Characterization of a pathogenesis-related thaumatin-like protein gene<i>TaPR5</i>from wheat induced by stripe rust fungus

Wang, Xiaojie; Tang, Chunlei; Deng, Lin; Gaolei, Cai; Liu, Xinying; Liu, Bo; Han, Qingmei; Buchenauer, H.; Wei, Guorong; Han, Dejun; Huang, Lili; Kang, Zhensheng

doi:10.1111/j.1399-3054.2009.01338.x

Cited by 83 publications

(63 citation statements)

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“…lini infection in flax; these genes are commonly regulated by plants in pathogenic attacks (Kirubakaran et al, 2008; Wang et al, 2010; Kawahara et al, 2012; Li et al, 2013; Xiao et al, 2013; Lowe et al, 2014; Teixeira et al, 2014; Curto et al, 2015). Thaumatins (PR-5) can cause increased permeabilization of the fungal cell wall inflicting direct damage to fungal hyphae (Vigers, 1991; Vigers et al, 1992).…”

Section: Resultsmentioning

confidence: 99%

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

Galindo‐González

Deyholos

2016

Front. Plant Sci.

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Fusarium oxysporum f. sp. lini is a hemibiotrophic fungus that causes wilt in flax. Along with rust, fusarium wilt has become an important factor in flax production worldwide. Resistant flax cultivars have been used to manage the disease, but the resistance varies, depending on the interactions between specific cultivars and isolates of the pathogen. This interaction has a strong molecular basis, but no genomic information is available on how the plant responds to attempted infection, to inform breeding programs on potential candidate genes to evaluate or improve resistance across cultivars. In the current study, disease progression in two flax cultivars [Crop Development Center (CDC) Bethune and Lutea], showed earlier disease symptoms and higher susceptibility in the later cultivar. Chitinase gene expression was also divergent and demonstrated and earlier molecular response in Lutea. The most resistant cultivar (CDC Bethune) was used for a full RNA-seq transcriptome study through a time course at 2, 4, 8, and 18 days post-inoculation (DPI). While over 100 genes were significantly differentially expressed at both 4 and 8 DPI, the broadest deployment of plant defense responses was evident at 18 DPI with transcripts of more than 1,000 genes responding to the treatment. These genes evidenced a reception and transduction of pathogen signals, a large transcriptional reprogramming, induction of hormone signaling, activation of pathogenesis-related genes, and changes in secondary metabolism. Among these, several key genes that consistently appear in studies of plant-pathogen interactions, had increased transcript abundance in our study, and constitute suitable candidates for resistance breeding programs. These included: an induced RPMI-induced protein kinase; transcription factors WRKY3, WRKY70, WRKY75, MYB113, and MYB108; the ethylene response factors ERF1 and ERF14; two genes involved in auxin/glucosinolate precursor synthesis (CYP79B2 and CYP79B3); the flavonoid-related enzymes chalcone synthase, dihydroflavonol reductase and multiple anthocyanidin synthases; and a peroxidase implicated in lignin formation (PRX52). Additionally, regulation of some genes indicated potential pathogen manipulation to facilitate infection; these included four disease resistance proteins that were repressed, indole acetic acid amido/amino hydrolases which were upregulated, activated expansins and glucanases, amino acid transporters and aquaporins, and finally, repression of major latex proteins.

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Section: Resultsmentioning

confidence: 99%

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

Galindo‐González

Deyholos

2016

Front. Plant Sci.

