Characterization of a Papovavirus Isolated from Fledgling Budgerigars

Lh, Bozeman; Rb, Davis; D, Gaudry; Pd, Lukert; Oj, Fletcher; Mj, Dykstra

doi:10.2307/1590072

Cited by 86 publications

(37 citation statements)

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“…These changes are consistent with the changes described in APV infected birds previously (Bernier et al, 1981;Bozeman et al, 1981;Davis et al, 1981;Graham and Calnek, 1987). Changes seen in the male such as proliferation of lymphocytes, gliosis, and neuronal degeneration are more typical of the paramyxovirus infection.…”

Section: Discussionsupporting

confidence: 89%

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Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction

Tomášek¹,

Kubicek²,

Tukač³

2007

Vet. Med. - Czech

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High mortality of nestling cockatiels (Nymphicus hollandicus) was observed in one breeding flock in Slovakia. The nestling mortality affected 50% of all breeding pairs. In general, all the nestlings in affected nests died. Death occurred suddenly in 4-to 6-day-old birds, most of which had full crops. No feather disorders were diagnosed in this flock. Two dead nestlings were tested by nested PCR for the presence of avian polyomavirus (APV) and Chlamydophila psittaci and by single-round PCR for the presence of beak and feather disease virus (BFDV). After the breeding season ended, a breeding pair of cockatiels together with their young one and a fledgling budgerigar (Melopsittacus undulatus) were examined. No clinical alterations were observed in these birds. Haemorrhages in the proventriculus and irregular foci of yellow liver discoloration were found during necropsy in the young cockatiel and the fledgling budgerigar. Microscopy revealed liver necroses and acute haemolysis in the young cockatiel and confluent liver necroses and heart and kidney haemorrhages in the budgerigar. Two dead cockatiel nestlings, the young cockatiel and the fledgling budgerigar were tested positive for APV, while the cockatiel adults were negative. The presence of BFDV or Chlamydophila psittaci DNA was detected in none of the birds. The specificity of PCR was confirmed by the sequencing of PCR products amplified from the samples from the young cockatiel and the fledgling budgerigar. The sequences showed 99.6-100% homology with the previously reported sequences. To our knowledge, this is the first report of APV infection which caused a fatal disease in parent-raised cockatiel nestlings and merely subclinical infection in budgerigar nestlings.

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Section: Discussionsupporting

confidence: 89%

“…APV is an uncoated virus 40-48 nm in diameter (Bernier et al, 1981;Bozeman et al, 1981;Davis et al, 1981). Its genome consists of the circular double-stranded DNA (Lehn and Muller, 1986;Rott et al, 1988).…”

mentioning

confidence: 99%

Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction

Tomášek¹,

Kubicek²,

Tukač³

2007

Vet. Med. - Czech

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“…The LM and TEM appearance of these polyomavirus inclusions was identical to the appearance of avian polyomavirus inclusions described in psittaciformes by others (Bozeman et al, 1981;Muller & Nitschke, 1986;Larimer et al, 1993;Garcia et al, 1994a, b). Our DNA hybridization results suggest that the small nuclear granules found during TEM examination might have consisted of polyomavirus proteins (incomplete virus).…”

Section: Discussionsupporting

confidence: 84%

“…Although polyomavirus is a feature of lesions and mortality in fledgling budgerigars infected with budgerigar fledgling disease virus (Bozeman et al, 1981;Muller & Nitschke, 1986), we believe that polyomavirus infection in the present study was an incidental finding. We attributed the diarrhoea in these hens to intestinal parasitism.…”

Section: Discussionmentioning

confidence: 55%

Polyomavirus inclusion bodies in chicken caecal epithelium

Goodwin

Lattmer

Player³

et al. 1996

Avian Pathology

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“…Development of this inactivated vaccine was achieved because the virus was cultivable in chicken embryo fibroblast cells (Bozeman et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Recombinant subunit vaccine elicits protection against goose haemorrhagic nephritis and enteritis

Mató¹,

Pénzes²,

Rueda³

et al. 2009

Avian Pathology

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Outbreaks of haemorrhagic nephritis and enteritis of geese (HNEG) have been reported in goose flocks in Hungary, Germany and France since 1969. HNEG is characterized by high morbidity and mortality rates in geese 3 to 10 weeks of age. The causative agent of HNEG is the goose haemorrhagic polyomavirus (GHPV), which has a circular double-stranded DNA genome encoding the structural proteins VP1, VP2 and VP3. In vitro culture of GHPV has been problematic, so the baculovirus system was used to construct a recombinant virus expressing the VP1 gene of GHPV under control of the polyhedrin promoter in Sf9 insect cells. The expression and the identity of recombinant goose polyomavirus VP1 in the crude Sf9 cell extracts were confirmed by mass spectrometry. Experimental oilÁemulsion vaccines containing two different doses of antigen were prepared using this crude extract. Goslings were vaccinated either once at 1 day old or twice by boosting 18 days after the primary vaccination, and were challenged with a virulent polyomavirus isolate at 5 weeks of age. A single injection of either vaccine dose induced 95% protection against challenge. Using the booster vaccination regimen, 100% protection was achieved with either vaccine dose.

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Characterization of a Papovavirus Isolated from Fledgling Budgerigars

Cited by 86 publications

References 1 publication

Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction

Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction

Polyomavirus inclusion bodies in chicken caecal epithelium

Recombinant subunit vaccine elicits protection against goose haemorrhagic nephritis and enteritis

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