Characterization of a Novel Zinc Finger Gene (ZNF165) Mapping to 6p21 That Is Expressed Specifically in Testis

Tirosvoutis, Konstantinos N.; Divane, Aspasia; Jones, Michael H.; Affara, Nabeel A.

doi:10.1006/geno.1995.1178

Cited by 27 publications

(17 citation statements)

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“…ZNF165 mRNA detected in tumours is 2.2 kb, consistent with that found in the testis (Tirosvoutis et al , 1995). The ORF of ZNF165 cDNA encodes a predicted protein of 485 amino-acid residues.…”

Section: Discussionsupporting

confidence: 86%

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Zinc-finger protein ZNF165 is a novel cancer-testis antigen capable of eliciting antibody response in hepatocellular carcinoma patients

et al. 2004

Br J Cancer

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ZNF165 is a zinc-finger protein gene that was identified from human adult testis. Analysis of the origins of publicly available expressed sequence tags as presented in Unigene and SAGE databases revealed that ZNF165 mRNA was expressed in tumours of different tissues. RT–PCR, real-time PCR and Northern blotting analysis confirmed that ZNF165 mRNA was expressed in the hepatocellular carcinoma, gastric cancer, colon cancer and non-small-cell lung carcinoma. The nucleotide sequence of ZNF165 expressed in tumours is identical to that expressed in the testis. Humoral responses of hepatocellular carcinoma (HCC) patients against ZNF165 protein were determined by Western blotting using the recombinant ZNF165 protein. Antibodies against ZNF165 protein were detected in approximately 5% (four of 82) of the sera from HCC patients. These results suggest that ZNF165, a member of the ZNF family, is a novel CT antigen capable of eliciting humoral immune response and be involved in tumour biology.

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Section: Discussionsupporting

confidence: 86%

“…ZNF165, expressed in human adult testis, is a member of kruppel family of zinc-finger-containing transcription factors (Tirosvoutis et al , 1995). In this report, we have examined ZNF165 mRNA expression in tumours of different histological types using various approaches.…”

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confidence: 99%

Zinc-finger protein ZNF165 is a novel cancer-testis antigen capable of eliciting antibody response in hepatocellular carcinoma patients

et al. 2004

Br J Cancer

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“…In addition, the expression of HLA antigens during spermatogenesis has been documented (Martin-Villa et al 1996). Some of the human homolog of murine T/t complex genes, which are expressed in testis, are also close to the human MHC complex (Ragoussis et al 1992;Tirosvoutis et al 1995). Therefore, the aim of the present study has been to investigate whether HLA class I or II is associated with nonobstructive azoospermia, and whether a gene responsible for this disorder exists in the HLA region.…”

Section: Discussionmentioning

confidence: 93%

“…A prominent characteristic of the HLA genes is a marked degree of polymorphism, and the HLA antigens are considered important factors in the susceptibility to some disorders. MHC-associated effects on fertility as described in mice have also been suggested for humans (Gill 1994), because the spermatogenic genes have been identified within or close to the human HLA class II region in analogy to the murine T/t complex (Ragoussis et al 1992;Tirosvoutis et al 1995). Significant differences of the HLA class II allele and haplotype frequencies between males with normal spermatogenesis and males with infertility have been reported (van der Ven et al 2000).…”

Section: Introductionmentioning

confidence: 89%

Susceptibility gene for non-obstructive azoospermia located near HLA-DR and -DQ loci in the HLA class II region

et al. 2002

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The technical developments and expanded indications for testicular sperm extraction (TESE) with intracytoplasmic sperm injection (ICSI) provide great advantages for patients with non-obstructive azoospermia. Such success, however, also means that genetic abnormalities in non-obstructive azoospermia can be transmitted to the next generation, demonstrating the importance of being able to understand the genetic background of non-obstructive azoospermia. We have previously reported that human leukocyte antigens (HLA)-A33 and -B44 in the HLA class I region and the HLA-DRB1*1302 allele in the HLA class II region are linked to susceptibility to non-obstructive azoospermia in Japanese men. However, strong linkage of HLA-DRB1*1302 with HLA-A33 and -B44 is also evident in the Japanese population. Thus, uncertainty prevails as to whether the HLA class I or class II molecule is more directly associated with non-obstructive azoospermia. In the present study, we performed association analysis with 21 polymorphic microsatellite markers identified near the HLA genes to map the gene involved in the development of non-obstructive azoospermia more precisely. Microsatellite markers located in the HLA class I region or the class III region showed no statistically significant association with this disorder, although once again the HLA-A33 and -B44 alleles showed a significant association. In contrast, some of the microsatellite markers in the HLA class II region and at the HLA-DRB1 and -DQB1 loci displayed strong associations with non-obstructive azoospermia. Taken together, our previous and present data suggest that the critical region for development of non-obstructive azoospermia is near the HLA-DRB1 and -DQB1 segments in the HLA class II region.

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“…29,38 Three of these, ZNF184, ZNF165, and LD5 have been shown to be predominantly expressed in the testis 7,38,39 and two, F20 and F36, have strong homology with the murine ZNF genes, Zfp-38, Zfp-92, and Zfp-35, known to have a role in spermatogenesis. [40][41][42] The location and expression of the ZNF genes identified here correlate well with those from analyses of the syntenic region of the mouse which have shown the presence of a cluster of ZNF genes involved in embryogenesis and spermatogenesis.…”

Section: Znf Gene Clustermentioning

confidence: 99%

Generation of a transcription map distal to HLA-F

et al. 1998

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We have constructed a transcription map covering a 2 Mb region beginning approximately 1 Mb distal to HLA-F. Cosmids isolated from a chromosome 6 library were positioned by YAC hybridisation, STS and fingerprint analysis. Using direct cDNA selection, exon trapping, and direct genomic sequence analysis, we identified 42 potential exonic fragments in this region. Six fragments corresponded to previously characterised genes, four previously broadly mapped to this region. Five fragments were similar to known genes, eight fragments matched ESTs and 10 of the remaining 23 novel fragments, gave a positive signal on northern analysis. All cDNA fragments were mapped to the YAC and cosmid contig covering the region and with respect to other known genes and STS in this area. The distribution of the cDNA fragments indicated their organisation in three clusters around CpG islands.

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Characterization of a Novel Zinc Finger Gene (ZNF165) Mapping to 6p21 That Is Expressed Specifically in Testis

Cited by 27 publications

References 0 publications

Zinc-finger protein ZNF165 is a novel cancer-testis antigen capable of eliciting antibody response in hepatocellular carcinoma patients

Zinc-finger protein ZNF165 is a novel cancer-testis antigen capable of eliciting antibody response in hepatocellular carcinoma patients

Susceptibility gene for non-obstructive azoospermia located near HLA-DR and -DQ loci in the HLA class II region

Generation of a transcription map distal to HLA-F

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