Characterization of a Pathogen Induced Thaumatin-Like Protein Gene AdTLP from Arachis diogoi, a Wild Peanut

Abstract

Peanut (Arachis hypogaea L) is one of the widely cultivated and leading oilseed crops of the world and its yields are greatly affected by various biotic and abiotic stresses. Arachis diogoi, a wild relative of peanut, is an important source of genes for resistance against various stresses that affect peanut. In our previous study a thaumatin-like protein gene was found to be upregulated in a differential expression reverse transcription PCR (DDRT-PCR) study using the conidial spray of the late leaf spot pathogen, Phaeoisariopsis personata. In the present study, the corresponding full length cDNA was cloned using RACE-PCR and has been designated as AdTLP. It carried an open reading frame of 726 bp potentially capable of encoding a polypeptide of 241 amino acids with 16 conserved cysteine residues. The semi-quantitative RT-PCR analysis showed that the transcript level of AdTLP increased upon treatment with the late leaf spot pathogen of peanut, P. personata and various hormone treatments indicating its involvement in both, biotic and abiotic stresses. The antifungal activity of the purified recombinant protein was checked against different fungal pathogens, which showed enhanced anti-fungal activity compared to many other reported TLP proteins. The recombinant AdTLP-GFP fusion protein was found to be predominantly localized to extracellular spaces. Transgenic tobacco plants ectopically expressing AdTLP showed enhanced resistance to fungal pathogen, Rhizoctonia solani. The seedling assays showed enhanced tolerance of AdTLP transgenic plants against salt and oxidative stress. The transcript analysis of various defense related genes highlighted constitutively higher level expression of PR1a, PI-I and PI-II genes in transgenic plants. These results suggest that the AdTLP is a good candidate gene for enhancing stress resistance in crop plants.

Characterization of Withania somnifera Leaf Transcriptome and Expression Analysis of Pathogenesis – Related Genes during Salicylic Acid Signaling

“…Concurrently, the up-regulation of thaumatin-like PR-5 during SA treatment was reported from Malus hupehensis [22], sorghum [130], wheat [155], Arachis diogoi [156] and Eucalyptus grandis [135].…”

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

“…TLP have well described antifungal activity, causing osmotic breakage of transmembrane pores of the fungal plasma membranes [43]. Transgenic plants constitutively overexpressing TLPs often show enhanced fungal [44–48] and bacteria resistance [49]. Methyltransferase type 11 was one of the five most highly-expressed genes among the DEGs.…”

Agrobacterium-Mediated Transformation of the Oryza sativa Thaumatin-Like Protein to Canola (R Line Hyola308) for Enhancing Resistance to Sclerotinia sclerotiorum

“…In agreement with our fi nding, Zamani et al (24) have reported that transgenic canola harboring rye tlp gene could inhibit the progress of the S. sclerotiorum fungal growth (24). The same fi ndings have also reported that overexpression of tlp gene demonstrates an inhibitory eff ect on phytopathogenic fungi through inhibition of the spore germination and mycelial growth (11,25,26).…”