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“…Pathogenesis-related (PR) thaumatin-like proteins are induced and accumulate locally and often systemically in plant tissues in response to infection and various abiotic and biotic stresses (Hammerschmidt and Nicholson 1999). Beside stress stimuli, proteins of the PR5 family, also named thaumatin-like proteins, are induced by phytohormones (methyl jasmonate, salicylic acid, and abscisic acid) (Wang et al 2010). …”

Section: Stress and Defense-related Proteinsmentioning

confidence: 99%

Proteomic signature of fenugreek treated by methyl jasmonate and cholesterol

et al. 2017

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Main conclusion Changes in proteome level as a result of methyl jasmonate and cholesterol treatment were investigated. The identified proteins were often involved in response to stress caused by various treatments. Furthermore, 18 proteins were expressed in treatmentspecific manner. In this study, the fenugreek plants were treated with methyl jasmonate (as an elicitor) and cholesterol (as a precursor of steroids and steroidal saponins) to check reaction at the level of the proteome to stress and to investigate steroidal saponin (diosgenin) biosynthesis. Proteins were separated by two-dimensional electrophoresis (2-DE) and identified by MALDI-ToF/ToF followed by database searches using Mascot search engine. Totally, 63 and 41 protein spots were differentially expressed after methyl jasmonate and cholesterol treatment, respectively. These proteins were classified into seven groups: photosynthesis, energy, metabolism, protein metabolism, secondary metabolism, stress and defense, and other. We found that 9 proteins were responsive to all treatments, and 18 proteins expressed in treatment-specific manner. Higher level of photosynthetic proteins sensitive to both biotic and abiotic stimuli was detected. In addition, proteins related to the stress (especially oxidative) and defense, protein, and secondary metabolism were overexpressed. The results indicate that methyl jasmonate and cholesterol elicited a defense reaction at the proteome level as a response to stress. The usefulness of 2-DE method for identification of proteins related with species-specific metabolic pathways is restricted. Integration of transcriptome data with proteomic analysis improved annotation process.

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“…Thaumatin like protein [33] and β-1,3-glucanase [34] were upregulated in both compatible and incompatible wheat-Pst interactions, during which they may participate in the basal defense of wheat against Pst. The higher level of transcription and greater abundance of protein labeling in the incompatible wheat-Pst interaction than in the compatible interaction further suggested their positive role in wheat resistance to Pst [33,34] . Combined with the induced expression in response to phytohormones (SA, jasmonic acid and abscisic acid), it is reasonable to suggest that these two PR proteins are involved in both SAR and induced systemic resistance.…”

Section: Pathogenesis-related Proteinsmentioning

confidence: 99%

New insights in the battle between wheat and Puccinia striiformis

Tang¹,

Wang²,

Cheng³

et al. 2015

Front. Agr. Sci. Eng.

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Wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) poses a great threat to wheat production worldwide. The rapid change in virulence of Pst leads to a loss of resistance in currently resistant wheat cultivars, which results in frequent disease epidemics. Therefore, a major focus is currently placed on investigating the molecular mechanisms underlying this rapid variation of pathogenicity and coevolving wheat resistance. Limited by the lack of a system for stable transformation of Pst and the difficulties in wheat transformation, it is not easy to generate deeper insights into the wheat-Pst interaction using established genetic methods. Nevertheless, considerable effort has been made to unravel the wheat-Pst interaction and significant progress is being made. Histology and cytology have revealed basic details of infection strategies and defense responses during wheat-Pst interactions, identified cellular components involved in wheat-Pst interactions, and have helped to elucidate their role in the infection process or in plant defense responses. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat-Pst pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance response and in Pst virulence. Studies of wheat-Pst interactions have now entered a new phase in which cellular and molecular approaches are being used. This review focuses on the cellular biology of wheat-Pst interactions and integrates the emerging data from molecular analyses with the histocytological observations.

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Characterization of a pathogenesis-related thaumatin-like protein geneTaPR5from wheat induced by stripe rust fungus

Cited by 83 publications

References 50 publications

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

RNA-seq Transcriptome Response of Flax (Linum usitatissimum L.) to the Pathogenic Fungus Fusarium oxysporum f. sp. lini

Proteomic signature of fenugreek treated by methyl jasmonate and cholesterol

New insights in the battle between wheat and Puccinia striiformis

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