Characterization of a Potyvirus Infecting Sunflower in Argentina

Abstract

A virus causing chlorotic mottling symptoms on sunflower was found in various locations in Argentina. Symptoms were small chlorotic spots, yellow blotches on leaves, and plant stunting. Virus transmission efficiency by mechanical inoculation was 73 to 100%, and by Myzus persicae was 31 to 49%. The host range included members of the Amaranthaceae, Asteraceae, Chenopodiaceae, and Solanaceae families. Electron microscopy of leaf dips from infected plants revealed flexuous particles 17 nm wide and 770 nm long. Cytoplasmic laminar aggregates and pinwheel inclusions were observed in ultrathin sections. Purified virus preparations analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis resolved a capsid protein of 33 kDa. A monoclonal antibody to aphid-transmitted potyviruses reacted with the capsid protein of this virus. In dot blot immunoassays, a polyclonal antiserum (early bleeding) reacted with infected sunflowers and weakly with Bidens mottle potyvirus, but not with either maize dwarf mosaic potyvirus or potato virus Y. The evidence suggests that a potyvirus is infecting sunflower, and a partial characterization of the causal agent is reported.

Identificação e caracterização de um potyvírus isolado de Zinnia elegans

“…Algumas das hospedeiras (H. annuus, C. amaranticolor, C. quinoa, Z. elegans) bem como os sintomas induzidos pelo potyvírus de Zinnia nessas plantas, são similares àqueles apresentados pelo BiMV (Kuhn et al, 1980), BiMoV (Christie et al, 1968) e SuCMoV (Dujovny et al, 1998 A massa molecular da proteína capsidial (~33 kDa) está entre os valores obtidos para a maioria dos potyvírus (de 28 a 47 kDa) Hollings & Brunt, 1981) e foi similar aos valores encontrados para o SuCMoV (Dujovny et al, 1998) e para o BiMV (Nagata et al, 1995). Os resultados do "Western-blot" indicaram que o potyvírus de Zinnia possui relação sorológica com o BiMV.…”

“…Os resultados do "Western-blot" indicaram que o potyvírus de Zinnia possui relação sorológica com o BiMV. Levando-se em consideração que BiMV, BiMoV e SuCMoV são sorologicamente relacionados (Nagata et al, 1995;Dujovny et al, 1998), podese inferir que o potyvírus de Zinnia é também relacionado ao BiMoV e SuCMoV. Entretanto, a utilização da sorologia para determinação do grau de parentesco entre espécies de potyvírus não tem sido utilizada com sucesso, devido a ocorrência de reações cruzadas entre os vírus .…”

Alteraciones fisiológicas asociadas a la infección con Mal de Río Cuarto virus (MRCV) y a fitotoxicidad provocada por su insecto vector (Delphacodes kuscheli Fennah) en trigo

“…Al respecto, si bien no hay suficientes antecedentes en relación al efecto de los virus vegetales sobre la fisiología del hospedante, existen estudios que indican la ocurrencia de alteraciones relacionadas con la replicación viral y la expresión de síntomas. Así, por ejemplo, las infecciones por Sunflower chlorotic mottle virus (SuCMoV) causan moteado clorótico y notable reducción del crecimiento en plantas de girasol, síntomas que redundan en importantes disminuciones de rendimiento en esta oleaginosa y que son comunes a diversas enfermedades virales (Dujovny et al, 1998). Investigaciones recientes indican que dichos efectos estarían relacionados con una alteración en la síntesis de compuestos carbonados y con la ocurrencia de estrés oxidativo.…”

Amaranthus leaf mottle virus: 3′-end RNA sequence proves classification as distinct virus and reveals affinities within the genus Potyvirus

“…Viruses of the PVY cluster are closely phylogenetically related, and have a common ancestor (Spetz et al, 2003). Members from this aphidtransmitted virus group generally infect plants of the Solanaceae (order Solanales), but recently other natural hosts have been discovered, such as those belonging to the Alstroemeriaceae (Liliales) (Fuji et al, 2004), Amaryllidaceae (Asparagales) (Fuji et al, 2004), and Asteraceae (Asterales) (Dujovny et al, 1998). And now our research shows that the Amaranthaceae (Caryophyllales) is also a natural host.…”