Characterization of a polysaccharide from Eupolyphaga sinensis walker and its effective antitumor activity via lymphocyte activation

Xie, Xin; Shen, Wen Hong; Zhou, Yiran; Ma, Lingman; Xu, Duiyue; Ding, Jinfeng; He, Liangyuan; Shen, Baiyong; Zhou, Changlin

doi:10.1016/j.ijbiomac.2020.06.120

Cited by 31 publications

(27 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study found that CPP promotes spleen lymphocyte proliferation and inhibits the regulatory effect of LPS on lymphocyte proliferation. Besides, the polysaccharides from Eupolyphaga Sinensis Walker and sulfated Chinese yam polysaccharides could also promote splenic lymphocyte proliferation [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

The Immunomodulatory Effects of Phellodendri Cortex Polysaccharides on Cyclophosphamide-Induced Immunosuppression in Mice

Cheng

Liu

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Cyclophosphamide is a commonly used anticancer drug, and immunosuppression is one of the most common side effects. How to recover the immunological function is important for cyclophosphamide-treated patients. In the present study, Phellodendri Cortex polysaccharides (CPP) could enhance the proliferation of mouse spleen lymphocytes in vitro. The immunoregulatory function of CPP was then investigated in cyclophosphamide-induced immunosuppressed mice. In CPP-treated groups, mice were orally treated with CPP at doses of 1, 0.5, and 0.25 g/kg bodyweight from 1 to 11 d, respectively. The cyclophosphamide was administrated in CPP and cyclophosphamide groups from 12 to 14 d. In the cyclophosphamide and normal control groups, the mice received equal volume of saline from 1 to 14 d. The results showed that CPP (1 g/kg) could significantly increase the bodyweight of mice, even during cyclophosphamide treatment. The organ coefficients of the spleen and thymus were recovered by CPP treatment. CPP upregulated the contents of cytokines (IL-2, IL-6, IFN-γ, and TNF-α) in serum, which were downregulated by cyclophosphamide. The mRNA levels of these cytokines were also elevated by CPP treatment in the spleen. Cyclophosphamide upregulated the expressions of NF-κB p65, TLR4, and MyD88, suggesting that the NF-κB signaling pathway was activated by cyclophosphamide. After CPP treatment, it was recovered to normal level. These results indicated that CPP alleviated the cyclophosphamide-induced immunosuppression.

show abstract

Section: Discussionmentioning

confidence: 99%

The Immunomodulatory Effects of Phellodendri Cortex Polysaccharides on Cyclophosphamide-Induced Immunosuppression in Mice

Cheng

Liu

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…The hydrolyzed residue was redissolved in 2 ml of chromatographic methanol three times to remove the remaining TFA. Finally, 0.5 ml ultrapure water was added to the sample residue to prepare a polysaccharide hydrolysate (Xie et al, 2020).…”

Section: Acid Hydrolysis Of Samplementioning

confidence: 99%

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

Qiao

Cai

et al. 2021

J. Food Process. Preserv.

View full text Add to dashboard Cite

The purpose of the present study is to illustrate the effects of extraction methods on the antioxidant activity and rheological properties of polysaccharides isolated from Ribes stenocarpum Maxim (RPs). RPs were extracted by ultrasonic-assisted extraction (RPs-1), alkaline extraction (RPs-2), acid extraction (RPs-3), microwaveassisted extraction (RPs-4), and three-phase separation (RPs-5). The results showed that RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 are acidic heteropolysaccharides with glucose, arabinose, and galacturonic acid as the main chain units and absence of triple-helix structure. Of them, RPs-3 possess the highest uronic acid content, best thermal stability, and strongest DPPH, ABTS, and hydroxyl radical scavenging abilities. Rheological properties showed that the above-mentioned polysaccharides are non-Newtonian fluids and own the similar shear thinning properties. RPs-3 represent the highest apparent viscosity and better stability in the over-acid or overalkali environment. The above results indicated that the acid extraction could serve as the most favorable extraction method for RPs. Novelty impact statement: A novel polysaccharide was isolated from Ribes stenocarpumMaxim by acid extraction, showing a stronger antioxidant activity and excellent rheological properties, which has a good application prospect in food processing.

show abstract

“…Recently, a new water-soluble polysaccharide, named ESPS, was separated from ESW in our laboratory. The primary chemical structure was studied with an average molecular weight of 21.4 kDa, and containing rhamnose (7.4%), fucose (3.1%), arabinose (13.9%), xylose (9.3%), glucose (39.7%), and galactose (26.5%) ( Xie et al, 2020 ). We applied for a patent on its structure and use; the patent publication number is CN110894244A.…”

Section: Introductionmentioning

confidence: 99%

A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo

Zhang

Ding

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

Hepatitis B virus (HBV) infection remains a major global threat to human health worldwide. Recently, the Chinese medicines with antiviral properties and low toxicity have been a concern. In our previous study, Eupolyphaga sinensis Walker polysaccharide (ESPS) has been isolated and characterized, while its antiviral effect on HBV remained unclear. The anti-HBV activity of ESPS and its regulatory pathway were investigated in vitro and in vivo. The results showed that ESPS significantly inhibited the production of HBsAg, HBeAg, and HBV DNA in the supernatants of HepG2.2.15 in a dose-dependent manner; HBV RNA and core protein expression were also decreased by ESPS. The in vivo studies using HBV transgenic mice further revealed that ESPS (20 and 40 mg/kg/2 days) significantly reduced the levels HBsAg, HBeAg, and HBV DNA in the serum, as well as HBV DNA and HBV RNA in mice liver. In addition, ESPS activated the Toll-like receptor 4 (TLR4) pathway; elevated levels of IFN-β, TNF-α, and IL-6 in the serum were observed, indicating that the anti-HBV effect of ESPS was achieved by potentiating innate immunity function. In conclusion, our study shows that ESPS is a potential anti-HBV ingredient and is of great value in the development of new anti-HBV drugs.

show abstract

Characterization of a polysaccharide from Eupolyphaga sinensis walker and its effective antitumor activity via lymphocyte activation

Cited by 31 publications

References 48 publications

The Immunomodulatory Effects of Phellodendri Cortex Polysaccharides on Cyclophosphamide-Induced Immunosuppression in Mice

The Immunomodulatory Effects of Phellodendri Cortex Polysaccharides on Cyclophosphamide-Induced Immunosuppression in Mice

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo

Contact Info

Product

Resources

About