with both CMCase and avicelase activities. Significance and Impact of the Study: Based on the ability to hydrolyse CMC and avicel, the cellulase produced by Paenibacillus sp. strain B39 would have potential applications in cellulose biodegradation.

Cultural Conditions Necessary for Optimal Cellulase Yield by Cellulolytic Bacterial Organisms as They Relate to Residual Sugars Released in Broth Medium

“…Due to the promising thermostability and slight acidic tolerance of this enzyme, it has good potential for industrial use in the hydrolysis of soluble cellulose as well as activity on microcrystalline sources of cellulose (Wang et al, 2008). Paenibacillus campinasensis BLII is a thermophilic spore-forming bacterium which was found to grow between 25 and 60 0 C over a wide range of pH.…”

A cold-active acidophilic endoglucanase of Paenibacillus sp. Y2 isolated from soil in an alpine region

“…ion, mostly in Paenibacillus species (Wang et al 2008;Park et al 2012;Dhar et al 2015). The change for the enzyme thermostability by Ca 2+ was not observed, and those by Na…”

“…Because of their merits, such as, high growth rates, greater complexity, multi-enzyme complexes, and their abilities to thrive in different environments, bacteria have been widely exploited for the isolation, purification, and characterization of the novel enzymes (Maki et al 2009). Cellulolytic bacteria, especially Bacillus and Paenibacillus strains, have been isolated from various environment, such as, insect gut (Anand et al 2010;Dantur et al 2015), agricultural environments (Choe et al 2008), soils and composts (Lee et al 1999;Pason et al 2006;Wang et al 2008;Amore et al 2013;Kim et al 2016), feces (Dong et al 2016), and forest soils (Liang et al 2014;Kanchanadumkerng et al 2017).…”

Characterization of Some Efficient Cellulase Producing Bacteria Isolated from Pulp and Paper Mill Effluent Contaminated Soil

“…Microorganisms, which live in soil, are accountable for recycling of this organic carbon to the Environment (14).Degradation of cellulosic materials is a complex process and requires participation of microbial cellulolytic enzymes. Habitats where these substrates are present are the best sources for isolation of cellulolytic microorganisms (15).Several microorganisms have been discovered for decades which have capacity to convert cellulose in to simple sugars (16) but need for newly isolated cellulose degrading microorganisms still continues (17).…”