Characterization of a potentially novel ‘blown pack’ spoilage bacterium isolated from bovine hide

Abstract

Aims: To characterize a psychrotrophic bacterium, designated TC1, previously isolated from a cattle hide in Ireland, and to investigate the ability of this strain to cause 'blown pack' spoilage (BPS) of vacuum-packaged beef primals. Methods and Results: TC1 was characterized using a combination of phenotypic, chemotaxonomic and genotypic analyses and was assessed for its ability to spoil vacuum-packaged beef at refrigerated temperatures. TC1 was Gram-positive and formed elliptical subterminal endospores. The strain was able to grow between 0 and 33°C, with optimal growth between 23 and 24°C. TC1 could be differentiated from its phylogenetically closest neighbour (Clostridium lituseburense DSM 797 T ) by 16S rRNA gene sequencing, pulsedfield gel electrophoresis and cellular fatty acid composition. TC1 spoiled (BPS) beef within 42 days when inoculated in cold-stored (1°C) vacuum-packed beef. Conclusions: The phenotypic, chemotaxonomic and genotypic characterization indicated that TC1 may represent a potentially novel, cold-tolerant, gasproducing bacterium of considerable economic significance to the beef industry. Significance and Impact of the Study: This study reports and characterizes an emerging BPS bacterium, which should be considered in future activities designed to minimize the psychrophilic and psychrotrophic spoilage of vacuum-packaged beef.

Comparative Genome Analysis and Phenotypic Characterization of Clostridium gasigenes CGAS001 Isolated From Chilled Vacuum-Packed Lamb Meat

“…Ten spores of C. gasigenes seem to be enough to cause BPS in vacuum packed meat (Moschonas et al, 2010), with the BPS occurring within 36 days or 56 days at 1 • C depending on applied meat processing techniques (Moschonas et al, 2011b). The spores are therefore a primary target for reducing the occurrence of BPS (Moschonas and Bolton, 2013). Data have shown, that oxygen exposure is a possible option to prevent growth of germinated C. gasigenes spores (Adam et al, 2013).…”

A comparative and functional genomics analysis of the genusRomboutsiaprovides insight into adaptation to an intestinal lifestyle

“…The isolation of strains from intestinal sources that possibly belong to other novel species within the genus Romboutsia has been reported as well. For example, strain TC1 was isolated from the hide of a cow, where it was found likely as the result of a faecal contamination of the hide (14). Furthermore, Romboutsia timonensis was isolated from human colon (15), and ‘ Clostridium dakarense’ was isolated from the stool of a 4-month-old Senegalese child (16).…”

Screening, Mutagenesis of Nitrite‐Degrading Lactobacilli in Chinese Traditional Fermented Sauerkraut and its Application in the Production of Sauerkraut

“…The universal primers (with forward primer of 27f: 5′‐AGAGTT TGATCC TGG CTC AG‐3′ and reverse primer of 1522r: 5′‐AAG GAG GTG ATC CAG CCG CA‐3′) were used for amplification of 16S rRNA conserved sequence. The following standard amplification program was used: initial denaturation at 94°C for 5 min, followed by 35 cycles of denaturation at 94°C for 30 s, annealing at 56°C for 30 s and an extension at 72°C for 1 min (Moschonas and Bolton ). Sequencing of the polymerase chain reaction (PCR) products was completed by Sangon Biotech (Shanghai) Co., Ltd.…”