Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.

Singer, Dinah S.; Camerini‐Otero, R. Daniel; Satz, M. L.; Osborne, Barbara A.; Sachs, David H.; Rudikoff, Stuart

doi:10.1073/pnas.79.5.1403

Cited by 85 publications

(52 citation statements)

References 31 publications

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“…The MHC class I promoter construct used in these studies derived from the swine class I gene PD1 (64,65). The PD1 promoter truncation series, ligated to the cat gene (chloramphenicol acetyl transferase (CAT)) reporter, was previously described (66).…”

Section: Plasmids and Cloning Strategiesmentioning

confidence: 99%

A T Lymphocyte-Specific Transcription Complex Containing RUNX1 Activates MHC Class I Expression

Howcroft

Weissman

Gégonne

et al. 2005

The Journal of Immunology

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MHC class I expression is subject to both tissue-specific and hormonal regulatory mechanisms. Consequently, levels of expression vary widely among tissues, with the highest levels of class I occurring in the lymphoid compartment, in T cells and B cells. Although the high class I expression in B cells is known to involve the B cell enhanceosome, the molecular basis for high constitutive class I expression in T cells has not been explored. T cell-specific genes, such as TCR genes, are regulated by a T cell enhanceosome consisting of RUNX1, CBFβ, LEF1, and Aly. In this report, we demonstrate that MHC class I gene expression is enhanced by the T cell enhanceosome and results from a direct interaction of the RUNX1-containing complex with the class I gene in vivo. T cell enhanceosome activation of class I transcription is synergistic with CIITA-mediated activation and targets response elements distinct from those targeted by CIITA. These findings provide a molecular basis for the high levels of MHC class I in T cells.

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Section: Plasmids and Cloning Strategiesmentioning

confidence: 99%

A T Lymphocyte-Specific Transcription Complex Containing RUNX1 Activates MHC Class I Expression

Howcroft

Weissman

Gégonne

et al. 2005

The Journal of Immunology

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“…Recently, a porcine genomic clone containing a functional gene encoding a class I transplantation (SLA) antigen was isolated (30 Reiteration frequency mapping by the "contact blot" method was as described by Zasloff and Santos (42).…”

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confidence: 99%

“…In mice the intergenic distances range from less than 10 kilobases (kb) to greater 903 on May 9, 2018 by guest http://mcb.asm.org/ Downloaded from than 35 kb (35). In miniature swine, this distance is at least 10 kb (30). The nature of the DNA sequences occurring between MHC genes is unknown.…”

mentioning

confidence: 99%

“…Recently, this analysis has been extended to an assessment of the DNA sequence organization of the MHC, and a variety of genes encoding MHC antigens from humans (20,26,32), mice (7,13,35), and miniature swine (30) have been isolated. It has been estimated that the number of sequences homologous to class I heavy-chain genes ranges from about 12 in swine (30) to approximately 40 in some strains of mice (35). To date, analysis of DNA sequences of MHC genes from both humans (20) and mice (35) reveals the common eucaryotic gene organization: separate exons encode the three external polypeptide domains and the transmembrane and cytoplasmic segments.…”

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confidence: 99%

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Specific association of repetitive DNA sequences with major histocompatibility genes.

Singer¹,

Lifshitz²,

Abelson³

et al. 1983

Mol. Cell. Biol.

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The DNA sequence organization of a 17.8-kilobase segment of porcine DNA, containing a functional major histocompatibility (MHC) The major histocompatibility complex (MHC) in humans and other vertebrates encodes a series of highly polymorphic cell-surface glycoproteins involved in a variety of immunological phenomena (18,29). The transplantation antigens (class I) are of particular interest in that they are targets in, and restricting elements for, cytotoxic lymphocyte reactions (43) and are probably the major antigens involved in graft rejection. These antigens consist of two associated polypeptides, a heavy chain (45,000 daltons) and a light chain identified as b2-microglobulin. Genetic studies have shown that in each species there are a number of distinct loci within the MHC which encode class I heavy chains. Extensive serological (1,17) and biochemical analyses (24) of transplantation antigens, including detailed amino acid sequence determinations (6,25,38), have revealed that the heavy chains are highly polymorphic in humans, mice, and miniature swine. More than 50 different alleles at each locus have been identified in mice (18). Thus, the heavy chains of transplantation antigens represent one of the most polymorphic multigene families known in eucaryotes. The mechanisms generating this diversity are not known. In contrast, b2-microglobulin, which is not encoded in the MHC, is essentially invariant within a species.The genetic complexity, diversity, and biological importance of the MHC have provided the impetus for numerous studies contributing to the characterization of this complex in a variety of species. Recently, this analysis has been extended to an assessment of the DNA sequence organization of the MHC, and a variety of genes encoding MHC antigens from humans (20,26,32), mice (7,13,35), and miniature swine (30) have been isolated. It has been estimated that the number of sequences homologous to class I heavy-chain genes ranges from about 12 in swine (30) to approximately 40 in some strains of mice (35). To date, analysis of DNA sequences of MHC genes from both humans (20) and mice (35) reveals the common eucaryotic gene organization: separate exons encode the three external polypeptide domains and the transmembrane and cytoplasmic segments. Studies on the structure of the MHC class I genes have led to the proposal (13) that the extensive polymorphism observed in the MHC may in part be generated by gene conversions in addition to more well defined mechanisms, such as point mutations and recombination. However, it is apparent that analyses of structural genes alone will not totally elucidate the regulatory events leading to differential expression of MHC genes and the generation of polymorphism, nor will they reveal the evolutionary processes occurring within the MHC.Another approach is to examine the organization and structure of DNA sequences linked to MHC genes. In mice the intergenic distances range from less than 10 kilobases (kb) to greater 903 on May 9, 2018 by guest

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“…2) [33]. All Ia genes code for a polymorphic heavy glycoprotein chain of about 45 kiloDaltons (kDa) which binds non-covalently to the monomorphic β2-microglobulin at the cell membrane [35]. The polymorphism of swine SLA class Ia genes corresponds to amino acid substitutions in positions involved either in fixing the foreign peptides or in establishing direct contact with the T -cell-receptor [8].…”

Section: The Sla Ia Genesmentioning

confidence: 99%

The porcine Major Histocompatibility Complex and related paralogous regions: a review

Chardon¹,

Renard²,

Gaillard³

et al. 2000

Genet. Sel. Evol.

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-The physical alignment of the entire region of the pig major histocompatibility complex (MHC) has been almost completed. In swine, the MHC is called the SLA (swine leukocyte antigen) and most of its class I region has been sequenced. Over one hundred genes have been characterised, including the classical class I and class I-related genes, as well as the class II gene families. These results in swine provide new evidence for the striking conservation during the evolution of a general MHC framework, and are consistent with the location of the class I genes on segments referred to as permissive places within the MHC class I region. Recent results confirm the involvement of the SLA region in numerous quantitative traits.

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Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.

Cited by 85 publications

References 31 publications

A T Lymphocyte-Specific Transcription Complex Containing RUNX1 Activates MHC Class I Expression

A T Lymphocyte-Specific Transcription Complex Containing RUNX1 Activates MHC Class I Expression

Specific association of repetitive DNA sequences with major histocompatibility genes.

The porcine Major Histocompatibility Complex and related paralogous regions: a review

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