Characterization of a novel type of adherens junction in meningiomas and the derived cell line HBL-52

Akat, Kemal M.; Bleck, Christopher K. E.; Lee, Young-Min Anna; Haselmann-Weiß, Uta; Kartenbeck, Jürgen

doi:10.1007/s00441-007-0512-5

Cited by 27 publications

(23 citation statements)

References 40 publications

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“…N-cadherin expression in tumor cells is regarded as a sign of invasive behavior. 1,9,12,27 Like E-cadherin, the N-cadherin molecule generally interacts with other N-cadherin molecules expressed in nearby cells in a homophilic manner. 26 Consequently, N-cadherin expressed on a tumor cell may increase its affinity for stromal cells, such as fibroblasts that normally express N-cadherin.…”

Section: Discussionmentioning

confidence: 99%

“…In tumors, invasive behaviors are a sign of progression. 1,9,12,27 Therefore, the mechanism by which canine meningiomas invade into the surrounding nervous tissue might be related to the expression of DCX and N-cadherin. The present study revealed the expression of a series of cell adhesion and related molecules in canine meningiomas by , moderate outliers; *, extreme outliers.…”

Section: Adhesionmentioning

confidence: 99%

“…18 Epithelial cadherin (E-cadherin) is expressed in normal arachnoidal tissue as well as in epithelial tissue and is the main cadherin protein expressed in human meningioma cells. 1,21 The E-cadherin cell adhesion system includes b-catenin, which indirectly connects cadherin to actin filaments. 2,15,25 Altered expression of b-catenin occurs in several human tumors, including gastric, colon, hepatocellular, and esophageal tumors.…”

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confidence: 99%

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Expression of Cell Adhesion Molecules and Doublecortin in Canine Anaplastic Meningiomas

Uchida

Suzuki

et al. 2010

Vet Pathol

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Tumor cell invasion into the surrounding nervous tissue is one of the histologic hallmarks of anaplastic meningiomas. To identify other possible markers for aggression in canine meningiomas, the relationship between histologic features and the expression of molecules involved in cell adhesion, cell proliferation, and invasion was examined. Immunohistochemistry for epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), b-catenin, doublecortin (DCX), and Ki-67 was performed for 55 cases of canine meningioma. DCX was preferentially expressed in tumor cells invading the brain parenchyma (12 of 14 cases), suggesting its involvement in the invasion process. Regardless of the histologic type, E-cadherin and N-cadherin expression was observed in 31 of 55 and 44 of 55 cases, respectively. There was a significant positive correlation between DCX and N-cadherin expression and a significant negative correlation between E-cadherin and N-cadherin expression, suggesting that decreased E-cadherin and increased N-cadherin expression induce DCX expression. Typical membranous b-catenin expression was observed in 10 of 55 cases, whereas nuclear translocation was observed in 33 cases. Nuclear b-catenin expression was frequently found in anaplastic meningiomas (12 of 14 cases). The Ki-67 labeling indices were significantly higher in anaplastic meningiomas than in other types. These findings indicate that the expression of N-cadherin and DCX and the nuclear translocation of b-catenin are closely associated with the presence of invasion and anaplasia in canine meningiomas. Notably, granular cell meningiomas were negative for almost all the molecules examined, suggesting that they have a different tumor biology than other meningiomas.

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Section: Discussionmentioning

confidence: 99%

Section: Adhesionmentioning

confidence: 99%

mentioning

confidence: 99%

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Expression of Cell Adhesion Molecules and Doublecortin in Canine Anaplastic Meningiomas

Uchida

Suzuki

et al. 2010

Vet Pathol

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“…The arachnoid and the pia mater are lined with specialized epithelial cells called meningothelial cells. They are able to establish cell-cell junctions building a firm layer restricting the passage of cells or substances between the CSF and brain tissues or CSF and blood (2)(3)(4). Thus, meningothelial cells, along with endothelial cells of the blood-brain barrier, participate in isolating the central nervous system (CNS) from the rest of the body.…”

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confidence: 99%

Deciphering the Contribution of Human Meningothelial Cells to the Inflammatory and Antimicrobial Response at the Meninges

et al. 2013

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We have investigated the response of primary human meningothelial cells to Neisseria meningitidis. Through a transcriptome analysis, we provide a comprehensive examination of the response of meningothelial cells to bacterial infection. A wide range of chemokines are elicited which act to attract and activate the main players of innate and adaptive immunity. We showed that meningothelial cells expressed a high level of Toll-like receptor 4 (TLR4), and, using a gene silencing strategy, we demonstrated the contribution of this pathogen recognition receptor in meningothelial cell activation. Secretion of interleukin-6 (IL-6), CXCL10, and CCL5 was almost exclusively TLR4 dependent and relied on MyD88 and TRIF adaptor cooperation. In contrast, IL-8 induction was independent of the presence of TLR4, MyD88, and TRIF. Transcription factors NF-B p65, p38 mitogen-activated protein kinase (MAPK), Jun N-terminal protein kinase (JNK1), IRF3, and IRF7 were activated after contact with bacteria. Interestingly, the protein kinase IRAK4 was found to play a minor role in the meningothelial cell response to Neisseria infection. Our work highlights the role of meningothelial cells in the development of an immune response and inflammation in the central nervous system (CNS) in response to meningococcal infection. It also sheds light on the complexity of intracellular signaling after TLR triggering.

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“…It is expressed in normal arachnoid tissue, epithelial tissue and in human meningioma cells. The cytoplasmic tail of the E-cadherin molecules bind to -catenin to form the cadherin -actin filament complex, which is necessary for functional cell to cell adhesion [8,9]. Suppression of expression or cleavage of this adhesion molecule may result in cells acquiring a mesenchymal morphology and increased motility [10].…”

Section: Introductionmentioning

confidence: 99%

Cell Proliferation and Epithelia Profile in Prostate Cancer and Benign Prostatic Hyperplasia

O.M.¹,

P.T.²,

D.T.³

et al. 2014

TOCJ

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Abstract:Objectives: Prostate cancer (PCa) and Benign Prostatic Hyperplasia (BPH) are the leading cause of urinary tract disorders in males both of which are characterized by rapid proliferation of cells. This study characterizes both Ki-67 and E-Cadherin profiles in BPH and PCa biopsies to determine and compare the extent of aggressiveness of cell proliferation in BPH and PCa.Method: Biopsies were collected from patients clinically diagnosed to be suffering from PCa or BPH and were immunohistochemically labelled using Anti-Ki-67 and Anti-ECAD monoclonal antibodies. The sections were treated with urea followed by incubation in a microwave to activate the antigens. Primary antibody treatment was done in biotinylated goat serum, blocking was done with bovine serum albumin (BSA) and finally secondary antibody staining using Anti-Ki-67 and Anti-ECAD monoclonal antibodies. Results:The expression level of Ki-67 corresponded to the rate of proliferation and size of the tumor cell mass in both PCa and BPH. ECAD expression was highly positive in BPH and less positive in PCa-showing onset of malignancy in PCa. Certain cell clumps (metastases) stained highly positive to Ki-67 and were located at random intervals within the tissue of PCa biopsies, this type of distribution also coincided with ECAD immunonegativity. Conclusion:Ki-67 expression indicates the rate of cell proliferation and the aggressiveness of such tumors, while ECAD shows defective cytoskeleton and extent of malignancy. Correlating ECAD and Ki-67 gives a direct relationship between the rate of cell division, tumor invasion and epithelia structure. This also covers both glandular and fibromuscular epithelium

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Characterization of a novel type of adherens junction in meningiomas and the derived cell line HBL-52

Cited by 27 publications

References 40 publications

Expression of Cell Adhesion Molecules and Doublecortin in Canine Anaplastic Meningiomas

Expression of Cell Adhesion Molecules and Doublecortin in Canine Anaplastic Meningiomas

Deciphering the Contribution of Human Meningothelial Cells to the Inflammatory and Antimicrobial Response at the Meninges

Cell Proliferation and Epithelia Profile in Prostate Cancer and Benign Prostatic Hyperplasia

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